Actinomycetes of rhizosphere soil producing antibacterial compounds against Urinary Tract Infection bacteria

Abstract

Abstract. Apsari PP, Budiarti S, Wahyudi AT. 2019. Actinomycetes of rhizosphere soil producing antibacterial compounds against Urinary Tract Infection bacteria. Biodiversitas 20: 1259-1265. Based on the ability of actinomycetes as an antibacterial compounds producer and the need of finding novel antibacterial compounds, this study aims to look for antibacterial compounds from rhizosphere actinomycetes against bacteria in urine of UTI patients (Escherichia coli, Citrobacter braakii, Acinetobacter calcoaceticus, and Klebsiella pneumoniae). The screening of 21 actinomycetes was conducted based on the paper disc method. Potential actinomycetes that inhibited test bacteria were, then cultured in liquid medium and the supernatant was tested to six test bacteria. Then, the supernatant was extracted using ethyl acetate and crude extract from extraction process was tested to test bacteria. Afterward, the crude extract was scanned for bioactive compounds by GC-MS. Potential actinomycetes were identified by 16S rRNA gene to reveal the species. The screening results showed that ARJ 16, 24, and 36 had a wider inhibition zone than others. All of them showed that the supernatant and the crude extract could inhibit UTI's bacteria. The highest abundance of bioactive compound of crude extract was found in Propane, 1,2-dichloro, n-Hexadecanoic acid, and Carbonochloridic acid, 2-chloroethyl ester, respectively. Identification of potential actinomycetes based on 16S rRNA gene showed that ARJ 16 and ARJ 24 were highly similar to Streptomyces sp. in 99% and ARJ 36 was similar to Streptomyces tendae in 98%.

Identification of antagonistic Streptomyces strains isolated from Algerian Saharan soils and their plant growth promoting properties

“…The majority of rhizospheric actinobacteria are Streptomyces (66%) (Apsari et al 2019). They are characterized by their complex life cycle and high GC content (more than 70%) (Lee et al 2020).…”

Cliona varians-Derived Actinomycetes as Bioresources of Photoprotection-Related Bioactive End-Products

“…and the bacterium B. subtilis, discarding the streptomycete as the source [51]. However, 14 has been previously identified in Streptomyces by LC-MS-based annotation [52]. In addition, we also putatively identified other compounds that were not previously reported in any streptomycete.…”

Antimicrobial activity of ethyl acetate extracts of Streptomyces sp. CRB46 and the prediction of their bioactive compounds chemical structure

“…Therefore, semi-polar ethyl acetate was chosen as a solvent in extraction, both for EAS and EAP extraction. Previous studies also used ethyl acetate as the solvent in the extraction of Actinomycetes from rhizosphere soil from mangrove (Retnowati et al 2018), Streptomyces omiyaensis SCH2 from mangrove sediment (Tangjitjaroenkun 2018) and Actinomycetes from maize rhizosphere soil in Nusa Tenggara Timur (Apsari et al 2019). The percentage yield of EAS extract was 20%, much higher than the percentage yield (2.89%) of EAP extract.…”

“…The bioautography results show that the active compound from one isolate, namely FUAm2-h1, has an Rf value of 0.94 (Retnowati et al 2018). Another study has shown that the supernatant crude extract of rhizospheric actinomycetes also inhibits the growth of urinary tract infections bacteria (Apsari et al 2019).…”