Dynamism in physiology and gene transcription during reproductive diapause in a heteropteran bug, Pyrrhocoris apterus

Košťál, Vladimı́r; Tollarová, Michaela; Doležel, David

doi:10.1016/j.jinsphys.2007.08.004

Cited by 54 publications

(18 citation statements)

References 55 publications

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“…Doležel et al (2007) revealed that this different level in per mRNA between diapause and reproductive females was highest 3 to 5 days after adult ecdysis and disappeared 14 days after adult ecdysis. Such distinct suppression at the early phase of adult stage was also observed in cry expression (Koštál et al, 2008). These results show that the alteration of mRNA levels of circadian clock genes is indispensable for the expression of diapause in P. apterus.…”

Section: Discussionsupporting

confidence: 66%

Molecular characterization of the circadian clock genes in the bean bug, Riptortus pedestris, and their expression patterns under long- and short-day conditions

Ikeno

Numata

Goto

2008

Gene

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Section: Discussionsupporting

confidence: 66%

Molecular characterization of the circadian clock genes in the bean bug, Riptortus pedestris, and their expression patterns under long- and short-day conditions

Ikeno

Numata

Goto

2008

Gene

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“…Diapause-related changes in SDH expression is also observed in other insects. In Bombyx mori, SDH is expressed late during embryonic diapause [40], [41] and declines during the termination of diapause in the adult heteropteran bug, Pyrrhocoris apterus [42]. In Wyeomyia smithii , SDH-1 and SDH-2 are highly expressed during larval diapause, and are down-regulated after termination of diapause [43].…”

Section: Discussionmentioning

confidence: 99%

Global Transcriptome Analysis of Orange Wheat Blossom Midge, Sitodiplosis mosellana (Gehin) (Diptera: Cecidomyiidae) to Identify Candidate Transcripts Regulating Diapause

Jin

Duan

et al. 2013

PLoS ONE

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BackgroundMany insects enter a developmental arrest (diapause) that allows them to survive harsh seasonal conditions. Despite the well-established ecological significance of diapause, the molecular basis of this crucial adaptation remains largely unresolved. Sitodiplosis mosellana (Gehin), the orange wheat blossom midge (OWBM), causes serious damage to wheat throughout the northern hemisphere, and sporadic outbreaks occur in the world. Traits related to diapause appear to be important factors contributing to their rapid spread and outbreak. To better understand the diapause mechanisms of OWBM, we sequenced the transcriptome and determined the gene expression profile of this species.Methodology/Principal FindingsIn this study, we performed de novo transcriptome analysis using short-read sequencing technology (Illumina) and gene expression analysis with a tag-based digital gene expression (DGE) system. The sequencing results generated 89,117 contigs, and 45,713 unigenes. These unigenes were annotated by Blastx alignment against the NCBI non-redundant (nr), Clusters of orthologous groups (COG), gene orthology (GO), and the Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. 20,802 unigenes (45.5% of the total) matched with protein in the NCBI nr database. Two digital gene expression (DGE) libraries were constructed to determine differences in gene expression profiles during diapause and non-diapause developmental stages. Genes related to diapause were analyzed in detail and in addition, nine diapause-related genes were analyzed by real time PCR.Conclusions/SignificanceThe OWBM transcriptome greatly improves our genetic understanding and provides a platform for functional genomics research of this species. The DGE profiling data provides comprehensive information at the transcriptional level that facilitates our understanding of the molecular mechanisms of various physiological aspects including development and diapause stages in OWBM. From this study it is evident that various genes coding metabolic enzymes are crucial for diapause and metamorphosis.

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“…AKRs are monomeric proteins with NADPH-dependent catalytic activity. One member of this family is known to be upregulated during diapause initiation in the heteropteran bug, Pyrrhocoris apterus (Kostál et al, 2008). Biochemical studies on ecdysteroid metabolism in insects and crustaceans suggest that aldo-ketoreductase enzymes may be involved in this pathway (Maibeche-Coisne et al, 2001;Sieglaff et al, 2005).…”

Section: Endocrine and Signal Transductionmentioning

confidence: 99%

Embryonic diapause highlighted by differential expression of mRNAs for ecdysteroidogenesis, transcription and lipid sparing in the cricketAllonemobius socius

Reynolds

Hand

2009

Journal of Experimental Biology

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Dynamism in physiology and gene transcription during reproductive diapause in a heteropteran bug, Pyrrhocoris apterus

Cited by 54 publications

References 55 publications

Molecular characterization of the circadian clock genes in the bean bug, Riptortus pedestris, and their expression patterns under long- and short-day conditions

Molecular characterization of the circadian clock genes in the bean bug, Riptortus pedestris, and their expression patterns under long- and short-day conditions

Global Transcriptome Analysis of Orange Wheat Blossom Midge, Sitodiplosis mosellana (Gehin) (Diptera: Cecidomyiidae) to Identify Candidate Transcripts Regulating Diapause

Embryonic diapause highlighted by differential expression of mRNAs for ecdysteroidogenesis, transcription and lipid sparing in the cricketAllonemobius socius

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