Dynamically Modified Capillary Columns for Liquid Chromatographic Separations of Biological Compounds

Abstract

Dynamically modified open tubular capillary columns were evaluated for separations of biological and clinical interest. Small diameter, dynamically modified columns were readily prepared by simple addition of cetyltrimethylammonium salts to the mobile phase. Separations similar to those obtained on conventional reversed-phase columns were observed for neutral solutes, and ion-pair reversed-phase separations were observed for charged solutes. Borosilicate and standard flint glasses were evaluated with respect to common etching procedures. A potential clinical application is presented in the separation of antitumor drugs.

Reverse micelle supercritical fluid chromatography

“…Although the solvent strength of liquid propane is much less than n-hexane (k'in propane was greater than 100 for all three analytes), the reverse micelle mobile phases of both solvents gave similar retention at 25 °C. This suggests the major effect is due to the presence of the reverse micelles or the surfactant (26,27).…”