Dynamic substrates for cell biology

Bugga, Pradeep; Mrksich, Milan

doi:10.1016/j.cocis.2018.09.003

Cited by 13 publications

(12 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Stimuli-responsive surfaces are a class of surface that can undergo pronounced changes in properties and functions, e.g. wettability, roughness, biointeractions, in response to an external stimulus or a change in the surrounding environment. , Such surfaces have attracted much attention in the biomedicine and biotechnology communities and have led to the concept of stimuli-responsive biointerfaces. , On the one hand, they provide a means of mimicking the dynamic interfacial molecular interactions involved in biological processes and are thereby useful in fundamental biological research. , On the other hand, they serve as tools for the modulation of biointerfacial interactions such as protein adsorption and cell adhesion and have great potential for practical applications including bioseparations, diagnostics, and cell culture. , In previous work on the design of stimuli-responsive biointerfaces, two approaches have been adopted: (i) modification with stimuli-responsive polymers (e.g., thermoresponsive poly( N -isopropylacrylamide) (PNIPAAm)) to endow surfaces with responsive properties, and (ii) introduction of chemical components (e.g., host–guest couples, dynamic covalent bonds, and metal coordination pairs) that participate in reversible chemical interactions to control the presentation of bioactive ligands. , …”

Section: Introductionmentioning

confidence: 99%

Multistimulus Responsive Biointerfaces with Switchable Bioadhesion and Surface Functions

Yang

Zheng

Wei

et al. 2020

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Stimuli-responsive biointerfaces can serve as dynamic tools for modulation of biointerfacial interactions. Considering the complexity of biological environments, surfaces with multistimulus responsive switchable bioactivity are of great interest. In the work reported herein, a multistimulus responsive biointerface with on–off switchable bioadhesion (protein adsorption, bacterial adhesion, and cell adhesion) and surface functions in response to change in temperature, pH, or sugar content is developed. This surface is based on a silicon modified with a copolymer containing a thermoresponsive component (poly(N-isopropylacrylamide)) and a component, phenylboronic acid, that can form pH-responsive and sugar-responsive dynamic boronate ester bonds with diol-containing molecules. It is shown that biointeractions including protein adsorption and release, bacteria and cell attachment and detachment on this surface can be regulated by changing temperature, pH, and sugar content of the medium, either individually or all three simultaneously. Furthermore, this surface can switch between two different functions, namely between killing and releasing bacteria, by introduction of a diol-containing biocidal compound. Compared to switchable surfaces that are responsive to only one stimulus, our multistimulus responsive surface is better adapted to respond to the multifunctional complexities of the biological environment and thus has potential for use in numerous biomedical and biotechnology applications.

show abstract

Section: Introductionmentioning

confidence: 99%

Multistimulus Responsive Biointerfaces with Switchable Bioadhesion and Surface Functions

Yang

Zheng

Wei

et al. 2020

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…For this purpose, thiol anchors are connected to the substrates that bind to gold surfaces. This principle was extensively demonstrated using hydroquinone electrogens by Mrksich and coworkers [ 78 , 79 ]. The authors observed that the rates of enzymatic hydrolysis of gold immobilized ester substrates can be measured in real time voltametrically with no adverse effect on the assay caused by immobilization.…”

Section: Classification Of Synthetic Enzyme Substratesmentioning

confidence: 99%

Modified Enzyme Substrates for the Detection of Bacteria: A Review

Pala¹,

Širec²,

Spitz³

2020

Molecules

View full text Add to dashboard Cite

The ability to detect, identify and quantify bacteria is crucial in clinical diagnostics, environmental testing, food security settings and in microbiology research. Recently, the threat of multidrug-resistant bacterial pathogens pushed the global scientific community to develop fast, reliable, specific and affordable methods to detect bacterial species. The use of synthetically modified enzyme substrates is a convenient approach to detect bacteria in a specific, economic and rapid manner. The method is based on the use of specific enzyme substrates for a given bacterial marker enzyme, conjugated to a signalogenic moiety. Following enzymatic reaction, the signalophor is released from the synthetic substrate, generating a specific and measurable signal. Several types of signalophors have been described and are defined by the type of signal they generate, such as chromogenic, fluorogenic, luminogenic, electrogenic and redox. Signalophors are further subdivided into groups based on their solubility in water, which is key in defining their application on solid or liquid media for bacterial culturing. This comprehensive review describes synthetic enzyme substrates and their applications for bacterial detection, showing their mechanism of action and their synthetic routes.

show abstract

“…Changes in these interactions as a consequence of the ECM remodeling give rise to specific cell signaling and intracellular cascades, consequently triggering relevant cell behaviors like adhesion, migration, proliferation, differentiation, and apoptosis . To mimic the above dynamic interactions in artificial matrices, sophisticated synthetic biointerfaces, which are capable to reversible display bioactive ligands and dynamic modulating specific cell-biomaterial interactions, have attracted increasing attentions in both fundamental cell biology and tissue engineering. − In addition, the controlled interactions of membrane receptors with ligands immobilized on a biomaterial are also relevant in molecular targeting and isolation methods in medical diagnostics and therapeutics. , …”

Section: Introductionmentioning

confidence: 99%

Dynamic Synthetic Biointerfaces: From Reversible Chemical Interactions to Tunable Biological Effects

Tian

Liu

et al. 2019

Acc. Chem. Res.

View full text Add to dashboard Cite

CONSPECTUS: Dynamic synthetic biointerface is a new concept of biomaterials with smart surface properties capable of controlled display of bioactive ligands, dynamic modulation of cell-biomaterial interactions, and subsequently clever manipulation of fundamental cell behaviors like adhesion, migration, proliferation, differentiation, apoptosis, and so on. As mimics of the extracellular matrix (ECM), such molecularly dynamic biointerfaces have attracted increasing attention because of their tunable biological effects with great significance in in situ cell biology, tissue engineering, drug targeting, and cell isolation for cancer theranostics. Approaches to control bioligand presentation on materials mainly rely on surface functionalization with dynamic or reversible chemical linkers to which the ligands are tethered. Photoelectric-transformable or photocleavable chemistry, host−guest supramolecular chemistry, and multiple noncovalent interactions were initially employed for fabrication of dynamic synthetic biointerfaces. However, the external stimuli required in these systems, including electrochemical potential, electrochemical reaction, and near-infrared or UV light, are mostly invasive to living cells; and few of them are able to respond to the stimuli occurring in natural biological processes. In addition, most of current systems focused only on the control of cell adhesion, other cell behaviors like migration, differentiation and apoptosis have rarely been explored. Therefore, the development of novel synthetic biointerfaces that permit access to noninvasive control of diverse cell behaviors still represents a key challenge in biomaterials science. Our group pioneers the use of reversible covalent bonds, metal coordinative interactions, and the molecular affinity of molecularly imprinted synthetic receptors as the dynamic driving forces for the fabrication of smart biointerfaces. Several typical biological stimuli, such as glycemic volatility, body temperature fluctuations, regional disparity of pH values, and specific biomolecules, were tactfully involved in our systems. In this Account, we highlight the strategies we have used on the exploitation of dynamic synthetic biointerfaces based on the above three types of reversible chemical interactions. While our attention has been focused on biologically stimuli-responsive or other noninvasive ligand presentation, the versatility of dynamic synthetic biointerfaces in control of cell adhesion, directing cell differentiation, and targeting cell apoptosis has also been successfully demonstrated. In addition, a paradigm shift of dynamic synthetic biointerfaces from macroscopic to microscopic scale (e.g., nanobiointerfaces) was conceptually demonstrated in our research. The potential applications of these developed dynamic systems, including fundamental cell biology, surface engineering of biomaterials, scaffold-free tissue engineering, cellbased cancer diagnosis, and drug targeting cancer therapy, were also introduced, respectively. Although the development of dyna...

show abstract

Dynamic substrates for cell biology

Cited by 13 publications

References 60 publications

Multistimulus Responsive Biointerfaces with Switchable Bioadhesion and Surface Functions

Multistimulus Responsive Biointerfaces with Switchable Bioadhesion and Surface Functions

Modified Enzyme Substrates for the Detection of Bacteria: A Review

Dynamic Synthetic Biointerfaces: From Reversible Chemical Interactions to Tunable Biological Effects

Contact Info

Product

Resources

About