Dynamics of Southern rice black-streaked dwarf virus in Rice and Implication for Virus Acquisition

Abstract

A novel viral disease of rice caused by Southern rice black-streaked dwarf virus (SRBSDV) has spread throughout East and Southeast Asia since the mid-2000s. Outbreaks of this viral disease occur yearly in southern parts of Japan concurrently with overseas migration of the planthopper vector Sogatella furcifera from southern China during the rainy season (from late June to early July). We examined the dynamics (changes in titer and localization) of SRBSDV on rice using reverse-transcription real-time polymerase chain reaction and determined the relationship between virus titer in plants and virus acquisition by S. furcifera. Under a constant temperature of 27°C, a substantial increase of SRBSDV titer in the leaf sheath together with typical symptoms (stunted growth and twisting of leaf tips) was observed at 20 days after the end of a 7-day exposure of viruliferous S. furcifera. Approximately 40% of S. furcifera acquired SRBSDV through feeding for 5 days on rice plants that were infected following exposure to viruliferous vectors for 10 to 15 days. These results suggest that rice infected by S. furcifera can be a source of SRBSDV before the next generation of S. furcifera emerges.

Quantification of southern rice black streaked dwarf virus and rice black streaked dwarf virus in the organs of their vector and nonvector insect over time

“…But neither virus was detected in the ZLH bodies, suggesting that neither virus was able to accumulate in ZLH. Although detection of SRBSDV in WBPH as well SBPH and RBSDV in only SBPH agrees with previously reported results (Chen and Zhang, 2005;Lee et al, 2005;Zhou et al, 2008;Pu et al, 2012;Matsukura et al, 2013;Li et al, 2014). But quantitative results showed SRBSDV accumulation was significantly higher in WBPH than SBPH.…”

“…Southern rice black streaked dwarf virus (SRBSDV), a tentative member of genus Fijivirus, family Reoviridae, was first recorded in 2001 in Yangxi County, China , and has spread to southern China, Vietnam, and Japan via long-distance migration of the white-backed planthopper (WBPH, Sogatella furcifera Horvath) Xu et al, 2012;Li et al, 2012;Hoang et al, 2011;Cheng et al, 2013;He et al, 2013;Matsukura et al, 2013). Another reovirus, rice black streaked dwarf virus (RBSDV) was first discovered in 1941 in Japan, in the 1960s in China and Korea (Hibino, 1996;Chen and Zhang, 2005;Lee et al, 2005), but re-emerged in 1996 and then rapidly spread to the most of regions where Oryza sativa ssp.…”

Virus altered rice attractiveness to planthoppers is mediated by volatiles and related to virus titre and expression of defence and volatile-biosynthesis genes

“…This virus was first detected in Guangdong, China in 2001 as the causative agent of a new rice disease called southern rice black-streaked dwarf disease20. In 2011 and 2012, the disease-affected areas in China and Vietnam were 700,000 and 500,000 hectares, respectively2122, and the virus has also been observed in Japan23. SRBSDV is efficiently transmitted by WBPH in a circulative, propagative, and persistent manner, but cannot be transmitted by BPH or by eggs or rice seeds2024.…”

New Model for the Genesis and Maturation of Viroplasms Induced by Fijiviruses in Insect Vector Cells

“…SRBSDV, transmitted by WBPH vectors in a persistent-propagative manner, has spread rapidly throughout southern China, northern Vietnam, and western Japan (10,(13)(14)(15)(16)(17)(18). SRBSDV virions are double shelled, spherical, and 65 to 70 nm in diameter (13,17,18).…”