Dynamics of biofilm formation during anaerobic digestion of organic waste

Langer, Susanne; Schropp, Daniel; Bengelsdorf, Frank R.; Othman, Maazuza; Kazda, Marian

doi:10.1016/j.anaerobe.2013.11.013

Cited by 50 publications

(29 citation statements)

References 25 publications

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“…Moreover, MacLeod and Daugulis () observed that certain hydrophobic bacteria formed aggregates in the aqueous phase instead of inside the NAP. Overall, cell aggregates offer an advantage to colonize a new habitat and to enhance the environmental quality of hostile habitats (Langer et al, ; Lazarova and Manem, ). In our particular case, the main factor likely promoting the formation of aggregates inside SO200 was the limitation in nutrients or water activity inside the NAP, since cell aggregation may have supported the preservation of the aqueous niches required for the growth of Methylosarcina .…”

Section: Resultsmentioning

confidence: 99%

Comparative performance evaluation of conventional and two‐phase hydrophobic stirred tank reactors for methane abatement: Mass transfer and biological considerations

Cantera

Estrada

Lebrero

et al. 2015

Biotech & Bioengineering

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This study demonstrated for the first time the capability of methanotrophs to grow inside silicone oil (SO200) and identified the optimum cultivation conditions for enrichment of hydrophobic methanotrophs (high dilution rates (D) and low CH4 transfer rates). The potential of the hydrophobic methanotrophs enriched was assessed in a single-phase stirred tank reactor (1P-STR) and in a two-phase stirred tank reactor (2P-STR). Different operational conditions were systematically evaluated in both reactors (SO200 fractions of 30 and 60 %, stirring rates of 250 and 500 rpm, and D of 0.1-0.35 day(-1) with and without biomass retention). The results showed that the TPPB only supported a superior CH4 abatement performance compared to the 1P-STR (40% enhancement at 250 rpm and 25% enhancement at 500 rpm) at a D of 0.3 day(-1) due to the retention of the biocatalytic activity inside the SO200, while the 1P-STR achieved higher elimination capacities (EC up to ≈3 times) than the TPPB under the rest of conditions tested (ECmax = 91.1 g m(-3) h(-1) ). Furthermore, the microscopic examination and DGGE-sequencing of the communities showed that the presence of SO200 influenced the microbial population structure, impacting on bacterial biodiversity and favoring the growth of methanotrophs such as Methylosarcina. Biotechnol. Bioeng. 2016;113: 1203-1212. © 2015 Wiley Periodicals, Inc.

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Section: Resultsmentioning

confidence: 99%

Comparative performance evaluation of conventional and two‐phase hydrophobic stirred tank reactors for methane abatement: Mass transfer and biological considerations

Cantera

Estrada

Lebrero

et al. 2015

Biotech & Bioengineering

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“…It has been reported that the placement of support materials within anaerobic reactors experience rapid attachment from mixed methanogenic consortia within a few hours [39,40], and can promote the early production of EPS [31].…”

Section: Discussionmentioning

confidence: 99%

Physicochemical analysis of initial adhesion and biofilm formation of Methanosarcina barkeri on polymer support material

Nguyen

Karunakaran

Collins

et al. 2016

Colloids and Surfaces B: Biointerfaces

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Article available under the terms of the CC-BY-NC-ND licence (https://creativecommons.org/licenses/by-nc-nd/4.0/). eprints@whiterose.ac.uk https://eprints.whiterose.ac.uk/ Reuse This article is distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs (CC BY-NC-ND) licence. This licence only allows you to download this work and share it with others as long as you credit the authors, but you can't change the article in any way or use it commercially. More information and the full terms of the licence here: https://creativecommons.org/licenses/ Takedown If you consider content in White Rose Research Online to be in breach of UK law, please notify us by emailing eprints@whiterose.ac.uk including the URL of the record and the reason for the withdrawal request. *Graphical Abstract HighlightsOf the six materials tested Methanosarcina barkeri selectively adheres to polytetrafluoroethylene (PTFE), polypropylene (PP) and polyvinyl chloride (PVC) The best support materials for initial adhesion after 2 h also performed best after 72 h. xDLVO model predictions correlated with experimental adhesion results. Applicability of the xDLVO model in identifying support materials for selective attachment of M. barkeri. ! *Highlights (for review) Physicochemical analysis of initial adhesion and biofilm formation of HighlightsOf the six materials tested Methanosarcina barkeri selectively adheres to polytetrafluoroethylene (PTFE), polypropylene (PP) and polyvinyl chloride (PVC) The best support materials for initial adhesion after 2 h also performed best after 72 h. xDLVO model predictions correlated with experimental adhesion results. Applicability of the xDLVO model in identifying support materials for selective attachment of M. barkeri. AbstractThe retention of selective biofilms of Methanosarcina species within anaerobic digesters could reduce start-up times and enhance the efficiency of the process in treating high-strength domestic sewage. The objective of the study was to examine the effect of the surface characteristics of six common polymer support materials on the initial adhesion of the model methanogen, Methanosarcina barkeri, and to assess the potential of these support materials as selective biofilm carriers. Results from both the initial adhesion tests and extended DLVO (xDLVO) model correlated with each other, with PVC (12% surface coverage/mm 2 ), PTFE (6% surface coverage/mm 2 ), and PP (6% surface coverage/mm 2 ), shown to be the better performing support materials for initial adhesion, as well as subsequent biofilm formation by M. barkeri after 72 h. These three support materials Experimental results showed that the type of material strongly influenced the extent of adhesion from M. barkeri (p < 0.0001), and the xDLVO model was able to explain the results in these environmental conditions. Therefore, DLVO physicochemical forces were found to be influential on the initial adhesion of M. barkeri. Scanning electron microscopy suggested that production of extracellular polymeric substances ...

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“…These experiments used similar substrates as described above as well as slaughter house waste material with an inoculum from the biogas plant described above. More details on the digestion experiments are given in [21].…”

Section: Specifications Of the Biogas Plants And Samplingmentioning

confidence: 99%

Fungi open new possibilities for anaerobic fermentation of organic residues

2014

Self Cite

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Background: Large amounts of fibre-rich organic waste material from public green and private gardens have to be treated environmentally friendly; however, this fibre-rich biomass has low biogas yields. This study investigated the presence of fungi in full-scale biogas plants as well as in laboratory reactors and elucidated the importance of fungi for the biogas process. Methods: The dominating members of the eukaryotic community were identified by analyzing 18S rRNA gene and internal transcribed spacer 1 (ITS1) region fragments of clone libraries. These identifications were accompanied by diverse microscopic techniques such as fluorescence microscopy and conventional scanning electron microscopy. Results: Cells of presumably fungal origin were characterized by intensive fluorescence and were about 1 order of magnitude larger than prokaryotic cells. Molecular techniques enabled to identify fungi from the subphyla Agaricomycotina, Mucoromycotina, Pezizomycotina, Pucciniomycotina and Saccharomycotina and from the class Neocallimastigomycetes. Members of these groups can be important for microbial degradation of complex compounds, due to the ability to penetrate cell walls, and thus open the cells for the influx of bacteria, further enhancing degradation.

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Dynamics of biofilm formation during anaerobic digestion of organic waste

Cited by 50 publications

References 25 publications

Comparative performance evaluation of conventional and two‐phase hydrophobic stirred tank reactors for methane abatement: Mass transfer and biological considerations

Comparative performance evaluation of conventional and two‐phase hydrophobic stirred tank reactors for methane abatement: Mass transfer and biological considerations

Physicochemical analysis of initial adhesion and biofilm formation of Methanosarcina barkeri on polymer support material

Fungi open new possibilities for anaerobic fermentation of organic residues

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