Dynamic weight bearing is an efficient and predictable method for evaluation of arthritic nociception and its pathophysiological mechanisms in mice

Quadros, Andreza Urba de; Pinto, Lidiane de Castro; Fonseca, Miriam M.; Kusuda, Ricardo; Cunha, Fernando Q.; Cunha, Thiago M.

doi:10.1038/srep14648

Cited by 47 publications

(44 citation statements)

References 46 publications

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“…The presence of TNF in peripheral and central structures is fundamental for joint pain in arthritis (6,14,27,44). During joint inflammation in experimental models of arthritis, up-regulated TNF in the spinal cord is implicated in increased articular nociception (27,54). Quadros et al (27) demonstrated that challenging the joints of immunized mice with a high dose of mBSA produce marked articular hypernociception that is associated with increased TNF expression and microglial activation in the spinal cord.…”

Section: Discussionmentioning

confidence: 99%

“…TNF, a pro-nociceptive cytokine, is directly involved in various pain disorders, including joint pain in different forms of arthritis, due to central and peripheral actions (6,27). However, without conditional peripheral inflammation, TNF has not been shown to elicit persistent pain in arthritis.…”

Section: Prolonged Joint Nociception Is Dependent On Tnf In the Sensomentioning

confidence: 99%

“…The neutralization of TNF by anti-TNF drugs is an important pharmacological strategy for decreasing disease activity and joint pain (25,26). Experimentally, the neutralization of spinal TNF by intrathecal treatment with infliximab, an anti-TNF monoclonal antibody, decreased joint nociception in a mouse model of antigen-induced arthritis (AIA) (27). Moreover, the intraarticular injection of etanercept, a fusion protein based on the TNF receptor type 2 (TNFR2), reduces the responsivity of articular nociceptors to mechanical stimulation.…”

Section: Introductionmentioning

confidence: 99%

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Sensory Ganglia-Specific TNF Expression Is Associated With Persistent Nociception After Resolution of Inflammation

et al. 2020

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systemic injection of etanercept, an agent clinically utilized for TNF neutralization, at day 7 post arthritis induction, alleviated the persistent joint hyperalgesia by specific action in DRG. Our data suggest that neuroinflammation in DRG after the resolution of acute joint inflammation drives continuous neural sensitization resulting in persistent joint nociception in a TNF-dependent mechanism.

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Section: Discussionmentioning

confidence: 99%

Section: Prolonged Joint Nociception Is Dependent On Tnf In the Sensomentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sensory Ganglia-Specific TNF Expression Is Associated With Persistent Nociception After Resolution of Inflammation

et al. 2020

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“…This test reports parameters including weight, surface area, and time spent on each paw, along with the variability of these measures. The Dynamic Weight Bearing Test has been used in medial meniscus transection and adjuvant induced arthritis studies, finding reduced weight placed on the injured limb in both models 38, 44, 45 .…”

Section: Kinetic Gait Parametersmentioning

confidence: 99%

Gait analysis methods for rodent models of arthritic disorders: reviews and recommendations

Lakes

Allen

2016

Osteoarthritis and Cartilage

116

110

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Gait analysis is a useful tool to understand behavioral changes in preclinical arthritis models. While observational scoring and spatiotemporal gait parameters are the most widely performed gait analyses in rodents, commercially available systems can now provide quantitative assessments of spatiotemporal patterns. However, inconsistencies remain between testing platforms, and laboratories often select different gait pattern descriptors to report in the literature. Rodent gait can also be described through kinetic and kinematic analyses, but systems to analyze rodent kinetics and kinematics are typically custom made and often require sensitive, custom equipment. While the use of rodent gait analysis rapidly expands, it is important to remember that, while rodent gait analysis is a relatively modern behavioral assay, the study of quadrupedal gait is not new. Nearly all gait parameters are correlated, and a collection of gait parameters is needed to understand a compensatory gait pattern used by the animal. As such, a change in a single gait parameter is unlikely to tell the full biomechanical story; and to effectively use gait analysis, one must consider how multiple different parameters contribute to an altered gait pattern. The goal of this article is to review rodent gait analysis techniques and provide recommendations on how to use these technologies in rodent arthritis models, including discussions on the strengths and limitations of observational scoring, spatiotemporal, kinetic, and kinematic measures. Recognizing rodent gait analysis is an evolving tool, we also provide technical recommendations we hope will improve the utility of these analyses in the future.

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“…Mice were immunized as previously described (9,10,11). Briefly, the mice were sensitized with 500 ug of mBSA in 0.2 mL of an emulsion containing 0.1 mL of saline and 0.1 mL of Freund's Complete Adjuvant (CFA) (1 mg/mL of inactive Mycobacterium tuberculosis), administered by subcutaneous (s.c.) injection on day 0.…”

Section: Induction Of Antigen-induced Arthritis (Aia)mentioning

confidence: 99%

Clinical-like cryotherapy in acute arthritis of the knee improves inflammation signs, pain, joint swelling and motor performance in mice

Castro¹,

Machanocker²,

Perez³

et al. 2020

Preprint

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Background Assess the effect of clinical-like cryotherapy on inflammatory signs (in vivo neutrophil migration, cytokines and joint inflammation), pain, and joint swelling in mice with knee arthritis. Balance and motor coordination were also evaluated. Methods Young C57BL/6 mice were randomly divided into 3 groups (n = 10/group): control group: animals were not immunized with antigen-induced arthritis (AIA) and no intervention occurred; AIA group: animals immunized with methylated bovine serum albumin (mBSA) for knee arthritis; AIA + cryotherapy group: animals immunized with mBSA and submitted to cryotherapy. After 21days, the study was divided into 6-hour experimental analysis periods after injection-induced joint inflammation with mBSA (100 ug/joint) and a cryotherapy protocol was applied (ice pack, two 20-minute sessions). Number of synovial fluid neutrophils, cytokine levels, joint histology, pain, joint swelling and motor performance were also analyzed. Results Our results showed that the application of cryotherapy in mice with acute knee arthritis reduced inflammatory signs and improved the symptoms of pain and joint swelling, as well as motor coordination and balance in the animals. Conclusions Clinical-like cryotherapy used in the acute phase of arthritis reduced inflammatory signs and improved the symptoms of pain, joint swelling, motor coordination and balance in the mice.

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Dynamic weight bearing is an efficient and predictable method for evaluation of arthritic nociception and its pathophysiological mechanisms in mice

Cited by 47 publications

References 46 publications

Sensory Ganglia-Specific TNF Expression Is Associated With Persistent Nociception After Resolution of Inflammation

Sensory Ganglia-Specific TNF Expression Is Associated With Persistent Nociception After Resolution of Inflammation

Gait analysis methods for rodent models of arthritic disorders: reviews and recommendations

Clinical-like cryotherapy in acute arthritis of the knee improves inflammation signs, pain, joint swelling and motor performance in mice

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