E‑cadherin and NEDD9 expression in primary colorectal cancer, metastatic lymph nodes and liver metastases

Jurčić, Petra; Radulović, Petra; Balja, Melita Perić; Milošević, Milan; Krušlin, Božo

doi:10.3892/ol.2019.9917

Cited by 14 publications

(27 citation statements)

References 45 publications

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“…In a subgroup analysis of patients with and without lymph node metastases, there were no significant differences in Cx43 and NEDD9 expression in patients without lymph node metastases , but patients with lymph node metastases showed higher expression of epithelial NEDD9 (Table 1, Figure 2). (14,(16)(17)(18)(19). Similar as in our study, expression of NEDD9 was found significantly increased in the tissue of pancreatic ductal adenocarcinoma compared to adjacent pancreatic tissue (18), but the same was not established in colorectal cancer (19).…”

Section: Predicting 2-year Survivalsupporting

confidence: 82%

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Expression of NEDD9 and connexin-43 in neoplastic and stromal cells of gastric adenocarcinoma

Lerotić

Vuković

Hrabar

et al. 2021

Bosn J of Basic Med Sci

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Gastric cancer is related to high mortality rates and advanced disease stage at the time of diagnosis. Its carcinogenesis is extensively studied and is associated with genetic and epigenetic changes, changed the interaction between tumor and adjacent stromal cells, and changes in the microenvironment molecule status. NEDD9 affects different signaling proteins and pathways, apoptosis, adhesion, cell migration, and invasiveness. Connexin-43 also assists in intercellular communications and has several channel-independent functions. Aberrant expression of those two gap junction proteins plays an essential role in metastatic processes. Our scope was to detect the expression of connexin-43 and NEDD9 in epithelial and stromal gastric cancer compartments and its relation to tumor progression and lymph node metastases. Cancer tissue from 53 cases of node-negative and 55 cases of node-positive primary gastric carcinoma patients was analyzed for connexin-43 and NEDD9 expression by immunohistochemical assay, and the results were correlated with the remaining clinical and pathological findings and survival. In our cohort of patients with lymph node metastases, we detected higher expression of epithelial connexin-43 in the primary tumor and stromal connexin-43 expression correlated with both epithelial NEDD9 (rho=0.453) and stromal NEDD9 (rho=0.484). Higher epithelial connexin-43 and NEDD9 expression were associated with higher mortality (HR 1.54, 95% CI 1.01 -2.37, p=0.048). Epithelial connexin-43 expression, both epithelial and stromal NEDD9 expression, T and N status were all independently associated with shorter survival. In summary, our findings suggest that increased expression of both epithelial and stromal NEDD9 and epithelial connexin-43 could potentially be used as prognostic gastric cancer biomarkers.

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Section: Predicting 2-year Survivalsupporting

confidence: 82%

“…(14,(16)(17)(18)(19). Similar as in our study, expression of NEDD9 was found significantly increased in the tissue of pancreatic ductal adenocarcinoma compared to adjacent pancreatic tissue (18), but the same was not established in colorectal cancer (19).…”

Section: Predicting 2-year Survivalsupporting

confidence: 82%

Expression of NEDD9 and connexin-43 in neoplastic and stromal cells of gastric adenocarcinoma

Lerotić

Vuković

Hrabar

et al. 2021

Bosn J of Basic Med Sci

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“…The majority of published studies focused on identifying potential protein biomarkers using immunohistochemistry analysis of tissue samples or using enzyme-linked immunosorbent assay to measure their plasma concentrations. 25,30 Recent attention was given to circulating cell-free nucleic acids (cfNAs), such as DNA, mRNA or microRNA (miRNA), and cellular nucleic acids (cNAs). Currently, the most commonly used methods for cfRNAs and cNAs detection are qRT-PCR, microarray and deep sequencing.…”

Section: Discussionmentioning

confidence: 99%

“…22,23 However, there are limited data about EMT genes expression in patients with CRLM. 24,25 Moreover, relation and correlation between EMT genes expression in serum, metastatic CRC tissue or non-tumor liver tissue have been insufficiently investigated. The prognostic value of EMT markers in prediction of rapid disease progression after curativeintent treatment is still not elucidated.…”

Section: Introductionmentioning

confidence: 99%

Prognostic Significance of CDH1, FN1 and VIM for Early Recurrence in Patients with Colorectal Liver Metastasis After Liver Resection

Bogdanović¹,

Despotović²,

Galun³

et al. 2021

CMAR

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Purpose: There are limited data on expression of epithelial-mesenchymal transition (EMT) markers in patients with colorectal liver metastases (CRLM). The study aim was to evaluate the expression and prognostic significance of E-cadherin (CDH1), fibronectin (FN1) and vimentin (VIM) in patients with CRLM after curative-intent liver resection. Patients and Methods: Thirty patients with CRLM managed by curative-intent liver resection were included in this prospective pilot study. Blood samples, colorectal liver metastases and surrounding non-tumor liver tissue were collected. Expression of CDH1, FN1 and VIM was analyzed by quantitative real-time polymerase chain reaction. Expression in CRLM and non-tumor liver tissue was compared, while expression in serum was correlated with CRLM expression. One-year recurrence-free survival was compared between patients with low and high CDH1, FN1 and VIM expression. Results: The expression of CDH1 was similar in CRLM and non-tumor liver tissues, while FN1 and VIM expression was significantly lower in metastatic tissue (P=0.003 and pP<0.001, respectively). Serum expression of CDH1 and VIM was detected in 66.7% and 93.3% of patients, respectively, while FN1 was not detected in any of the patients. The correlation of CDH1 and VIM expression between CRLM and serum was not statistically significant. Decreased CDH1 expression in CRLM and decreased VIM expression in serum were associated with early recurrence after surgical treatment of CRLM. Conclusion: Lower expression of CDH1 in CRLM and lower serum expression of VIM were found to be associated with early recurrence after liver resection for CRLM.

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“…Finally, Jurčić et al, 48 reported no significant differences regarding the expression of E-cadherin in the primary tumor of CRC. Indeed, these results are similar to our own findings in that we did not observe significant differences in CDH1 expression in both AP and HP groups compared with normal participants.…”

Section: Discussionmentioning

confidence: 95%

APC and AXIN2 Are Promising Biomarker Candidates for the Early Detection of Adenomas and Hyperplastic Polyps

et al. 2020

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Aberrant activation of the WNT/CTNNB1 pathway is notorious in colorectal cancer (CRC). Here, we demonstrate that the expression of specific and crucial WNT signaling pathway genes is linked to disease progression in colonic adenomatous (AP) and hyperplastic (HP) polyps in an Iranian patient population. Thus, we highlight potential gene expression profiles as candidate novel biomarkers for the early detection of CRC. From a 12-month study (2016-2017), 44 biopsy samples were collected during colonoscopy from the patients with colorectal polyps and 10 healthy subjects for normalization. Clinical and demographic data were collected in all cases, and mRNA expression of APC, CTNNB1, CDH1, AXIN1, and AXIN2 genes was investigated using real-time polymerase chain reaction (PCR). CTNNB1 and CDH1 expression levels were unaltered in AP and HP subjects, whereas mRNA expression of APC was decreased in AP contrasted with HP subjects, with a significant association between APC downregulation and polyp size. Although AXIN1 showed no changes between AP and HP groups, a significant association between AXIN1 and dysplasia grade was found. Also, significant upregulation of AXIN2 in both AP and HP subjects was detected. In summary, we have shown increased expression of AXIN2 and decreased expression of APC correlating with grade of dysplasia and polyp size. Hence, AXIN2 and APC should be explored as biomarker candidates for early detection of AP and HP polyps in CRC.

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E‑cadherin and NEDD9 expression in primary colorectal cancer, metastatic lymph nodes and liver metastases

Cited by 14 publications

References 45 publications

Expression of NEDD9 and connexin-43 in neoplastic and stromal cells of gastric adenocarcinoma

Expression of NEDD9 and connexin-43 in neoplastic and stromal cells of gastric adenocarcinoma

Prognostic Significance of CDH1, FN1 and VIM for Early Recurrence in Patients with Colorectal Liver Metastasis After Liver Resection

APC and AXIN2 Are Promising Biomarker Candidates for the Early Detection of Adenomas and Hyperplastic Polyps

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