Dynamics of receptor-operated Ca2+ currents through TRPC channels controlled via the PI(4,5)P2-PLC signaling pathway

Mori, Masayuki; Itsuki, Kyohei; Häse, H.; Sawamura, Seishiro; Kurokawa, Tetsuji; Mori, Yasuo; Inoue, Ryuji

doi:10.3389/fphar.2015.00022

Cited by 25 publications

(19 citation statements)

References 38 publications

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“…Calcium ion uptake by cells is related to voltagedependent calcium ion channels (23), receptor-operated calcium ion channels (24) or calcium ion release from the endoplasmic reticulum (25) following activation of PI3K (26), serine-threonine kinase (27), phospholipase A2 and phospholipase C (24,28) or calcium ion/ calmodulin-dependent protein kinase IIA (29). We found that the vacuolation induced by rCAMP factor in RAW cells is inhibited by the PI3K inhibitors LY294002 and wortmannin.…”

Section: Discussionmentioning

confidence: 99%

Streptococcus pyogenes CAMP factor promotes calcium ion uptake in RAW264.7 cells

Kurosawa

Oda

Domon

et al. 2018

Microbiology and Immunology

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Streptococcus pyogenes is a bacterium that causes systemic diseases such as pharyngitis and toxic shock syndrome. S. pyogenes produces molecules that inhibit the function of the human immune system, thus allowing growth and spread of the pathogen in tissues. It is known that S. pyogenes CAMP factor induces vacuolation in macrophages; however, the mechanism remains unclear. In the current study, the mechanism by which CAMP factor induces vacuolation in macrophages was investigated. CAMP factor was found to induce calcium ion uptake in murine macrophage RAW264.7 cells. In addition, EDTA inhibited calcium ion uptake and vacuolation in the cells. The L-type voltage-dependent calcium ion channel blockers nifedipine and verapamil reduced vacuolation. Furthermore, the phosphoinositide 3-kinase inhibitors LY294002 and wortmannin also inhibited the vacuolation induced by CAMP factor. Fluorescent microscopy revealed that clathrin localized to the vacuoles. These results suggest that the vacuolation is related to calcium ion uptake by RAW264.7 cells via L-type voltage-dependent calcium ion channels. Therefore, it was concluded that the vacuoles induced by S. pyogenes CAMP factor in macrophages are clathrin-dependent endosomes induced by activation of the phosphoinositide 3-kinase signaling pathway through calcium ion uptake.

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Section: Discussionmentioning

confidence: 99%

Streptococcus pyogenes CAMP factor promotes calcium ion uptake in RAW264.7 cells

Kurosawa

Oda

Domon

et al. 2018

Microbiology and Immunology

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“…Among the TRP channels, the TRPC family was indeed a perfect candidate to form ROCE; TRPC2, 3, 6, and 7 can be activated by DAG, a potent lipid messenger produced from PIP 2 by PLC activation, and activation of TRPC4 and 5 was PLC-dependent [52]. In mouse airway epithelial cells, TRPV4 participated in ROCE instead of SOCE [27], further indicating the possible involvement of TRP channels in ROCE.…”

Section: Discussionmentioning

confidence: 99%

Regulation of Intracellular Calcium by Carbon Monoxide in Human Bronchial Epithelial Cells

Zhang¹,

Yip²,

Ko³

2017

Cell Physiol Biochem

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Background/Aims: Carbon monoxide (CO) is an important autocrine/paracrine messenger involved in a variety of physiological and pathological processes. This study aimed to investigate the regulatory role of CO released by CO-releasing molecule-2 (CORM-2) in a P2Y receptor-mediated calcium-signaling pathway in the human bronchial epithelial cell line, 16HBE14o-. Methods: Intracellular calcium ([Ca²⁺]_i) was measured by fura-2 microspectrofluorimetry. D-myo-inositol-1-phosphate (IP₁) levels and cGMP-dependent protein kinase activity (PKG) were also quantified. Results: The exogenous application of CORM-2 increased both intracellular Ca²⁺ and IP₁, which are inhibited by U73122, a phospholipase C (PLC) inhibitor. In contrast, the P2Y₂/P2Y₄ receptor-mediated intracellular Ca²⁺ release and influx induced by UTP were inhibited in the presence of CORM-2. However, CORM-2 did not affect the store-operated Ca²⁺ entry (SOCE) induced by thapsigargin (Tg). Moreover, the inhibitory effect of CORM-2 on UTP-induced calcium increase could be attenuated by a soluble guanylyl cyclase (sGC) inhibitor, 1H-[1,2,4]oxadiazolo[4,3,-a]quinoxalin-1-one (ODQ), or a Protein Kinase G (PKG) inhibitor, KT5823, suggesting the involvement of sGC/PKG signaling in this process. Conclusion: CORM-2 serves a dual role in modulating [Ca²⁺]_i in 16HBE14o- cells. Thus, CO released by CORM-2 may act as a regulator of calcium homeostasis in human airway epithelia. These findings help further elucidate the function of CO in many physiological and pathological conditions.

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“…We observed that blackcurrant juice inhibited the BaCl 2 -induced contraction of the ileum. Barium causes the depolarization of smooth muscle, which results in the opening of voltage-dependent calcium channels and the entry of calcium ions into cells [30].…”

Section: Discussionmentioning

confidence: 99%

“…High concentrations of potassium ions are known to cause tonic contraction of the smooth intestinal muscles, causing the opening of voltage-dependent L-type calcium channels, allowing the entry of calcium ions into the cell [30]. Therefore, substances that inhibit contractions induced by high K + concentrations are considered calcium channel blockers [31].…”

Section: Discussionmentioning

confidence: 99%

Antispasmodic Effect of Blackcurrant (<b><i>Ribes nigrum</i></b> L.) Juice and Its Potential Use as Functional Food in Gastrointestinal Disorders

et al. 2018

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Objective: The purpose of this study was to investigate the relaxative effects of blackcurrant juice on the gastrointestinal smooth muscle in vitro. Materials and Methods: Berries of the blackcurrant cultivar Ometa were used for the preparation of the juice used. The spasmolytic activity of blackcurrant juice was tested on rat ileum isolated from male Wistar rats by monitoring its influence on spontaneous contractions, as well as contractions induced by potassium chloride (KCl), barium chloride (BaCl₂), calcium chloride (CaCl₂), and acetylcholine (Ach). The results are expressed as the mean ± standard deviation obtained in 6 measurements and statistical significance was determined by the Student t test, with p < 0.05 taken as significant. Results: The blackcurrant cultivar Ometa significantly reduced the frequency and the amplitude of spontaneous contractions (57.94 ± 3.44%) and Ach-induced contractions (42.74 ± 5.36%; p < 0.05) of the isolated rat ileum. Cumulative concentrations (0.01–3 mg/mL) of the Ometa juice also reduced contractions of the isolated rat ileum stimulated by KCl (51.46 ± 6.87%), CaCl₂ (57.54 ± 6.47%), and BaCl₂ (58.54 ± 10.55%). The inhibitory effects of the juice were proportional to the applied concentration. Conclusion: The antispasmodic effect of Ometa cultivar shows that common gastrointestinal disorders could be treated by the functional food.

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Dynamics of receptor-operated Ca2+ currents through TRPC channels controlled via the PI(4,5)P2-PLC signaling pathway

Cited by 25 publications

References 38 publications

Streptococcus pyogenes CAMP factor promotes calcium ion uptake in RAW264.7 cells

Streptococcus pyogenes CAMP factor promotes calcium ion uptake in RAW264.7 cells

Regulation of Intracellular Calcium by Carbon Monoxide in Human Bronchial Epithelial Cells

Antispasmodic Effect of Blackcurrant (<b><i>Ribes nigrum</i></b> L.) Juice and Its Potential Use as Functional Food in Gastrointestinal Disorders

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