Dynamic adhesion and detachment of subcellular regions occur during cell migration, thus a technique allowing precise control of subcellular detachment of cells will be useful for cell migration study. Previous methods for cell detachment were developed either for harvesting cells or cell sheets attached on surfaces with low resolution patterning capability, or for detaching subcellular regions located on predefined electrodes. In this paper, a method that allows in situ subcellular detachment of cells with ≈1.5 µm critical feature size while observing cells under a fluorescence microscope is introduced using a cell‐friendly photoresist and spatially modulated light. Using this method, a single cell, regions in cell sheets, and a single focal adhesion complex within a cell are successfully detached. Furthermore, different subcellular regions of migrating cells are detached and changes in cell polarity and migration direction are quantitatively analyzed. This method will be useful for many applications in cell detachment, in particular when subcellular resolution is required.
Recently, with the requirements of several mobile products coming to the fore, to cope with the market needs, various mobile storage applications have been developed. Moreover, the core components and technologies for mobile optical disc drives have been under continuous development. In the case of optical storage, a small optical pickup is required to realize a mobile optical disc drive, because the optical parts and an actuator are the main factors determining the optical pickup height. In this paper, we examine the 5-mm-high mobile small form factor optical (SFO) drive and achieve a good readout signal with 6.8% jitter using a limit equalizer. In addition, the use of 2.3-mm-high actuator with a horizontally arranged magnetic circuit for the mobile pick up reduces the height of optical pickup, and leads to high DC and AC sensitivity for low power consumption.
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