Dynamics of Changes in the Races and Virulence of Wheat Powdery Mildew in Hungary between 1971 and 1999

Szunics, L.; Vida, Gyula; Bedö, Z.; Švec, Miroslav

doi:10.1007/978-94-017-3674-9_48

Cited by 14 publications

(10 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, only a few powdery mildew resistance genes have been cloned, with the gene Pm3 and its multiple alleles being the best characterized (Yahiaoui et al ., ; Bhullar et al ., , ). Gene Pm8 , introduced from rye ( Secale cereale L.), is the most widely used resistance gene resource in wheat breeding, but the gene has been reported to be no longer so effective in many wheat‐growing areas (Szunics et al ., ). Recent research showed that Pm3 is an ortholog of Pm8 , and may suppress Pm8 ‐mediated powdery mildew resistance through the formation of a PM3/PM8 heteromeric protein complex (Hurni et al ., , ).…”

Section: Introductionmentioning

confidence: 97%

E3 ubiquitin ligase gene CMPG1–V from Haynaldia villosa L. contributes to powdery mildew resistance in common wheat (Triticum aestivum L.)

Zhu

Fei

et al. 2015

The Plant Journal

View full text Add to dashboard Cite

SUMMARYPowdery mildew is one of the most devastating wheat fungal diseases. A diploid wheat relative, Haynaldia villosa L., is highly resistant to powdery mildew, and its genetic resource of resistances, such as the Pm21 locus, is now widely used in wheat breeding. Here we report the cloning of a resistance gene from H. villosa, designated CMPG1-V, that encodes a U-box E3 ubiquitin ligase. Expression of the CMPG1-V gene was induced in the leaf and stem of H. villosa upon inoculation with Blumeria graminis f. sp. tritici (Bgt) fungus, and the presence of Pm21 is essential for its rapid induction of expression. CMPG1-V has conserved key residues for E3 ligase, and possesses E3 ligase activity in vitro and in vivo. CMPG1-V is localized in the nucleus, endoplasmic reticulum, plasma membrane and partially in trans-Golgi network/early endosome vesicles. Transgenic wheat over-expressing CMPG1-V showed improved broad-spectrum powdery mildew resistance at seedling and adult stages, associated with an increase in expression of salicylic acid-responsive genes, H 2 O 2 accumulation, and cell-wall protein cross-linking at the Bgt infection sites, and the expression of CMPG1-V in H. villosa was increased when treated with salicylic acid, abscisic acid and H 2 O 2 . These results indicate the involvement of E3 ligase in defense responses to Bgt fungus in wheat, particularly in broad-spectrum disease resistance, and suggest association of reactive oxidative species and the phytohormone pathway with CMPG1-V-mediated powdery mildew resistance.

show abstract

Section: Introductionmentioning

confidence: 97%

E3 ubiquitin ligase gene CMPG1–V from Haynaldia villosa L. contributes to powdery mildew resistance in common wheat (Triticum aestivum L.)

Zhu

Fei

et al. 2015

The Plant Journal

View full text Add to dashboard Cite

show abstract

“…This readily detectable marker will also enable breeders to remove genes that are no longer effective from their breeding lines. If they stop using the Pm3g resistance allele, the frequency of the matching virulence gene could decline, as mildew races are often short-lived (Szunics et al 2001).…”

Section: Discussionmentioning

confidence: 99%

Identification of a microsatellite marker associated with Pm3 resistance alleles to powdery mildew in wheat

Bougot¹,

Lemoine²,

Pavoine³

et al. 2002

Plant Breeding

View full text Add to dashboard Cite

The Pm3 resistance locus, located on chromosome 1A in wheat, confers race-specific resistance to the obligate biotrophic fungus Blumeria graminis (DC) E.O. Speer f. sp. tritici, the causal agent of powdery mildew. Several Pm3 alleles are still effective in controlling the disease in Europe. A genetic map was constructed to map the Pm3g allele in the recombinant inbred line progeny from the cross ÔRE9001Õ (susceptible) · ÔCourtotÕ (resistant). Two microsatellite markers were closely mapped to Pm3g. The PSP2999 marker, which cosegregates with this allele, was shown to detect the presence of the Pm3g resistance allele in other cultivars. A collection of 56 wheat cultivars or advanced lines carrying one Pm3 allele was used to assess the allelespecific amplification of the PSP2999 marker. The same amplification pattern was obtained for lines with Pm3a, Pm3b, Pm3e, Pm3f and Pm3g alleles. Twenty genotypes carrying Pm3d showed a specific amplification pattern. This marker allowed the detection of the Pm3d allele in highly resistant lines whose resistance gene combinations were unknown. It was concluded that PSP2999 is a useful marker to detect Pm3 alleles in parents and to manage them in breeding programmes.

show abstract

“…Foram considerados resistentes os genótipos que apresentaram reação até valor 2. O número de genes ou combinações de genes inefetivos por isolado variou entre um (Pm8), em 1997, e nove (Pm3a, Pm3c, Pm4a, Pm4b, Pm6, Pm8, Pm1+..., Pm2+Mld Hungria carrega esse gene (Szunics et al, 2001). No presente trabalho, em todas as amostras, foi constatada freqüência de virulência entre 87,5% e 100% a esse gene, o que descarta o uso dessa fonte isolada em programas de melhoramento.…”

Section: Methodsunclassified

“…Isolates with virulence to five genes alone or in combinations were more frequent in 1995 and 1997, to six in 1999, and to seven in 2000, probably (Linhares, 1988b(Linhares, , 1988cKohli, 1996;McIntosh, 1998;Niewoehner & Leath, 1998). Em anos normais, as perdas de rendimento de grãos de trigo decorrentes da doença alcançam de 5% a 8% (Szunics et al, 2001). Em Passo Fundo, RS, há registros de perdas entre 10% e 62% (Fernandes et al, 1988;Linhares, 1988b;Reis et al, 1997).…”

Section: Effectiveness Of Wheat Resistance Genes To Powdery Mildewmentioning

confidence: 99%

Efetividade de genes de resistência de trigo a oídio

Costamilan¹,

Linhares²

2002

Fitopatol. bras.

View full text Add to dashboard Cite

Levantamentos periódicos da freqüência de virulência do patógeno causador de oídio (Blumeria graminis f. sp. tritici) em trigo (Triticum aestivum)auxiliam na escolha de fontes de resistência para uso em cruzamentos. Este trabalho relata resultados de cinco anos de verificação de efetividade de genes de resistência para populações patogênicas de B. graminis f. sp. tritici oriundas do Brasil e do Chile. Amostras de oídio foram recebidas pela Embrapa Trigo, Passo Fundo, RS, em 1995, 1996, 1997, 1999 e 2000. Inoculou-se cada isolado monopustular em plantas de trigo da série diferencial para raças. Foram identificadas 90 combinações diferentes de genes efetivos e inefetivos para resistência. Em 1995 e 1997, a maioria dos isolados foram virulentos com relação aos genes Pm3a, Pm3c, Pm8, Pm1+... e PmD1; em 1999, para Pm3a, Pm3c, Pm4b, Pm8, PmD1 e PmD2; e, em 2000, para Pm3a, Pm3c, Pm4b, Pm6, Pm8, PmD1 e PmD2. Em todos os anos analisados, permaneceram efetivos a todos os isolados os genes Pm2 e Pm4a+.... O número de genes inefetivos por isolado variou entre um e nove, no total de 13 genes ou combinações de genes testados. Isolados apresentando virulência a cinco genes ou combinações foram mais freqüentes em 1995 e em 1997, a seis em 1999 e a sete em 2000, o que pode significar aumento na complexidade da composição racial do patógeno a cada ano.

show abstract

Dynamics of Changes in the Races and Virulence of Wheat Powdery Mildew in Hungary between 1971 and 1999

Cited by 14 publications

References 3 publications

E3 ubiquitin ligase gene CMPG1–V from Haynaldia villosa L. contributes to powdery mildew resistance in common wheat (Triticum aestivum L.)

E3 ubiquitin ligase gene CMPG1–V from Haynaldia villosa L. contributes to powdery mildew resistance in common wheat (Triticum aestivum L.)

Identification of a microsatellite marker associated with Pm3 resistance alleles to powdery mildew in wheat

Efetividade de genes de resistência de trigo a oídio

Contact Info

Product

Resources

About