Dynamic Pressure Measurements in the Evaluation of a Pancreaticolienal Portal Diversion Model in the Rat

Jaffe, V; Mathie, Robert T.

doi:10.1159/000129111

Cited by 3 publications

(2 citation statements)

References 5 publications

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“…Concomitantly to the wound healing, venovenous portosystemic shunts (Figs. 2, 5, 6) and lymphatic communications will develop (1,2,3,10,12,13,14,31,33). Table 1 Body weights, relative weights of liver, platelet counts, serum pratein levels, colloid osmotic pressure and ammonium levels (M±MAD).…”

Section: Discussionmentioning

confidence: 99%

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Partial Splenectomy, Transposition of the Spleen to the Abdominal Wall or Splenohepatoplasty in Portal Hypertensive Rats. Effects on Portal Venous Pressure and Homeostasis - Microscopical Appearance of the Transposed Spleen

Tröbs¹,

Bennek²

1998

Eur J Pediatr Surg

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Reduction of the splenic volume by partial resection and collateral development after transposition are of potential value in the elective treatment of esophageal varices, hypersplenism and ascites. A study was performed on young Wistar rats. A simple animal model of extrahepatic portal hypertension was used, narrowing the portal vein to an outer diameter of one millimeter (PVS). One day, three weeks and seven weeks after this operation the portal venous pressure was elevated as compared with the sham-operated controls. The portal hypertension was statistically significant at week three (1.31 +/- 0.04 vs. 0.72 +/- 0.18 kPa, p = 0.01). Portocaval pressure gradient after partial resection of the spleen (SR) and intramuscular transposition (IMTrans) was compared with the pressure gradient after graded portal vein stenosis. Three weeks after intramuscular transposition portocaval pressure gradient was reduced (1.46 +/- 0.38 vs. 1.74 +/- 0.13 kPa, n.s.). This data supports the hypothesis that the portal venous high-pressure compartment and the systemic venous low-pressure compartment are maintained after development of natural shunts to the systemic circulation. In the following experiment different types of splenic transposition were tested and compared to each other and a normal control group or to rats with protal vein stenosis (PVS), respectively. After PVS, the animals were reoperated, an SR was performed and the wound surface of the spleen was transposed into the left abdominal wall subcutaneously (SCTrans) or intramuscularly (IMTrans) or to the left liver lobe (splenohepatoplasty, SHP), respectively. After three weeks the animals underwent measurements of organ weights, collections of blood samples and the spleen was investigated histologically. Blood cell counts were nearly normal but total serum protein, albumin and the colloid osmotic pressure were slightly diminished or significantly reduced (COP in the groups PVS + SR + IMTrans or SCTrans, p < 0.05) compared to the controls. Differences to the group with portal vein stenosis (PVS) were not significant. Serum protein electrophoresis after splenic transposition revealed an unobtrusive distribution pattern. Animals after SHP had the best increase in weight and high protein levels, but splenohepatic collateral development seemed sparse. Differences of plasma ammonium levels were statistically not significant, but some animals had elevated levels after transposition. Morphometry of routine-stained spleen specimens showed an intact immunoarchitecture of the transposed spleens.

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Section: Discussionmentioning

confidence: 99%

“…Experimentally, the model of portosysternic diversion by subcutaneous transposition of the spleen was used to study portal circulation, collateral capacity after complete ligature of this vein or to evaluate the efficiency of this method for enhancing the survival rate after hepatectomy (3,12,13,18).…”

Section: Animalsmentioning

confidence: 99%

Partial Splenectomy, Transposition of the Spleen to the Abdominal Wall or Splenohepatoplasty in Portal Hypertensive Rats. Effects on Portal Venous Pressure and Homeostasis - Microscopical Appearance of the Transposed Spleen

Tröbs¹,

Bennek²

1998

Eur J Pediatr Surg

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Effects of splenohepatopexy and omentopexy in experimentally induced infrahepatic portal hypertension in rats

et al. 2004

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The purpose of this study was to determine whether splenohepatopexy (SHP) or omental transposition (OP) could reverse the portal hypertension (PH) induced by portal vein ligation (PVL). Fifty-eight Wistar rats divided into four groups: in group A (22 rats) PH was produced by calibrated PVL. In group B (8 rats), SHP was performed. Group C animals (13 rats) underwent SHP and PVL. In group D (15 rats) both PVL and OP were done. In all of the rats, the portal pressures (PP) were measured before and after the production of PVL. For SHP, small areas on the spleen and liver capsula were stripped off, and these two areas were placed together. In group D, an omentum patch was tied on the prepared liver surface. Twelve weeks after surgery, the PP were measured again and portal angiography was done. The liver, omentum and spleen were histopathologically examined. In all of the groups, the PP after PVL were significantly higher than before PVL. Twelve weeks after surgery, the PP of SHP and OP groups were significantly lower than those with PVL alone. Angiographic study showed many collaterals between transposed tissue and liver. Histopathologically, the collaterals of animals with SHP or OP were found to be well developed to allow sufficient flow for PP reduction. Both SHP and OP may reverse the increased PP in PVL model of PH. In the future; these methods may be alternative techniques for PP reduction in human beings as well.

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Intrahepatic portal occlusion by microspheres: a new model of portal hypertension in the rat.

Jaffe

Berndt

Mathie

1994

Gut

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.4 mm Hg increase from basal), which declined gradually to a steady state pressure of 13-3-15*1 mm Hg (4-0-5.0 mm Hg increase). There was no significant difference between pressure increases produced by microspheres of differing sizes. It is concluded that portal hypertension can be produced acutely by blocking portal radicles with microspheres. The maximum pressure achieved, however, is substantially less than that obtained by total portal vein occlusion (mean: 57 6 mm Hg). This suggests the existence of functional intrahepatic portal systemic shunts not previously described in the normal liver.

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Dynamic Pressure Measurements in the Evaluation of a Pancreaticolienal Portal Diversion Model in the Rat

Cited by 3 publications

References 5 publications

Partial Splenectomy, Transposition of the Spleen to the Abdominal Wall or Splenohepatoplasty in Portal Hypertensive Rats. Effects on Portal Venous Pressure and Homeostasis - Microscopical Appearance of the Transposed Spleen

Partial Splenectomy, Transposition of the Spleen to the Abdominal Wall or Splenohepatoplasty in Portal Hypertensive Rats. Effects on Portal Venous Pressure and Homeostasis - Microscopical Appearance of the Transposed Spleen

Effects of splenohepatopexy and omentopexy in experimentally induced infrahepatic portal hypertension in rats

Intrahepatic portal occlusion by microspheres: a new model of portal hypertension in the rat.

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