Dynamic secretion during meiotic reentry integrates the function of the oocyte and cumulus cells

Çakmak, Hakan; Franciosi, Federica; Zamah, A.M.; Cedars, Marcelle I.; Conti, Marco

doi:10.1073/pnas.1519990113

Cited by 43 publications

(37 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The achievement of meiotic competency involves several biochemical and molecular changes (Cakmak et al . ). The successful completion of meiotic competency determines oocyte quality, which is a primary requirement for successful fertilization, early embryonic development and reproductive outcome (Liu et al .…”

Section: Discussionmentioning

confidence: 97%

Maturation promoting factor destabilization mediates human chorionic gonadotropin induced meiotic resumption in rat oocytes

Tiwari

Chaube

2017

Dev Growth Differ

View full text Add to dashboard Cite

Human chorionic gonadotropin (hCG) mimics the action of luteinizing hormone (LH) and triggers meiotic maturation and ovulation in mammals. The mechanism by which hCG triggers meiotic resumption in mammalian oocytes remains poorly understood. We aimed to find out the impact of hCG surge on morphological changes, adenosine 3',5'-cyclic monophosphate (cAMP), guanosine 3',5'-cyclic monophosphate (cGMP), cell division cycle 25B (Cdc25B), Wee1, early mitotic inhibitor 2 (Emi2), anaphase-promoting complex/cyclosome (APC/C), meiotic arrest deficient protein 2 (MAD2), phosphorylation status of cyclin-dependent kinase 1 (Cdk1), its activity and cyclin B1 expression levels during meiotic resumption from diplotene as well as metaphase-II (M-II) arrest in cumulus oocyte complexes (COCs). Our data suggest that hCG surge increased cyclic nucleotides level in encircling granulosa cells but decreased their level in oocyte. The reduced intraoocyte cyclic nucleotides level is associated with the decrease of Cdc25B, Thr161 phosphorylated Cdk1 and Emi2 expression levels. On the other hand, hCG surge increased Wee1, Thr14/Tyr15 phosphorylated Cdk1, APC/C as well as MAD2 expression levels. The elevated APC/C activity reduced cyclin B1 level. The changes in phosphorylation status of Cdk1 and reduced cyclin B1 level might have resulted in maturation promoting factor (MPF) destabilization. The destabilized MPF finally triggered resumption of meiosis from diplotene as well as M-II arrest in rat oocytes.

show abstract

Section: Discussionmentioning

confidence: 97%

Maturation promoting factor destabilization mediates human chorionic gonadotropin induced meiotic resumption in rat oocytes

Tiwari

Chaube

2017

Dev Growth Differ

View full text Add to dashboard Cite

show abstract

“…It is therefore crucial to dissect the molecular components of HFF in order to elucidate the mechanisms and regulatory processes involved in oocyte maturation. EXs are known to be essential carriers for signal transduction-mediated interaction between follicular somatic cell sand oocytes in order to promote oocyte maturation in HFF (Cakmak et al, 2016;Van Blerkom, Figure 7 The pathways of the top 20 entries. (A) Coordinate axis Y : Pathway-Term entry name; coordinate axis X: log10 (P-Value).…”

Section: Discussionmentioning

confidence: 99%

The expression of small RNAs in exosomes of follicular fluid altered in human polycystic ovarian syndrome

Hu¹,

Tang²,

Zeng³

et al. 2020

PeerJ

View full text Add to dashboard Cite

Polycystic ovary syndrome (PCOS) can cause reproductive disorders that may affect oocyte quality from punctured follicles in human follicular fluid (HFF). The non-coding RNA family includes micro RNA (miRNA), piwi-interacting RNA (piRNA) and transfer RNA (tRNA); these non-coding RNA transcripts play diverse functions and are implicated in a variety of diseases and health conditions, including infertility. In this study, to explore the role of HFF exosomes in PCOS, we extracted and sequenced RNA from HFF exosomes of PCOS patients and compared the analysis results with those of non-PCOS control group. The HFF exosomes were successfully isolated and characterized in a variety of ways. The sequencing results of the HFF exosomal RNA showed that about 6.6% of valid reads in the PCOS group and 8.6% in the non-PCOS group were successfully mapped to the human RNA database. Using a hierarchical clustering method, we found there were ten small RNA sequences whose expression was significantly different between the PCOS and non-PCOS groups. We chose six of them to predict target genes of interest for further GO analysis, and pathway analysis showed that the target genes are mainly involved in biosynthesis of amino acids, glycine, serine and glycosaminoglycan, as well as threonine metabolism. Therefore, the small RNA sequences contained in HFF EXs may play a key role in the mechanism that drives PCOS pathogenesis, and thereby can act as molecular biomarkers for PCOS diagnosis in the future.

show abstract

“…Recently, other OSFs have been identified. Oocyte interleukin-7 (IL-7) was found to possibly have a role in human oocyte maturation [225].…”

Section: Oocyte-secreted Growth Factors: Bmp15 and Gdf9mentioning

confidence: 99%

Luteinizing Hormone Action in Human Oocyte Maturation and Quality: Signaling Pathways, Regulation, and Clinical Impact

Arroyo

Kim²,

Yeh

2020

Reprod. Sci.

View full text Add to dashboard Cite

The ovarian follicle luteinizing hormone (LH) signaling molecules that regulate oocyte meiotic maturation have recently been identified. The LH signal reduces preovulatory follicle cyclic nucleotide levels which releases oocytes from the first meiotic arrest. In the ovarian follicle, the LH signal reduces cyclic nucleotide levels via the CNP/NPR2 system, the EGF/EGF receptor network, and follicle/oocyte gap junctions. In the oocyte, reduced cyclic nucleotide levels activate the maturation promoting factor (MPF). The activated MPF induces chromosome segregation and completion of the first and second meiotic divisions. The purpose of this paper is to present an overview of the current understanding of human LH signaling regulation of oocyte meiotic maturation by identifying and integrating the human studies on this topic. We found 89 human studies in the literature that identified 24 LH follicle/oocyte signaling proteins. These studies show that human oocyte meiotic maturation is regulated by the same proteins that regulate animal oocyte meiotic maturation. We also found that these LH signaling pathway molecules regulate human oocyte quality and subsequent embryo quality. Remarkably, in vitro maturation (IVM) prematuration culture (PMC) protocols that manipulate the LH signaling pathway improve human oocyte quality of cultured human oocytes. This knowledge has improved clinical human IVM efficiency which may become a routine alternative ART for some infertile patients.

show abstract

Dynamic secretion during meiotic reentry integrates the function of the oocyte and cumulus cells

Cited by 43 publications

References 31 publications

Maturation promoting factor destabilization mediates human chorionic gonadotropin induced meiotic resumption in rat oocytes

Maturation promoting factor destabilization mediates human chorionic gonadotropin induced meiotic resumption in rat oocytes

The expression of small RNAs in exosomes of follicular fluid altered in human polycystic ovarian syndrome

Luteinizing Hormone Action in Human Oocyte Maturation and Quality: Signaling Pathways, Regulation, and Clinical Impact

Contact Info

Product

Resources

About