Dynamics of Gut Microbiota Recovery after Antibiotic Exposure in Young and Old Mice (A Pilot Study)

Laubitz, Daniel; Typpo, Katri; Midura-Kiela, Monica T.; Brown, Clairessa M.; Barberán, Albert; Kiela, Pawel R.

doi:10.3390/microorganisms9030647

Cited by 18 publications

(11 citation statements)

References 44 publications

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“…In the elderly, antibiotic exposure was linked to microbiota diversity loss ( Ng et al, 2019 ; Ramirez et al, 2020 ). Recently, the age factor was further demonstrated in a mouse model, where older mice with 10 days of antibiotics could not achieve gut bacteria recovery after 6 months and even at the end of the study ( Laubitz et al, 2021 ). These results revealed that age is a critical factor associated with gut microbiome recovery after antibiotic treatment.…”

Section: Discussionmentioning

confidence: 99%

Oral antibiotics perturbation on gut microbiota after prostate biopsy

Wang

Lau

et al. 2022

Front. Cell. Infect. Microbiol.

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IntroductionThe use of antibiotics may induce the changes in gut microbiota. Previous studies have shown conflicting results on whether the changed gut microbiota by antibiotics can be recovered. Our study aims to investigate whether the gut microbiota could be recovered after a single dose of oral co-amoxiclav before transrectal ultrasound-guided transperineal prostate biopsy (TPPBx) in 5 weeks’ time.MethodsFifteen patients with elevated serum prostate-specific antigen (PSA) were recruited to provide pre-antibiotic and post-antibiotic fecal samples. The V4 region of 16S rRNA was sequenced. Analysis was performed by QIIME2. Alpha- and beta-diversities were analyzed, as well as the differential enrichment by Linear discriminant analysis Effect Size (LEfSe) analysis.ResultsBoth the alpha- and beta-diversities of the pre- and post-antibiotic fecal samples were significantly different. Genera that are associated with alleviation of inflammation were enriched in the pre-antibiotic fecal samples, while the inflammation-associated genera were more enriched in the post-antibiotic fecal samples.ConclusionA single dose of oral co-amoxiclav before TPPBx could have led to a change of gut microbiota that cannot be recovered in 5 weeks' time. Microbiome studies on prostate cancer patients should be cautioned on the use of post-prostate biopsy fecal sampling. Further studies should be conducted for the impact on gut microbiome for TPPBx alone.

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Section: Discussionmentioning

confidence: 99%

Oral antibiotics perturbation on gut microbiota after prostate biopsy

Wang

Lau

et al. 2022

Front. Cell. Infect. Microbiol.

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“…The reads were processed, and data analyzed as we described previously. 32 , 33 Briefly, de-multiplexing was performed using idemp script ( https://github.com/yhwu/idemp ). Filtering, dereplication, sample inference, chimera identification, and merging of paired-end reads was done with a reference-free Divisive Amplicon Denoising Algorithm 2 (Dada2, version 1.18.0) 34 package in RStudio (version 1.3.1093 with R version 4.0.3).…”

Section: Methodsmentioning

confidence: 99%

Section: Microbiome Analysismentioning

confidence: 99%

Interactive Effect of Combined Intermittent and Sustained Hypoxia and High-Fat Diet on the Colonic Mucosal Microbiome and Host Gene Expression in Mice

Mashaqi¹,

Laubitz²,

Morales³

et al. 2022

NSS

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Purpose Gut dysbiosis can cause cardiometabolic disease. Gut dysbiosis can be independently caused by high-fat diet (HFD) and intermittent hypoxia (IH; characterizing obstructive sleep apnea), but the interactive effect of combined intermittent and sustained hypoxia (IH+SH) (characterizing obesity hypoventilation syndrome) and HFD on gut dysbiosis is unclear. We aimed to investigate the interactive effect of a combination of IH and SH and HFD on proximal colonic microbiota and colonic gene expression pattern. Methods Male mice (n=16) were randomly received four different combinations of diet (normal versus HFD) and oxygen conditions (normoxia versus IH+SH) for 4 weeks. Bacterial DNA and mucosal epithelial cell RNA from proximal colon were collected for analysis of adherent microbiome and host’s gene expression analysis. Results HFD during IH+SH (22.6 ± 5.73; SD) led to greater Firmicutes: Bacteroidetes ratio than HFD during normoxia (5.89 ± 1.19; p=0.029). HFD significantly decreased microbial diversity as compared to normal diet, but the addition of IH+SH to HFD mildly reversed such effects. When compared to HFD during normoxia, HFD with combination of IH+SH resulted in changes to host mucosal gene expression for apical junctional complexes and adhesion molecules. Specifically, when compared to HFD during normoxia, HFD during IH+SH led to upregulation of Claudin 2 and Syk (tight junction dysfunction and increased mucosal permeability), while the barrier promoting claudin 4 was downregulated. Conclusion HFD during combined IH and SH causes greater gut dysbiosis and potentially adverse changes in colonic epithelial transcriptome than HFD during normoxia. The latter changes are suggestive of impaired gut barrier function.

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“…The data were analyzed as described previously 57 . Briefly, the fastq files were demultiplexed with the idemp script ( https://github.com/yhwu/idemp ), filtering, dereplication, chimera identification, and merging of paired-end reads were performed with dada2 R package 58 .…”

Section: Methodsmentioning

confidence: 99%

Decreased NHE3 expression in colon cancer is associated with DNA damage, increased inflammation and tumor growth

Laubitz

Gurney

Midura-Kiela

et al. 2022

Sci Rep

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Dysregulation of intra- and extracellular pH in cancer contributes to extracellular matrix remodeling, favors cell migration, proliferation, and metastasis. Although the primary attention has been focused on the role of the ubiquitous Na+/H+ exchanger isoform NHE1, the role of NHE3, the predominant apical isoform in colonic surface epithelium in the pathogenesis of colon cancer has not been investigated. Here, we show that NHE3 mRNA expression is significantly reduced in colorectal cancer patients and that low NHE3 expression is associated with poorer survival. Deletion of NHE3 in ApcMin mice evaluated at 15 weeks of age (significant mortality was observed beyond this time) led to lower body weights, increased mucosal inflammation, increased colonic tumor numbers, evidence of enhanced DNA damage in tumor surface epithelium, and to significant alteration in the gut microbiota. In the absence of the inflammatory and microbial pressors, ca. 70% knockdown of NHE3 expression in SK-CO15 cells led to reduced intracellular pH, elevated apical pH, dramatic differences in their transcriptomic profile, increased susceptibility to DNA damage, increased proliferation, decreased apoptosis and reduced adhesion to extracellular matrix proteins. Our findings suggest that loss of NHE3 in the surface epithelium of colonic tumors has profound consequences for cancer progression and behavior.

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Dynamics of Gut Microbiota Recovery after Antibiotic Exposure in Young and Old Mice (A Pilot Study)

Cited by 18 publications

References 44 publications

Oral antibiotics perturbation on gut microbiota after prostate biopsy

Oral antibiotics perturbation on gut microbiota after prostate biopsy

Interactive Effect of Combined Intermittent and Sustained Hypoxia and High-Fat Diet on the Colonic Mucosal Microbiome and Host Gene Expression in Mice

Decreased NHE3 expression in colon cancer is associated with DNA damage, increased inflammation and tumor growth

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