Dynamic Micropatterning of Cells on Nanostructured Surfaces Using a Cell-friendly Photoresist

Kweon, SoonHo; Song, Kwang Hoon; Park, Hyoungjun; Choi, Jung Min; Doh, Junsang

doi:10.1021/acsami.6b00318

Cited by 11 publications

(18 citation statements)

References 48 publications

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“…As is well-known, the ECM around the cells in the body is a dynamic 3D structure, and the cells live in the ecological environment [113]. The culture surfaces are used in many in vitro experiments only for the purpose of studying cell behavior on a static basis, and the properties of the substrate surface remain unchanged, which is incapable of reflecting the dynamic characteristics of ECM.…”

Section: Dynamic Materialsmentioning

confidence: 99%

Recent advance in surface modification for regulating cell adhesion and behaviors

Cai

Yang

et al. 2020

Nanotechnology Reviews

359

247

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Cell adhesion is a basic requirement for anchorage-dependent cells to survive on the matrix. It is the first step in a series of cell activities, such as cell diffusion, migration, proliferation, and differentiation. In vivo, cells are surrounded by extracellular matrix (ECM), whose physical and biochemical properties and micromorphology may affect and regulate the function and behavior of cells, causing cell reactions. Cell adhesion is also the basis of communication between cells and the external environment and plays an important role in tissue development. Therefore, the significance of studying cell adhesion in vitro has become increasingly prominent. For instance, in the field of tissue engineering and regenerative medicine, researchers have used artificial surfaces of different materials to simulate the properties of natural ECM, aiming to regulate the behavior of cell adhesion. Understanding the factors that affect cell behavior and how to control cell behavior, including cell adhesion, orientation, migration, and differentiation on artificial surfaces, is essential for materials and life sciences, such as advanced biomedical engineering and tissue engineering. This article reviews various factors affecting cell adhesion as well as the methods and materials often used in investigating cell adhesion.

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Section: Dynamic Materialsmentioning

confidence: 99%

Recent advance in surface modification for regulating cell adhesion and behaviors

Cai

Yang

et al. 2020

Nanotechnology Reviews

359

247

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“…To create surfaces that initially promote cell adhesion, but can trigger partial detachment of cells by light illumination, the surfaces of PDMP, a cell friendly photoresist polymer previously developed for dynamic cell micropatterning (Figure A), was modified. Original PDMP thin films have bioinert surfaces with minimal protein and cell attachment due to PEG side chains (a blue circle in Figure A) .…”

Section: Resultsmentioning

confidence: 99%

“…PDMP undergoes photochemical reaction in response to 365 nm wavelength of light illumination ( Figure A) and becomes soluble in buffer solution with pH ≈ 7.4, including phosphate buffered saline (PBS) and standard cell culture media. Thin films of PDMP immersed in PBS or cell culture media are spontaneously dissolved by several seconds of brief light illumination through a 4′,6‐diamidino‐2‐phenylindole (DAPI) filter of a standard fluorescence microscope, a widely used light source for live cell imaging for cell nucleus, with minimal cytotoxicity . Original PDMP thin films are cell repellent due to the presence of PEG side chains, thus by selectively removing PDMP thin films in certain regions by light illumination through a photomask, we can precisely control various dynamic cellular processes such as cell adhesion, spreading, and migration, and perform quantitative analysis .…”

Section: Introductionmentioning

confidence: 99%

“…Thin films of PDMP immersed in PBS or cell culture media are spontaneously dissolved by several seconds of brief light illumination through a 4′,6‐diamidino‐2‐phenylindole (DAPI) filter of a standard fluorescence microscope, a widely used light source for live cell imaging for cell nucleus, with minimal cytotoxicity . Original PDMP thin films are cell repellent due to the presence of PEG side chains, thus by selectively removing PDMP thin films in certain regions by light illumination through a photomask, we can precisely control various dynamic cellular processes such as cell adhesion, spreading, and migration, and perform quantitative analysis . However, the previous methods primarily focused on triggering cell spreading and migration by removing PDMP thin films adjacent to cells to form new adhesion due to the cell repellent nature of PDMP.…”

Section: Introductionmentioning

confidence: 99%

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In Situ Subcellular Detachment of Cells Using a Cell‐Friendly Photoresist and Spatially Modulated Light

et al. 2019

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Dynamic adhesion and detachment of subcellular regions occur during cell migration, thus a technique allowing precise control of subcellular detachment of cells will be useful for cell migration study. Previous methods for cell detachment were developed either for harvesting cells or cell sheets attached on surfaces with low resolution patterning capability, or for detaching subcellular regions located on predefined electrodes. In this paper, a method that allows in situ subcellular detachment of cells with ≈1.5 µm critical feature size while observing cells under a fluorescence microscope is introduced using a cell‐friendly photoresist and spatially modulated light. Using this method, a single cell, regions in cell sheets, and a single focal adhesion complex within a cell are successfully detached. Furthermore, different subcellular regions of migrating cells are detached and changes in cell polarity and migration direction are quantitatively analyzed. This method will be useful for many applications in cell detachment, in particular when subcellular resolution is required.

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“…Furthermore, a method for dynamic cell micropatterning on nanostructured surfaces was developed with a cell-friendly photoresist [ 20 ]. A device with different topographies was fabricated, and it showed how cells respond to distinct topographies during spreading.…”

Section: Microtechnologies For Cell Microenvironment Controlmentioning

confidence: 99%

Microtechnologies for Cell Microenvironment Control and Monitoring

et al. 2017

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A great breadth of questions remains in cellular biology. Some questions cannot be answered using traditional analytical techniques and so demand the development of new tools for research. In the near future, the development of highly integrated microfluidic analytical platforms will enable the acquisition of unknown biological data. These microfluidic systems must allow cell culture under controlled microenvironment and high throughput analysis. For this purpose, the integration of a variable number of newly developed micro- and nano-technologies, which enable control of topography and surface chemistry, soluble factors, mechanical forces and cell–cell contacts, as well as technology for monitoring cell phenotype and genotype with high spatial and temporal resolution will be necessary. These multifunctional devices must be accompanied by appropriate data analysis and management of the expected large datasets generated. The knowledge gained with these platforms has the potential to improve predictive models of the behavior of cells, impacting directly in better therapies for disease treatment. In this review, we give an overview of the microtechnology toolbox available for the design of high throughput microfluidic platforms for cell analysis. We discuss current microtechnologies for cell microenvironment control, different methodologies to create large arrays of cellular systems and finally techniques for monitoring cells in microfluidic devices.

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Dynamic Micropatterning of Cells on Nanostructured Surfaces Using a Cell-friendly Photoresist

Cited by 11 publications

References 48 publications

Recent advance in surface modification for regulating cell adhesion and behaviors

Recent advance in surface modification for regulating cell adhesion and behaviors

In Situ Subcellular Detachment of Cells Using a Cell‐Friendly Photoresist and Spatially Modulated Light

Microtechnologies for Cell Microenvironment Control and Monitoring

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