e-Book on plant virus infection—a cell biology perspective

Laliberté, Jean‐François; Moffett, Peter; Sanfaçon, Hélène; Wang, Aiming; Nelson, Richard S.; Schoelz, James E.

doi:10.3389/fpls.2013.00203

Cited by 4 publications

(3 citation statements)

References 13 publications

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“…Both cell‐to‐cell and systemic movement are processes that require interaction between viral and host components (Niehl and Heinlein, ). To favour infection, plant viruses induce extensive host cell remodelling, such as the modification of PD structure (Laliberté et al ., ). For example, they have the capability to transiently increase the size exclusion limit (SEL) of PDs using virus‐encoded movement proteins (MPs), which allows viral passage into adjacent cells (Carrington et al ., ; Ghoshroy et al ., ; Lazarowitz and Beachy, ).…”

Section: Introductionmentioning

confidence: 97%

Transgenic expression of pectin methylesterase inhibitors limits tobamovirus spread in tobacco and Arabidopsis

Lionetti

Raiola

Cervone

et al. 2013

Molecular Plant Pathology

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Plant infection by a virus is a complex process influenced by virus-encoded factors and host components which support replication and movement. Critical factors for a successful tobamovirus infection are the viral movement protein (MP) and the host pectin methylesterase (PME), an important plant counterpart that cooperates with MP to sustain viral spread. The activity of PME is modulated by endogenous protein inhibitors (pectin methylesterase inhibitors, PMEIs). PMEIs are targeted to the extracellular matrix and typically inhibit plant PMEs by forming a specific and stable stoichiometric 1:1 complex. PMEIs counteract the action of plant PMEs and therefore may affect plant susceptibility to virus. To test this hypothesis, we overexpressed genes encoding two well-characterized PMEIs in tobacco and Arabidopsis plants. Here, we report that, in tobacco plants constitutively expressing a PMEI from Actinidia chinensis (AcPMEI), systemic movement of Tobacco mosaic virus (TMV) is limited and viral symptoms are reduced. A delayed movement of Turnip vein clearing virus (TVCV) and a reduced susceptibility to the virus were also observed in Arabidopsis plants overexpressing AtPMEI-2. Our results provide evidence that PMEIs are able to limit tobamovirus movement and to reduce plant susceptibility to the virus.

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Section: Introductionmentioning

confidence: 97%

Transgenic expression of pectin methylesterase inhibitors limits tobamovirus spread in tobacco and Arabidopsis

Lionetti

Raiola

Cervone

et al. 2013

Molecular Plant Pathology

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“…Previous research has demonstrated that viral infection can alter the molecular function, biological processes and cellular components of host plants [ 71 ]. The response of cucumber to viral stress is complex and involves many genes and molecular mechanisms, operating at both the transcriptional and post-transcriptional level.…”

Section: Discussionmentioning

confidence: 99%

High-Throughput Sequencing Identifies Novel and Conserved Cucumber (Cucumis sativus L.) microRNAs in Response to Cucumber Green Mottle Mosaic Virus Infection

et al. 2015

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Seedlings of Cucumis sativus L. (cv. 'Zhongnong 16') were artificially inoculated with Cucumber green mottle mosaic virus (CGMMV) at the three-true-leaf stage. Leaf and flower samples were collected at different time points post-inoculation (10, 30 and 50 d), and processed by high throughput sequencing analysis to identify candidate miRNA sequences. Bioinformatic analysis using screening criteria, and secondary structure prediction, indicated that 8 novel and 23 known miRNAs (including 15 miRNAs described for the first time in vivo) were produced by cucumber plants in response to CGMMV infection. Moreover, gene expression profiles (p-value <0.01) validated the expression of 3 of the novel miRNAs and 3 of the putative candidate miRNAs and identified a further 82 conserved miRNAs in CGMMV-infected cucumbers. Gene ontology (GO) analysis revealed that the predicted target genes of these 88 miRNAs, which were screened using the psRNATarget and miRanda algorithms, were involved in three functional categories: 2265 in molecular function, 1362 as cellular components and 276 in biological process. The subsequent Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that the predicted target genes were frequently involved in metabolic processes (166 pathways) and genetic information processes (40 pathways) and to a lesser degree the biosynthesis of secondary metabolites (12 pathways). These results could provide useful clues to help elucidate host-pathogen interactions in CGMMV and cucumber, as well as for the screening of resistance genes.

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“…His principal interests are the study of enzyme and immunoglobulin structure, and the structures of viruses using the techniques of X-ray crystallography and atomic force microscopy. 45 Step bunches, 16 Step edge movement, 20 Step edges, 15 …”

Section: Refinement Of Virus Crystal Structuresmentioning

confidence: 99%

A guide to the crystallographic analysis of icosahedral viruses

McPherson

Larson

2015

Crystallography Reviews

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Determining the structure of an icosahedral virus crystal by X-ray diffraction follows very much the same course as conventional protein crystallography. The major differences arise from the relatively large sizes of the particles, which significantly affect the data collection process, data processing and management, and later, the refinement of a model. Most of the other differences are due to the high 5 3 2 point group symmetry of icosahedral viruses. This alters dramatically the means by which initial phases are obtained by molecular substitution, extended to higher resolution by electron density averaging and density modification, and the refinement of the structure in the light of high non-crystallographic symmetry. In this review, we attempt to lead the investigator through the various steps involved in solving the structure of a virus crystal. These steps include the purification of viruses, their crystallization, the recording of X-ray diffraction data, and its reduction to structure amplitudes. It further addresses the problems attending phase determination and ultimately the refinement of a model. Finally, we describe the unique properties of virus crystals and the factors that influence their physical and diffraction properties.

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e-Book on plant virus infection—a cell biology perspective

Cited by 4 publications

References 13 publications

Transgenic expression of pectin methylesterase inhibitors limits tobamovirus spread in tobacco and Arabidopsis

Transgenic expression of pectin methylesterase inhibitors limits tobamovirus spread in tobacco and Arabidopsis

High-Throughput Sequencing Identifies Novel and Conserved Cucumber (Cucumis sativus L.) microRNAs in Response to Cucumber Green Mottle Mosaic Virus Infection

A guide to the crystallographic analysis of icosahedral viruses

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