Dynamics of the human skin mediator lipidome in response to dietary ω‐3 fatty acid supplementation

Kendall, Alexandra C.; Pilkington, Suzanne M.; Murphy, Sharon Ann; Carratore, Francesco Del; Sunarwidhi, Anggit Listyacahyani; Kiezel-Tsugunova, Magdalena; Urquhart, Paula; Watson, Rachel; Breitling, Rainer; Rhodes, Lesley E.; Nicolaou, Anna

doi:10.1096/fj.201901501r

Cited by 29 publications

(50 citation statements)

References 61 publications

(92 reference statements)

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“…Fourth, non-fasting plasma samples were analyzed. Certain lipid mediators including NAEs can be altered with diet ( 58 , 59 ). These issues may have diminished our power to detect genetic associations, although some may not have major impact, for example, previous studies of blood CER using lipidomics analyses did not find differences between serum and plasma samples, and fasting and non-fasting samples ( 50 , 60 , 61 ).…”

Section: Discussionmentioning

confidence: 99%

“…Plasma samples were extracted and analyzed by mass spectrometry as previously described ( 59 , 66 , 68 ). Briefly, lipids were extracted from plasma (1 mL) using chloroform–methanol in the presence of internal standards: CER[N(25)S(18)] (50 pmol/sample; Ceramide/Sphingoid Internal Standard Mixture I, Avanti Polar Lipids, USA) for CER and AEA- d 8 (20 ng/sample; Cayman Chemical Co., USA) for NAE.…”

Section: Methodsmentioning

confidence: 99%

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Heritability and family-based GWAS analyses of the N-acyl ethanolamine and ceramide plasma lipidome

McGurk

Williams

Guo

et al. 2021

Human Molecular Genetics

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Signalling lipids of the N-acyl ethanolamine (NAE) and ceramide (CER) classes have emerged as potential biomarkers of cardiovascular disease (CVD). We sought to establish the heritability of plasma NAEs (including the endocannabinoid anandamide) and CERs, to identify common DNA variants influencing the circulating concentrations of the heritable lipids, and assess causality of these lipids in CVD using 2-sample Mendelian randomization (2SMR). Nine NAEs and 16 CERs were analyzed in plasma samples from 999 members of 196 British Caucasian families, using targeted ultra-performance liquid chromatography with tandem mass spectrometry. All lipids were significantly heritable (h2 = 36–62%). A missense variant (rs324420) in the gene encoding the enzyme fatty acid amide hydrolase (FAAH), which degrades NAEs, associated at genome-wide association study (GWAS) significance (P < 5 × 10−8) with four NAEs (DHEA, PEA, LEA and VEA). For CERs, rs680379 in the SPTLC3 gene, which encodes a subunit of the rate-limiting enzyme in CER biosynthesis, associated with a range of species (e.g. CER[N(24)S(19)]; P = 4.82 × 10−27). We observed three novel associations between SNPs at the CD83, SGPP1 and DEGS1 loci, and plasma CER traits (P < 5 × 10−8). 2SMR in the CARDIoGRAMplusC4D cohorts (60 801 cases; 123 504 controls) and in the DIAGRAM cohort (26 488 cases; 83 964 controls), using the genetic instruments from our family-based GWAS, did not reveal association between genetically determined differences in CER levels and CVD or diabetes. Two of the novel GWAS loci, SGPP1 and DEGS1, suggested a casual association between CERs and a range of haematological phenotypes, through 2SMR in the UK Biobank, INTERVAL and UKBiLEVE cohorts (n = 110 000–350 000).

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Heritability and family-based GWAS analyses of the N-acyl ethanolamine and ceramide plasma lipidome

McGurk

Williams

Guo

et al. 2021

Human Molecular Genetics

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“…EPA and DHA were also able to prevent the polarisation of T cells and activation of the small Rho GTPases Rhoα and Rac1, both events required for migration to target/inflamed tissue [66]. EPA and DHA have also been shown to increase the infiltration of CD4 + and CD8 + T cells in UV irradiated human skin, potentially through changes in the network of cutaneous bioactive lipids [67].…”

Section: Activation and Differentiationmentioning

confidence: 98%

Fatty acids – from energy substrates to key regulators of cell survival, proliferation and effector function

Cucchi¹,

Camacho‐Muñoz²,

Certo³

et al. 2020

CST

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Recent advances in immunology and cancer research show that fatty acids, their metabolism and their sensing have a crucial role in the biology of many different cell types. Indeed, they are able to affect cellular behaviour with great implications for pathophysiology. Both the catabolic and anabolic pathways of fatty acids present us with a number of enzymes, receptors and agonists/antagonists that are potential therapeutic targets, some of which have already been successfully pursued. Fatty acids can affect the differentiation of immune cells, particularly T cells, as well as their activation and function, with important consequences for the balance between anti-and pro-inflammatory signals in immune diseases, such as rheumatoid arthritis, psoriasis, diabetes, obesity and cardiovascular conditions. In the context of cancer biology, fatty acids mainly provide substrates for energy production, which is of crucial importance to meet the energy demands of these highly proliferating cells. Fatty acids can also be involved in a broader transcriptional programme as they trigger signals necessary for tumorigenesis and can confer to cancer cells the ability to migrate and generate distant metastasis. For these reasons, the study of fatty acids represents a new research direction that can generate detailed insight and provide novel tools for the understanding of immune and cancer cell biology, and, more importantly, support the development of novel, efficient and fine-tuned clinical interventions. Here, we review the recent literature focusing on the involvement of fatty acids in the biology of immune cells, with emphasis on T cells, and cancer cells, from sensing and binding, to metabolism and downstream effects in cell signalling.

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“…Diet supplementation with fish oil or omega-3 has a beneficial effect on skin inflammation. In UV-irradiated skin, EPA altered the lipidome, diminishing the production of pro-inflammatory lipids, and DHA inhibited the migration of Langerhans cells [ 103 ]. DHA is a substrate for the generation of mediators, as maresins are also involved in tissue regeneration.…”

Section: Inflammation and Fatty Acidsmentioning

confidence: 99%

Mediterranean Diet: Lipids, Inflammation, and Malaria Infection

Silva

Moraes

Gonçalves-de-Albuquerque

2020

IJMS

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The Mediterranean diet (MedDiet) consists of consumption of vegetables and healthy oils and have beneficial effects on metabolic and inflammatory diseases. Our goal here is to discuss the role of fatty acid content in MedDiet, mostly omega-3, omega-6, and omega-9 on malaria. Malaria affects millions of people around the globe. The parasite Plasmodium causes the disease. The metabolic and inflammatory alterations in the severe forms have damaging consequences to the host. The lipid content in the MedDiet holds anti-inflammatory and pro-resolutive features in the host and have detrimental effects on the Plasmodium. The lipids from the diet impact the balance of pro- and anti-inflammation, thus, lipids intake from the diet is critical to parasite elimination and host tissue damage caused by an immune response. Herein, we go into the cellular and molecular mechanisms and targets of the MedDiet fatty acids in the host and the parasite, reviewing potential benefits of the MedDiet, on inflammation, malaria infection progression, and clinical outcome.

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Dynamics of the human skin mediator lipidome in response to dietary ω‐3 fatty acid supplementation

Cited by 29 publications

References 61 publications

Heritability and family-based GWAS analyses of the N-acyl ethanolamine and ceramide plasma lipidome

Heritability and family-based GWAS analyses of the N-acyl ethanolamine and ceramide plasma lipidome

Fatty acids – from energy substrates to key regulators of cell survival, proliferation and effector function

Mediterranean Diet: Lipids, Inflammation, and Malaria Infection

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