Dynamic multicuvette fluorometer-spectrophotometer based on the GeMSAEC fast analyzer principle

Tiffany, T. O.; Burtis, C. A.; Mailen, J.C.; Thacker, L. H.

doi:10.1021/ac60331a023

Cited by 25 publications

(6 citation statements)

References 15 publications

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“…The detection limit was estimated to be 7.7 x '10 (0.3 ppb) by the method of Gabriels {11). These detection limits fall within the reported range of 0.002 ppb to 1.7 ppb for new and sensitive instrumentation, some utilizing high intensity laser sources (2)(3)(4)(5)(6)(7).…”

Section: Resultssupporting

confidence: 75%

Optical stages for modifying a commercial spectrometer for fluorescence measurements

Bryant¹,

Hammons²,

Henderson³

1980

Anal. Chem.

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Section: Resultssupporting

confidence: 75%

Optical stages for modifying a commercial spectrometer for fluorescence measurements

Bryant¹,

Hammons²,

Henderson³

1980

Anal. Chem.

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“…The entire system is inter faced with a digital computer and teletype printer. Details of the equipment, procedures and princi ples have been previously published [10].…”

Section: Methodsmentioning

confidence: 99%

Detection of Hepatitis B Surface Antigen with the Miniature Centrifugal Fast Analyzer

Wenz¹,

Karmen²,

Feng³

1979

Vox Sanguinis

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A modified reversed passive hemagglutination test for the detection of hepatitis B surface antigen HBsAg is described. Sera and reagent cells coated with antibody to HBsAg (anti-HBs) are loaded separately into the rotor of a miniature centrifugal fast analyzer. The rotor is centrifuged briefly to transfer the components into its cuvettes. After mixing, the suspensions are allowed to stand at room temperature for 30 min, following which the rotor is again centrifuged and the absorbance of each cuvette is monitored. Cells suspended in serum containing HBsAg leave the light path more rapidly than cells suspended in sera free of antigen. The magnitude of change in absorbance varies directly with the concentration of the antigen. In 45 sera tested by the conventional V-plate technique, findings were as follows: 21 positive, 19 false positive and 5 negative. The automated procedure unequivocally differentiated the 21 positives; results for the false positive and negative specimens were identical and clearly distinguishable from the positive results. The automated procedure enhances specificity, offers equivalent sensitivity, and results that are quantitative and objective.

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“…The initial systems determined concentration by spectrophotometry. With the use of 900 and 1800 optics, it has been possible to expand the detection systems to include fluorometry and nephelometry (6,7). In addition, a rotor has been designed so that the cuvettes have their longitudinal dimension parallel to the light beam (8).…”

Section: General Clinical Chemistrymentioning

confidence: 99%

Instrumentation in Clinical Chemistry

Elin

1980

Science

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Dynamic multicuvette fluorometer-spectrophotometer based on the GeMSAEC fast analyzer principle

Cited by 25 publications

References 15 publications

Optical stages for modifying a commercial spectrometer for fluorescence measurements

Optical stages for modifying a commercial spectrometer for fluorescence measurements

Detection of Hepatitis B Surface Antigen with the Miniature Centrifugal Fast Analyzer

Instrumentation in Clinical Chemistry

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