Dynamic microwave-assisted extraction of flavonoids from Saussurea medusa Maxim cultured cells

Gao, Min; Song, Baozhen; Liu, Chun-Zhao

doi:10.1016/j.bej.2006.09.004

Cited by 96 publications

(52 citation statements)

References 17 publications

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“…Currently, there is growing concern for the development of different techniques, for example, supercritical-fluid extraction (SCFE), microwave assisted extraction (MAE), as well as pressurized solvent extraction (PSE), which are applicable for the recovery and isolation of various natural components such as lipids, steroids, terpenoids, phenolics and essential oils from plant materials (Kaufmann and Christen, 2002;Gao et al, 2006). In this way, enzyme-assisted cold pressing and enzyme-assisted aqueous extraction have emerged as recent eco-friendly technological developments (Latif and Anwar, 2009;.…”

Section: Soxhlet Extraction (N-hexane Extraction)mentioning

confidence: 99%

Enzyme-aided cold pressing of flaxseed (<i>Linum usitatissimum</i> L.): Enhancement in yield, quality and phenolics of the oil

Anwar¹,

Zreen²,

Sultana³

et al. 2013

Grasas y Aceites

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RESUMENPrensado en frío de semillas de lino (Linum usitatissimum L.) con enzimas asistida: Mejora en el rendimiento, la calidad y los compuestos fenólicos del aceite Se evalúa el efecto de diferentes preparaciones enzimá-ticas (Viscozyme L, Kemzyme y Feedzyme) sobre el rendimiento y propiedades fisicoquímicas y antioxidantes de aceites de lino prensados en frío. El rendimiento en aceite (35,2-38,0%) de las semillas de lino prensadas en frío (ETCPF), y tratadas con enzimas, aunque menor que el rendimiento mediante Soxhlet (SEO), fue considerablemente mayor en comparación con el control (32,5%), mientras que el contenido de proteína, fibra, y cenizas no se vieron afectados por el tratamiento enzimático. La mayoría de los pará-metros físico-químicos tales como el índice de refracción, densidad, índice de yodo, el contenido de ácidos grasos libres, índice de saponificación, el color y el perfil de ácidos grasos no variaron significativamente entre el aceite ETCPF, SEO y el control. Curiosamente, el estado de oxidación en términos de peróxidos, p-anisidina, dienos y trienos conjugados, período de inducción (método Rancimat), así como puntuación sensorial del aceite ETCPF fueron superiores en comparación con el control. Una cantidad sensiblemente superior de tocoferoles (350-400 mg kg -1 ) se determinó en el aceite ETCPF, en relación con el control (270 mg kg -1 ), mostrando un aumento de 22,8 a 32,5% en la recuperación de los tocoferoles totales. Por otra parte, el aceite de ETCPF mostró mayor actividad antioxidante y fenoles totales y contenido de ácidos fenólicos individuales. Este estudio aboga por la extracción mediante presión en frío con enzima asistida como una alternativa viable al prensado en frío convencional para mejorar no sólo el rendimiento de extracción sino también la calidad de los componentes funcionales de alto valor como los de los aceites de linaza. PALABRAS CLAVE: Ácidos fenólicos -Carbohidrasas -HPLC -Linolénico -Parámetros físico-químicos -Prensado en frío de aceite -Propiedades antioxidante -Tocoferoles -TPC. SUMMARY Enzyme-aided cold pressing of flaxseed (Linum usitatissimum L.): Enhancement in yield, quality and phenolics of the oilThe effect of different enzyme preparations (Viscozyme L, Kemzyme, and Feedzyme) on the yield and physicochemical and antioxidant properties of cold pressed flaxseed oil were assessed. The oil yield (35.2-38.0%) from enzyme-treated cold pressed flaxseeds (ETCPF), although lower than Soxhlet extracted oil (SEO) yield, was considerably higher when compared with the control (32.5%) while the contents of protein, fiber, and ash were unaffected by the enzymatic treatment. Most of the physicochemical parameters such as refractive index, density, iodine number, free fatty acid contents, saponification value, color and fatty acid profile did not vary significantly among the ETCPF oil, SEO and the control. Interestingly, the oxidation status in terms of peroxide value, para-anisidine value, conjugated dienes and triens and induction period (Rancimat method) as well as the...

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Section: Soxhlet Extraction (N-hexane Extraction)mentioning

confidence: 99%

Enzyme-aided cold pressing of flaxseed (<i>Linum usitatissimum</i> L.): Enhancement in yield, quality and phenolics of the oil

Anwar¹,

Zreen²,

Sultana³

et al. 2013

Grasas y Aceites

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“…Conventional extraction methods like solvent extraction, soxhlet extraction, and heat reflux extraction are time and solvent consuming, thermally unsafe, and involve risk of thermal decomposition of bioactive compounds. Soxhlet extraction and other conventional methods operate through cell permeation followed by solubilizing the active constituents by the extracting solvent [19]. In the last decade, there has been an increasing demand for new extraction techniques, amenable to automation, with shortened extraction times, and reduced organic solvent consumption-preventing pollution in analytical laboratories and reducing sample preparation costs [20,21].…”

Section: Introductionmentioning

confidence: 99%

Rapid extraction, isolation, and quantification of oleanolic acid fromLantana camaraL. Roots using microwave and HPLC-PDA techniques

Verma¹,

Jain²,

Nigam³

et al. 2013

Acta Chromatographica

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Summary. An ecofriendly solvent polarity based microwave-assisted extraction (MAE) technique was developed for the rapid extraction and isolation of bioactive oleanolic acid from roots of Lantana camara L. Several different influential extraction parameters such as microwave power, extraction time, solvent type, and volume were studied in a systematic fashion for the determination of optimum extraction conditions. Simply modified and rapid high-performance liquid chromatography-diode array detector (HPLC-DAD) method was also developed and validated for quantitative determination of oleanolic acid from roots of L. camara. Under optimum conditions, using a mixture of CHCl 3 :MeOH (60:40, v/v, 15 mL) as a solvent, 600 W microwave powers, and 50 °C temperature for 6 min of MAE produced a maximum yield of 1.23% (dry weight of roots). No degradation of the target analyte was observed at the optimum conditions as evidenced from the recovery studies performed with standard oleanolic acid. The proposed method also showed high degree of reproducibility; hence, it may be useful for maximum extraction and isolation of biologically active oleanolic acid.

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“…Closed-system microwave-assisted extraction (MAE) is an important technique for extracting valuable compounds from lignocellulosic materials (Guo et al 2001;Hao et al 2002;Gao et al 2006;Ghaly and Adams 2007;Hemwimon et al 2007;Ghani et al 2008;Yeoh et al 2008;Gujar et al 2009;Spigno and Faveri 2009;Michel et al 2011;. MAE of extractive substances may be affected by factors such as frequency and power of the microwave, time of applying microwave radiation, moisture content, particle size, ratio of solid to liquid, type and composition of solvent, temperature, pressure, and number of extraction cycles (Wang and Weller 2006;Yang and Zhang 2011;Hadkar et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Yield of Polyphenolic Substances Extracted From Spruce (Picea abies) Bark by Microwave-Assisted Extraction

Sládková¹,

Benedeková²,

Stopka³

et al. 2016

BioResources

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Closed-system microwave-assisted extraction was applied to extract total phenolics from spruce (Picea abies) bark, using 96.6% ethanol as an extractant. The influence of particle size (0.3; 1.0; 2.5 mm), time (3 to 20 min), and temperature (60; 80; 100 °C) on polyphenol recovery was also studied. Higher extraction temperature and smaller particle size resulted in a higher yield of extracted polyphenols. However, the effect of extraction time on yield was more complicated. The effect of all three factors is tentatively explained.

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Dynamic microwave-assisted extraction of flavonoids from Saussurea medusa Maxim cultured cells

Cited by 96 publications

References 17 publications

Enzyme-aided cold pressing of flaxseed (<i>Linum usitatissimum</i> L.): Enhancement in yield, quality and phenolics of the oil

Enzyme-aided cold pressing of flaxseed (<i>Linum usitatissimum</i> L.): Enhancement in yield, quality and phenolics of the oil

Rapid extraction, isolation, and quantification of oleanolic acid fromLantana camaraL. Roots using microwave and HPLC-PDA techniques

Yield of Polyphenolic Substances Extracted From Spruce (Picea abies) Bark by Microwave-Assisted Extraction

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