Prognostic Significance of CDH1, FN1 and VIM for Early Recurrence in Patients with Colorectal Liver Metastasis After Liver Resection

Abstract

Purpose: There are limited data on expression of epithelial-mesenchymal transition (EMT) markers in patients with colorectal liver metastases (CRLM). The study aim was to evaluate the expression and prognostic significance of E-cadherin (CDH1), fibronectin (FN1) and vimentin (VIM) in patients with CRLM after curative-intent liver resection. Patients and Methods: Thirty patients with CRLM managed by curative-intent liver resection were included in this prospective pilot study. Blood samples, colorectal liver metastases and surrounding non-tumor liver tissue were collected. Expression of CDH1, FN1 and VIM was analyzed by quantitative real-time polymerase chain reaction. Expression in CRLM and non-tumor liver tissue was compared, while expression in serum was correlated with CRLM expression. One-year recurrence-free survival was compared between patients with low and high CDH1, FN1 and VIM expression. Results: The expression of CDH1 was similar in CRLM and non-tumor liver tissues, while FN1 and VIM expression was significantly lower in metastatic tissue (P=0.003 and pP<0.001, respectively). Serum expression of CDH1 and VIM was detected in 66.7% and 93.3% of patients, respectively, while FN1 was not detected in any of the patients. The correlation of CDH1 and VIM expression between CRLM and serum was not statistically significant. Decreased CDH1 expression in CRLM and decreased VIM expression in serum were associated with early recurrence after surgical treatment of CRLM. Conclusion: Lower expression of CDH1 in CRLM and lower serum expression of VIM were found to be associated with early recurrence after liver resection for CRLM.

Label-free quantitative proteomics reveals aberrant expression levels of LRG, C9, FN, A1AT and AGP1 in the plasma of patients with colorectal cancer

“…In this study and a previous report by our group [ 10 ], plasma FN level is significantly decreased in CRC patients with the metastatic stage-dependent manner. Consistent with this finding, Bogdanovic et al showed that FN level was down-regulated in tissues and absent in the plasma of patients with CRC liver metastases [ 25 ]. Moreover, Zhou et al, reported that the serum FN level was decreased in patients with metastatic non-small cell lung cancer in comparison to those with non-metastatic lung cancer patients [ 26 ].…”

Multisite and multitimepoint proteomics reveal that patent foramen ovale closure improves migraine and epilepsy by reducing right‐to‐left shunt‐induced hypoxia

“…Reductions in the expression of adhesion molecules in neurons, such as PPM1H, NTSR2, CLDN10, and CDH13, are related to synaptic N‐methyl‐D‐aspartate (NMDA) receptor‐induced long‐term potentiation 69 and affect serotonin (5‐HT) formation, which is potentially relevant to migraine 70,71 . In addition, previous studies have shown that changes in the levels of CNTN1, CDH1, and other cell adhesion proteins in peripheral blood are associated with deficit schizophrenia, which may also be related to hypoxia 72,73 . We also found an increase in CAMs in blood after PFO closure; however, the clinical implications of this change remain to be explored in the future study.…”

Clinical significance of proliferation index and E-cadherin expression in colorectal adenocarcinoma

“…in the formation of a mutated, dysfunctional protein, which further results in decreased cell adhesion and uncontrolled growth of malignant cells [18]. Many studies have shown an association between reduced /absent E cadherin expression and progression of tumors [3,6].…”