Dynamics of Collagen Oxidation and Cross Linking in Regenerating and Irreversibly Infarcted Myocardium

Akam, Eman A.; Bergemann, David; Ridley, S.; To, Samantha; Borlotti, Andrea; Moon, Brianna F.; Ma, Hua; Zhou, Yirong; Aguirre, Aaron D.; Caravan, Peter; González‐Rosa, Juan Manuel; Sosnovik, David E.

doi:10.1101/2023.07.25.549713

Cited by 2 publications

(3 citation statements)

References 44 publications

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“…In contrast, the post‐injury scar in the heart in zebrafish disappears, and the heart regenerates (Poss et al., 2002 ). As the progression of the degradation‐resistant collagen is suppressed in zebrafish hearts 1 month after heart cryoinjury, the collagen cross‐linking process after myocardial infarction may differ between mammals and zebrafish (Akam et al., 2023 ).…”

Section: Elastic Components Of the Ventricular Myocardiummentioning

confidence: 99%

Comparative analysis of ventricular stiffness across species

Usui,

Hanashima,

Hashimoto

et al. 2024

Physiological Reports

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Investigating ventricular diastolic properties is crucial for understanding the physiological cardiac functions in organisms and unraveling the pathological mechanisms of cardiovascular disorders. Ventricular stiffness, a fundamental parameter that defines ventricular diastolic functions in chordates, is typically analyzed using the end‐diastolic pressure–volume relationship (EDPVR). However, comparing ventricular stiffness accurately across chambers of varying maximum volume capacities has been a long‐standing challenge. As one of the solutions to this problem, we propose calculating a relative ventricular stiffness index by applying an exponential approximation formula to the EDPVR plot data of the relationship between ventricular pressure and values of normalized ventricular volume by the ventricular weight. This article reviews the potential, utility, and limitations of using normalized EDPVR analysis in recent studies. Herein, we measured and ranked ventricular stiffness in differently sized and shaped chambers using ex vivo ventricular pressure‐volume analysis data from four animals: Wistar rats, red‐eared slider turtles, masu salmon, and cherry salmon. Furthermore, we have discussed the mechanical effects of intracellular and extracellular viscoelastic components, Titin (Connectin) filaments, collagens, physiological sarcomere length, and other factors that govern ventricular stiffness. Our review provides insights into the comparison of ventricular stiffness in different‐sized ventricles between heterologous and homologous species, including non‐model organisms.

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Section: Elastic Components Of the Ventricular Myocardiummentioning

confidence: 99%

Comparative analysis of ventricular stiffness across species

Usui,

Hanashima,

Hashimoto

et al. 2024

Physiological Reports

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show abstract

“…25 Recently, two other probes were reported to detect allysine by binding Lys Ald , with one being activatable using the aggregation-induced emission mechanism 26 and the other fluorescence always-on. 27 These examples represent the most known work on the fluorescence imaging of allysine.…”

mentioning

confidence: 99%

“…However, it remains unknown whether its fluorogenic response might be interfered by other aldehydes or ketones, which can also conjugate with the hydrazine group to inhibit the a-PeT effect . Recently, two other probes were reported to detect allysine by binding Lys Ald , with one being activatable using the aggregation-induced emission mechanism and the other fluorescence always-on . These examples represent the most known work on the fluorescence imaging of allysine.…”

mentioning

confidence: 99%

An Allysine-Conjugatable Probe for Fluorogenically Imaging Fibrosis

Zhuang,

Yin,

et al. 2024

Anal. Chem.

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Allysine, a pivotal biomarker in fibrogenesis, has prompted the development of various radioactive imaging probes. However, fluorogenic probes targeting allysine remain largely unexplored. Herein, by leveraging the equilibrium between the nonfluorescent spirocyclic and the fluorescent zwitterionic forms of rhodamine-cyanine hybrid fluorophores, we systematically fine-tuned the environmental sensitivity of this equilibrium toward the development of fluorogenic probes for fibrosis. The trick lies in modulating the nucleophilicity of the ortho-carboxyl group, which is terminated with a hydrazide group for allysine conjugation. Probe B2 was developed with this strategy, which featured an N-sulfonyl amide group and exhibited superior fibrosis-to-control imaging contrast. Initially presenting as nonfluorescent spirocyclic aggregates in aqueous solutions, B2 displayed a notable fluorogenic response upon conjugation with protein allysine through its hydrazide group, inducing deaggregation and switching to the fluorescent zwitterionic form. Probe B2 outperformed the traditional Masson stain in imaging contrast, achieving an about 260–2600-fold ratio for fibrosis-to-control detection depending on fibrosis severity. Furthermore, it demonstrated efficacy in evaluating antifibrosis drugs. Our results emphasize the potential of this fluorogenic probe as an alternative to conventional fibrosis detection methods. It emerges as a valuable tool for antifibrosis drug evaluation.

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Dynamics of Collagen Oxidation and Cross Linking in Regenerating and Irreversibly Infarcted Myocardium

Cited by 2 publications

References 44 publications

Comparative analysis of ventricular stiffness across species

Comparative analysis of ventricular stiffness across species

An Allysine-Conjugatable Probe for Fluorogenically Imaging Fibrosis

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