Dynamism of Antimicrobial Activity of Actinomycetes—A Case Study from Undisturbed Microbial Niche

Patel, J. K.; Parmar, Mahesh; Patel, Purshottam; Rohit, Pravin; Taviyad, Ramesh; Ansari, Parveen; Bhattacharya, Bipasha; Vyas, Dhaval K.; Kumar, Vipin; Sahay, Nirmal S.; Singh, Pawan

doi:10.4236/aim.2014.46039

Cited by 10 publications

(9 citation statements)

References 20 publications

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“…Patil et al [38] reported that 77 isolates out of 104 were found to be inhibitory to at least one of the pathogens. Also, Patel et al [39] reported that 79% of the actinomycetes isolates were active against at least one of the 18 tested pathogens. In a recent study, Rana and Salam [9] characterized 23 actinomycetes isolates, 7 of which showed significant inhibition of different Gram positive and Gram negative bacteria and also some fungi, with zones of inhibition up to 20 mm.…”

Section: Screening For Antibacterial Activitymentioning

confidence: 99%

Antibacterial and anticancer activity of marineStreptomyces parvus: optimization and application

Abd-Elnaby

Abo-Elala

Abdel-Raouf

et al. 2015

Biotechnology & Biotechnological Equipment

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A total of 17 actinomycetes were isolated and screened against five bacterial pathogens. Forty-one per cent of the isolates were active against the tested pathogens. The most potent isolate was identified as Streptomyces parvus by using a 16S rRNA sequence analysis. S. parvus produced active compound(s) against a number of Gram negative and Gram positive bacteria. The obtained inhibition zones were 14, 19, 20 and 20 mm against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Aeromonas hydrophila, respectively. Moreover, the anticancer activity of S. parvus was tested against four different cell lines: human liver cancer cell line, mouse lymphoma cell line, breast cancer cell line and human colon cancer cell line. The inhibition activities were 53%, 56%, 57% and 42%, respectively. To achieve a maximum production of the bioactive compound, PlackettÀBurman design was applied. The productivity increased up to 1.3-fold, when S. parvus was grown in optimized medium composed of: 10 g, 2 mL (10 3 colony-forming units mL ¡1 ) inoculum size with pH 8 for 7 d of incubation. The main constitutes of S. parvus crude extract were determined by gasÀliquid chromatography mass spectrometry. They were found to be ethane, 1,1-diethoxy; di-n-octyl phthalate; ethanol, 2,2-diethoxy; 9,12-octadecadienoic acid; methyl ester (E,E) and benzoic acid.

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Section: Screening For Antibacterial Activitymentioning

confidence: 99%

Antibacterial and anticancer activity of marineStreptomyces parvus: optimization and application

Abd-Elnaby

Abo-Elala

Abdel-Raouf

et al. 2015

Biotechnology & Biotechnological Equipment

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“…In the current study, the isolated actinomycetes showed high antagonistic activity against several pathogenic fungi and bacteria. Patel et al (2014) reported that 79% of the actinomycetes isolates were active against at least one of the 18 tested pathogens. Other reports indicated that actinomycetes isolates showed inhibitory activity against mycelial growth of plant pathogenic fungi like Alternaria mali, Colletotrichum gloeosporides, F. oxysporum (Lim et al, 2000); against yeasts and gram positive and gram negative antibiotic resistant pathogens (Thirumalairaj et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Molecular identification of actinomycetes with antimicrobial, antioxidant and anticancer properties

Abdel‐Aziz¹,

Hathout²,

El-Neleety³

et al. 2019

Com. Sci.

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The objectives of this study were to isolate and identify the actinomycetes strains from the soil and marine sediments and to evaluate the antimicrobial, antioxidant and cytotoxic activity of their bioactive secondary metabolites. Eight actinomycetes strains were isolated from soil and marine sediment samples collected from different areas in Egypt. Only three actinomycetes exhibited a wide spectrum of antimicrobial activities. They were active in vitro against microbial pathogen viz: Staphylococcus aureus, Escherichia coli, Salmonella typhi, Aspergillus parasiticus, Fusarium solani and Fusarium oxysporum. These promising isolates were selected and identified using molecular identification technique and identified as Streptomyces spp. The crude extracts from the three Streptomyces exhibited potent antimicrobial activities against a set of microbial pathogens as well as antioxidant and anticancer activity in human hepatocellular carcinoma cell line (HepG2). The crude extract of Streptomyces isolate D showed antitumor activity with lC50 0.85 µg/ mL. Forty compounds were identified from the two most promising ethyl acetate extracts of culture broth of Streptomyces sp. (D-EGY) by Gas Chromatography-Mass Spectrometry analysis. It could be concluded that the streptomycetes isolated from the Egyptian environment are promising candidates as antimicrobial, antioxidant and anticancer.

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“…All the isolates were gram positive and showed different morphological characteristics on half strength Nutrient agar [12]. Many researchers have successfully isolated actinomycetes from various sources like [15], termite gut [16], plant [17], mud [18] and marine [19].…”

Section: Isolation and Culture Characteristicsmentioning

confidence: 99%

Bioactive Metabolites from Indigenous Actinomycetes Isolated from Marine Water

Subhan

Wahab

Mujahid

et al. 2015

J. Pharm. Nutr. Sci.

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Microbial natural products have continued to play an important role in the discovery of novel chemicals for the development of important therapeutic agents. Actinomycetes form a potent reservoir of biologically active secondary metabolites and enzymes. The need for finding novel bioactive compounds for the development of new therapeutic agents is required due to the emergence of antibiotic resistance among pathogenic bacteria. Actinomycetes are considered as one of the best producers of variety of antagonistic compounds that could serve as potential chemotherapeutic agents. The present study was undertaken to find new antagonistic compounds from actinomycetes. Actinomycetes were successfully isolated from marine water samples collected at various locations of Karachi. Initially 39 isolates were collected out of which 23 were found to produce active metabolites against one or more test bacterial cultures. Actinomycetes strains IS26, IS33, and IS39 showed significant potential of having bioactive metabolites. Further, the spectrum of those strains was tested against gram positive and gram negative bacteria and results showed variable potential of actinomycetes to inhibit bacterial growth.

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Dynamism of Antimicrobial Activity of Actinomycetes—A Case Study from Undisturbed Microbial Niche

Cited by 10 publications

References 20 publications

Antibacterial and anticancer activity of marineStreptomyces parvus: optimization and application

Antibacterial and anticancer activity of marineStreptomyces parvus: optimization and application

Molecular identification of actinomycetes with antimicrobial, antioxidant and anticancer properties

Bioactive Metabolites from Indigenous Actinomycetes Isolated from Marine Water

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