Dynamics of phagocytic activity of neutrophils in rats under oxidative stress and the action of liposomal preparation

An analysis of literary sources on issues of a property of the blood and hemostasis suggests that this problem is a significant consideration given to human medicine. There is ongoing research on the properties of blood and systems of hemostasis in female animals at the time of feeding the fetus, their impact on the indices of reproductive function and viability of the offspring remains beyond the attention of researchers. The goal of the work was to study the dynamics of haematological parameters in cows of Holstein Black-and-White breed during the dry period. The research was carried out on the cows in the dynamics of the physiological course of the dry period (from the 8th to the 9th months of calving). Hematologic studies of blood samples taken from the cows in the course of the dry period were carried out according to generally accepted methods. The results of our research indicated that deep-calving cows show a decrease in the number of red blood cells, an increase in the number of leukocytes, an increase in the oxygen capacity of the blood and an increase in the colour index. The haemoglobin content and energy potential increased, the number of lymphocytes and neutrophils with segmental nucleus increased, and the number of neutrophils with a stick-shaped nucleus decreased as well. Consequently, the characteristics features of homeostasis of deep-calving cows are: reducing the number of erythrocytes and increasing the number of leukocytes, increasing the oxygen capacity of the blood and increasing the color index, hemoglobin content and energy potential; an increasing the number of lymphocytes, segmental neutrophils, as well as reducing neutrophils with a stick-shaped nucleus. Further work will have a focus on determining the haematological parameters of high-yielding cows in the post-partum period for studying the overall resistance of the body, as well as the prediction and correction of obstetric pathology.

Section: результати та їх обговоренняunclassified

Haematological indicators in high-produced cows in dynamics of dry period

Koreyba

2019

“…Вони містять лізосомні ферменти і головна їх функція -фагоцитоз. Нейтрофіли мають здатність активно пересуватися в тканинах до вогнища запалення і фагоцитувати мікроорганізми та інші дрібні частинки (Khariv & Hutyi, 2017;Kysera et al, 2018).…”

Section: результати та їх обговоренняunclassified

Changes of hematological parameters of in blood in cats ill with microsporium

Martyniv¹,

Kisera²

2019

There has been a massive tendency for cats to be kept as pets in Ukraine in recent years. The frequency of their diseases has also increased at the same time. Cats most often come into the homes of people from the street, from volunteers, rarely from nurseries. Due to this, Doctors often receive cats ill for microsporia, which is caused by fungi of the genus Microsporum and is one of the most common anthropozoonous diseases. The treatment process is carried out by a complex method. Analysis of the recommendations of various authors on the treatment of microsporia indicates the lack of immunostimulants in the conduct of a complex of therapeutic and preventive measures. Hematological studies were performed in order to find out the immune reactivity of the cats' organism during microsporia. The research was conducted on clinically healthy and cats ill for microspores. The obtained results of research showed that in cats with microsporia changes in morphological composition of blood were characterized by signs of anemia, leukopenia and lymphocytopenia. Changes in the structure of neutrophils were found in the type of vacuum and toxigenic grains in cats ill for microsporia. The toxic grains of neutrophils occur inside the cell as a result of physicochemical changes in the protein structure of the cytoplasm. Such cells can not provide phagocytosis of foreign agents and thus reduce the immune activity of the organism in cats ill for microsporium. A marked change in the shape of erythrocytes, which is characteristic of anemia, that is, erythrocytes-octantocytes with corneas by Jolly inclusions. Jolly's bodies are the remnants of the nucleus that have survived in erythrocytes because of the broken destruction of the normoblast nucleus. The obtained results indicate that the course and manifestation of microsporia in cats affects the immune status of the organism.

“…Визначення кількісних характеристик показників крові є невід'ємною частиною клінічних досліджень і дає добре розуміння інтенсивності еритропоезу і морфофункціонального стану клітин крові в критичні періоди онтогенезу (Stojanovskij et al, 2012;Kolomіес, 2013;Hariv & Gutyj, 2017;Stojanovskij et al, 2018). Як зазаначає Тельцов Л.П.…”

Section: вступunclassified

Stressor disorders of morphological indicators of bloods of ducks in critical periods of ontogenesis

Krogh

Stoyanovsky

2019

The article presents data hematol ohichnyh performance blood of ducks in critical periods of postnatal ontogenesis 2, 14, 21, 45, 90, 150, and 240 days of life, which are accordingly related to the full use of the yolk, intensive development of the digestive system and muscular tissue, change of fluff to the primary feather, juvenile molt, the beginning of the oviposition, the formation of the reproductive system and glands of the internal secretion, the continuation of the oviposition and the molt, which is accompanied by the complete renewal of the fine and medium feathers. Blood plays an important role in maintaining homeostasis , and studies of hematological parameters make it possible to assess the intensity of redox processes in tissues and organs, and studies of hematological parameters make it possible to estimate the intensity of redox processes in tissues and organs, namely the need for oxygen in connection with the physico-chemical and biochemical processes that occur in the body in the process of growth and development. They are dynamic and change with different factors faster than performance. The purpose of the study was to determine the dynamics of morphological parameters of duck blood during the critical periods of postnatal ontogeny . Research subject clinically healthy young Beijing duck meat breed performance direction. Changes in hematological parameters of ducks' blood reflect a picture of the impact of critical periods of ontogeny on ducks, characterized by 2 days of life, the numerical values of erythrocytes (2.45 ± 0.11 T/l) and the concentration of hemoglobin (102.90 ± 3.51 g/l), but the value of hematocrit was in the lower levels of physiological norms. While the leukocyte count of 31.20 ± 1.76 was close to the 240-day age, indicating high bird immunity after hatching , more likely to come from the use of yolk. In the study bird on the 14th day recorded an increase in the number of erythrocytes and leukocytes by 10.6 and 14.7%, hematocrit by 10.3% against a decrease in hemoglobin concentration by 12.8% (P < 0.05). At 21 days, the number of erythrocytes and hemoglobin concentration decreased by 8.2%, while hematocrit and leukocyte values approached the initial state of the experiment, amounting to 26.45 ± 2.40%, 32.06 ± 2.93 G/L. The presence of basophils in this age period indicates the launch of a chain of immune responses, indicating the formation of the immune system according to A. Malyukin. (2010). At day 45, erythrocytes and hemoglobin concentrations were reduced by 10.2% and 11.3% (P < 0.05), compared to the baseline period. During this period, hematocrit increased by 12.7%, which indicated an increase in blood cells in the ducts. Results at 90 days indicated a decrease in erythrocyte count by 14.3% (P < 0.05) and hemoglobin concentration by 11.8% (P < 0.05), while leukocyte count and hematocrit increased by 26.1 and 22.1% (P < 0.05) compared with ducklings of 2 days of age. In ducklings at 150 days of age, the erythrocyte count and hemoglobin concentration were close to the baseline, and the leukocyte count and hematocrit were increased by 19.6 and 39.3%, respectively (P < 0.05). At 240 days of ducklings life, an increase in the oxygen-transport system of blood was established in the form of an increase in the number of erythrocytes by 35.1% (P < 0.01), hemoglobin concentration by 28.7% (P < 0.01), hematocrit value by 50, 8% (P < 0.01) compared to 2-days of ducklings life, and the number of leukocytes was close to baseline, indicating a decrease in the reactivity of the organism.