Diversity of urinary tract infection bacteria in children in Indonesia based on metagenomic approach

Christine, Grace; Budiarti, Sri; Astuti, Rika Indri

doi:10.13057/biodiv/d190425

Cited by 4 publications

(4 citation statements)

References 19 publications

(19 reference statements)

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“…Citrobacter braakii used in this study was obtained from the urine of UTI-diagnosed children, which has been isolated from our earlier study (Christine et al 2018). Bacterial isolate was routinely culture in Luria Bertani agar (LA) medium.…”

Section: Bacterial Isolate Source and Sample Collectionmentioning

confidence: 99%

“…Citrobacter strains are the third most common bacteria causing UTI in hospitalized patients besides Escherichia coli and Klebsiella strains up to 9.4% of all bacterial isolates obtained (Metri et al 2013). A previous study through a culture-based approach also revealed that C. braakii has been found in urine from UTI-diagnosed children (Christine et al 2018). Therefore, it is an emergence to find an effective treatment strategy to control the infection cases caused by this bacterium.…”

Section: Introductionmentioning

confidence: 99%

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Short Communication: The potency of lytic bacteriophage isolated from various environments to control the growth of Citrobacter braakii causing urinary tract infection

et al. 2021

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Abstract. Sheilahrusi, Priyanto JA, Rismiyati H, Rusmana I, Budiarti S. 2021. Short Communication: The potency of lytic bacteriophage isolated from various environments to control the growth of Citrobacter braakii causing urinary tract infection. Biodiversitas 22: 5550-5554. Citrobacter braakii is one of the pathogenic bacteria causing urinary tract infection (UTI) in humans. Bacteriophages that are specifically infecting C. braakii could be the alternatives to combat antibiotics resistance cases of this bacterium. This study aimed to isolate lytic phages from various environmental samples (tofu factory wastewater, sewage water, rice field water, fishpond water, cattle farm wastewater, and goat farm wastewater), and to analyze their effectivity to reduce UTI-causing C. braakii population in vitro. Results exhibited that phages targeting this bacterium were found in goat and cattle farm wastewater and not present in other samples. Two phages, namely FC1 and FC2, had different plaque morphology characteristics. The number of phages in cattle farm wastewater was 2.8 × 105 PFU/mL and 1.32 × 104 PFU/mL in goat farm wastewater. It was observed that the phages found in these environments also indicate the presence of their host. After 8 h incubation, FC1 and FC2 phage reduced UTI-causing C. braaakii population in vitro from 0.23×108 CFU/mL to 0.03×108 CFU/mL and 0.02×108 CFU/mL, respectively. The phages isolated from these two samples can be further developed for the treatment of UTI caused by C. braakii and goat and cattle farm wastewater treatment to prevent contaminating other areas.

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Section: Bacterial Isolate Source and Sample Collectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Short Communication: The potency of lytic bacteriophage isolated from various environments to control the growth of Citrobacter braakii causing urinary tract infection

et al. 2021

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“…It means that this bacteria group is still potential to be used as a source of novel antibacterial compound for some infectious diseases. Christine et al (2018) reported that Escherichia coli, Acinetobacter calcoaceticus, Citrobacter braakii, and Klebsiella pneumoniae were found in UTI urine patients. In accordance with the requirement of new antibacterial compounds and the existence of actinomycetes in rhizosphere, this research conducted some methods to find novel antibacterial compounds from actinomycetes for bacteria from UTI patients as a target.…”

Section: Introductionmentioning

confidence: 99%

Actinomycetes of rhizosphere soil producing antibacterial compounds against Urinary Tract Infection bacteria

2019

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Abstract. Apsari PP, Budiarti S, Wahyudi AT. 2019. Actinomycetes of rhizosphere soil producing antibacterial compounds against Urinary Tract Infection bacteria. Biodiversitas 20: 1259-1265. Based on the ability of actinomycetes as an antibacterial compounds producer and the need of finding novel antibacterial compounds, this study aims to look for antibacterial compounds from rhizosphere actinomycetes against bacteria in urine of UTI patients (Escherichia coli, Citrobacter braakii, Acinetobacter calcoaceticus, and Klebsiella pneumoniae). The screening of 21 actinomycetes was conducted based on the paper disc method. Potential actinomycetes that inhibited test bacteria were, then cultured in liquid medium and the supernatant was tested to six test bacteria. Then, the supernatant was extracted using ethyl acetate and crude extract from extraction process was tested to test bacteria. Afterward, the crude extract was scanned for bioactive compounds by GC-MS. Potential actinomycetes were identified by 16S rRNA gene to reveal the species. The screening results showed that ARJ 16, 24, and 36 had a wider inhibition zone than others. All of them showed that the supernatant and the crude extract could inhibit UTI's bacteria. The highest abundance of bioactive compound of crude extract was found in Propane, 1,2-dichloro, n-Hexadecanoic acid, and Carbonochloridic acid, 2-chloroethyl ester, respectively. Identification of potential actinomycetes based on 16S rRNA gene showed that ARJ 16 and ARJ 24 were highly similar to Streptomyces sp. in 99% and ARJ 36 was similar to Streptomyces tendae in 98%.

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“…Some urine samples from different childhood's patient in Indonesia also showed the presence of culturable UPEC and unculturable Escherichia sp. based on 16S rRNA gene (Christine et al 2018). Mechanism of UPEC in infecting urinary tract is commonly initiated by colonizing E. coli from anus to periurethral slot, then it moves to bladder and kidney by ascending.…”

Section: Introductionmentioning

confidence: 99%

Diversity of uropathogenic Escherichia coli lytic phage from Cisadane River, West Java, Indonesia based on morphology and protein molecular weight characteristics

2018

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Sufa HI, Budiarti S, Rusmana I. 2018. Diversity of uropathogenic Escherichia coli lytic phage from Cisadane River, West Java, Indonesia based on morphology and protein molecular weight characteristics. Biodiversitas 19: 2359-2364. The aimed of this study was to identify, isolate and characterized the lytic phage diversity which had a potential in reducing uropathogenic Escherichia coli (UPEC), antibiotic resistant-pathogenic bacteria infecting urinary tract from Cisadane river based on the morphology and protein molecule weight. To the best of our knowledge, this study has not yet been reported in Indonesia. Two novel lytic phages of UPEC, FU1, and FU3 were isolated from Cisadane River, Indonesia. Both of lytic phages were more closely related to Podoviridae, which had icosahedral capsid in 47.2 nm and 40 nm diameter length, respectively and short-noncontractile tail (< 40 nm). The FU1 and FU3 had different protein molecular weight ranges between 10-76 kDa and 8-120 kDa, respectively. In addition, the bacteriolytic activity of FU3 (1.6 ´ 107 PFU/mL) was able to reduce UPEC (1.7 ´ 107 CFU/mL) to become lower than the limitation value of infection (< 105 CFU/mL) in only 25 h of incubation. A better effect of FU3 phage in decreasing UPEC population in vitro is expected to be one of the further alternative biocontrol agents to reduce the abundance of UPEC population which grows in urinary tract or contaminates river water.

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Diversity of urinary tract infection bacteria in children in Indonesia based on metagenomic approach

Cited by 4 publications

References 19 publications

Short Communication: The potency of lytic bacteriophage isolated from various environments to control the growth of Citrobacter braakii causing urinary tract infection

Short Communication: The potency of lytic bacteriophage isolated from various environments to control the growth of Citrobacter braakii causing urinary tract infection

Actinomycetes of rhizosphere soil producing antibacterial compounds against Urinary Tract Infection bacteria

Diversity of uropathogenic Escherichia coli lytic phage from Cisadane River, West Java, Indonesia based on morphology and protein molecular weight characteristics

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