Dysbiosis of urinary microbiota is positively correlated with Type 2 diabetes mellitus

Liu, Fengping; Ling, Zongxin; Xiao, Yonghong; Lv, Longxian; Yang, Qing; Wang, Baohong; Lü, Haifeng; Zheng, Li; Jiang, Ping; Wang, Wei; Li, Lanjuan

doi:10.18632/oncotarget.14028

Cited by 43 publications

(61 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Three bands were identified as Proteobacteria and the other one band was Firmicutes. The occurrence of these bacterial phyla has also been reported previously in various studies by using either culture-dependent methods (Liu et al 2017). In our study, we observed bacterial isolates belong to Gammaproteobacteria, these included Shigella flexneri, Klebsiella sp., and Escherichia sp.…”

Section: The Diversity Of Bacteria Based On Metagenomic 16s Rrnatargesupporting

confidence: 86%

Diversity of urinary tract infection bacteria in children in Indonesia based on metagenomic approach

2018

View full text Add to dashboard Cite

Christine G, Budiarti S, Astuti RI. 2018. Diversity of urinary tract infection bacteria in children in Indonesia based onmetagenomic approach. Biodiversitas 19: 1375-1381. Urinary tract infection (UTI) is a common bacterial infection in childhood in bothmales and females. The infection is usually caused by bacterial invasion of the urinary tract including the lower and the upper urinarytract. In Indonesia, to the best our knowledge, the diversity of urinary tract infection bacteria has not been reported yet. Therefore, theaims of this study were to identify the diversity of both culturable and unculturable bacteria in children diagnosed with UTI. In thisstudy, urine samples were obtained from different age groups ranging 6-17 years. Analysis of 16S rRNA gene sequence showed eightculturable isolates (SBU1, SBU2, SBU3, SBU4, SBU5, SBU6, SBU7, SBU8) are closely related to Escherichia coli, Klebsiella pneumoniae,Enterobacter aerogenes, Citrobacter sp. and Acinetobacter sp. with maximum identity up to 98-99%. Diversity of unculturable bacteriacommunity based on 16S rRNA gene was represented by 9 DGGE (Denaturing Gradient Gel Electrophoresis) bands. The ninerespective bands showed the similarity ranging from 84 up to 96% with Klebsiella sp, Escherichia sp., Lactococcus lactic, Shigellaflexneri and uncultured bacterium. Based on phylogenetic analysis, all culturable isolates belong to phylum Proteobacteria, which isdominated by family of Enterobacteriaceae. Interestingly, by using metagenomic approach, it is observed that bacteria belong to phylumFirmicutes were found in the UTI-diagnosed patients, in addition to those bacterial isolates from phylum Proteobacteria. To ourknowledge, this is the first study to report the occurrence of Firmicutes and Proteobacteria in UTI-diagnosed patients in Indonesia.

show abstract

Section: The Diversity Of Bacteria Based On Metagenomic 16s Rrnatargesupporting

confidence: 86%

Diversity of urinary tract infection bacteria in children in Indonesia based on metagenomic approach

2018

View full text Add to dashboard Cite

show abstract

“…The most abundant phylum in both bladder cancer and healthy group was Firmicutes, followed by Actinobacteria, Bacteroidetes and Proteobacteria (Fig. 2), which is in good Also, inter-individual variability, observed between microbiomes of the participants in our study, has been repeatedly demonstrated in publications on urinary microbiome 10,11,19,20 , which makes it challenging to define what constitutes a 'core' bladder microbiome. Our comparison of bladder cancer versus healthy urine samples revealed bacterial taxa that 1 1 6 were overrepresented in one of the sample subgroups ( Fig.…”

Section: Introductionsupporting

confidence: 59%

The urinary microbiome associated with bladder cancer

Bučević-Popović

Šitum

Chow

et al. 2017

Preprint

View full text Add to dashboard Cite

Recent findings suggest that microorganisms inhabiting the human body can influence the 1 development of cancer, but the role of microorganisms in bladder cancer pathogenesis has not 2 been explored yet. The aim of this study was to characterize and compare the urinary 3 microbiome of bladder cancer patients with those of healthy controls. Bacterial communities 4 present in urine specimens collected from male patients diagnosed with primary or recurrent, 5 non-muscle invasive bladder cancers, and from healthy, age-matched individuals were 6 analysed using 16S Illumina MiSeq sequencing. Our result show that the most abundant 7 phylum in both groups was Firmicutes, followed by Actinobacteria, Bacteroidetes and pathogen. Three OTUs more abundant in healthy urines were from genera Veillonella,

show abstract

“…In the past, urine was thought to be sterile in healthy individuals. Recent studies show that there are a great number of microorganisms in the urinary tract [1] and changes in patients with type 2 diabetes mellitus [2], overactive bladder syndrome [3,4], urinary incontinence, interstitial cystitis [5], neuropathic bladder [6,7], sexually transmitted infections [8], and chronic prostatitis/chronic pelvic pain syndrome [9,10].…”

Section: Introductionmentioning

confidence: 99%

The Microbiome in Benign Renal Tissue and in Renal Cell Carcinoma

et al. 2019

View full text Add to dashboard Cite

Introduction: The renal bacterial colonization has not been explored so far. Objective: The aim of this study was to describe the renal microbiome and to determine differences of the renal microbiome in healthy and tumor-bearing parenchyma. Methods: Ten biopsies from patients undergoing laparoscopic nephrectomy for renal carcinoma with no history of urinary tract infections within the last 6 months were included in this study. The identification of all microorganisms was done using 16S DNA sequencing. The beta diversity analysis was performed by Bray-Curtis dissimilarity. Results: In all kidney samples, a plethora of microorganisms was found, with significant differences between benign and malignant renal tissue (p < 0.0001). Conclusions: There is evidence that healthy kidney tissue as well as renal cell cancer tissue have a specific microbiome, thus opening new perspectives in renal physiology and tumor pathogenesis.hypothesis that bacterial colonization of the kidney differs even within the very same organ if parts are affected by disease.

show abstract

Dysbiosis of urinary microbiota is positively correlated with Type 2 diabetes mellitus

Cited by 43 publications

References 60 publications

Diversity of urinary tract infection bacteria in children in Indonesia based on metagenomic approach

Diversity of urinary tract infection bacteria in children in Indonesia based on metagenomic approach

The urinary microbiome associated with bladder cancer

The Microbiome in Benign Renal Tissue and in Renal Cell Carcinoma

Contact Info

Product

Resources

About