Dynamic metabolic and transcriptomic profiling reveal synthetic characters and regulators of flavonoid biosynthesis in Camellia oleifera seeds

Song, Qiling; Ji, Ke; Yu, Xinran; Chen, Le; Wang, Linkai; Gong, Wenfang; Yuan, Deyi

doi:10.1016/j.indcrop.2022.115295

Cited by 24 publications

(12 citation statements)

References 70 publications

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“…14 structural genes and 5 TF genes in Actinidia arguta were selected, which involved in the flavonoid biosynthesis pathway [ 32 ]. TFs such as bHLH3, bHLH18, MYC2, and MYB44, were involved in the MeJA-mediated biosynthesis of flavonoids in C. oleifera seeds [ 21 ]. However, there are few studies on the genes involved in flavonoid biosynthesis and regulation in C. vietnamensis .…”

Section: Discussionmentioning

confidence: 99%

“…In addition to structural genes, TFs are important in flavonoid biosynthesis [ 37 ]. Three modules responsible for flavonoid accumulation were identified by WGCNA, in which TFs such as bHLH3, bHLH18, MYB44, MYB86, WRKY26, and WRKY32 were predicted to participate in flavonoid biosynthesis [ 21 ]. Eight MYBs (EpMYB108, EpMYB111, EpMYB84, EpMYB85, EpMYB78, EpMYB115, EpMYB86, and EpMYB76) were selected which related to anthocyanin biosynthesis [ 6 ].…”

Section: Discussionmentioning

confidence: 99%

“…At present, multiomics combined analysis has greater advantages in comprehensively explaining the regulatory mechanisms of plant stress responses at different molecular levels by associating the changes in metabolites with gene expression [ 17 , 18 ]. Many scholars have conducted preliminary investigations into flavonoids synthesis pathways of a variety of plants by transcriptome and the metabolome, such as Entada phaseoloides [ 6 ], Camellia sinensis [ 19 , 20 ], Eutrema salsugineum [ 8 ], Camellia oleifera [ 21 ], and so on. As a well-known exogenous inducing factor, MeJA is widely employed to produce bioactive compounds and can affect secondary metabolite content is now a hot spot of research and has been largely validated on other species, such as C. sinensis [ 19 ] and Carthamus tinctorius L. [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

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Integrative Metabolome and Transcriptome Analysis Reveals the Regulatory Network of Flavonoid Biosynthesis in Response to MeJA in Camelliavietnamensis Huang

Yan

Zheng

Wang

et al. 2022

IJMS

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Flavonoids are secondary metabolites widely found in plants, which perform various biological activities, such as antiinflammation, antioxidation, antitumor, and so on. Camellia vietnamensis Huang, a species of oil-tea Camellia tree, is an important woody oil crop species widely planted on Hainan Island, which provides health benefits with its high antioxidant activity and abundant flavonoid content. However, very little is known about the overall molecular mechanism of flavonoid biosynthesis in C. vietnamensis Huang. In this study, methyl jasmonate (MeJA) is used as an inducer to change the content of secondary metabolites in C. vietnamensis. Then, the potential mechanisms of flavonoid biosynthesis in C. vietnamensis leaves in response to MeJA were analyzed by metabolomics and transcriptomics (RNA sequencing). The results showed that metabolome analysis detected 104 flavonoids and 74 fatty acyls which showed different expression patterns (increased or decreased expression). It was discovered by KEGG analysis that three differentially accumulated metabolites (cinnamaldehyde, kaempferol and quercitrin) were annotated in the phenylpropanoid biosynthesis (ko00940), flavonoid biosynthesis (ko00941), and flavone and flavonol biosynthesis (ko00944) pathways. In the transcriptome analysis, 35 different genes involved in the synthesis of flavonoids were identified by MapMan analysis. The key genes (PAL, 4CL, CCR, CHI, CHS, C4H, FLS) that might be involved in the formation of flavonoid were highly expressed after 2 h of MeJA treatment. This study provides new insights and data supporting the molecular mechanism underlying the metabolism and synthesis of flavonoids in C. vietnamensis under MeJA treatment.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Integrative Metabolome and Transcriptome Analysis Reveals the Regulatory Network of Flavonoid Biosynthesis in Response to MeJA in Camelliavietnamensis Huang

Yan

Zheng

Wang

et al. 2022

IJMS

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“…C. oleifera is one of the most valuable economic woody crops and medical plant grown in Asia and has been cultivated for more than 2300 years [ 8 ]. The main profit driver of growing C. oleifera is to obtain the C. oleifera seeds and the camellia oil with a highly economical value.…”

Section: Introductionmentioning

confidence: 99%

Kaempferitrin: A Flavonoid Marker to Distinguish Camellia oleifera Honey

Huang

Zheng

et al. 2023

Nutrients

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C. oleifera is an economically important oilseed crop and medical plant. However, as a characteristic honey resource, the standard protocol used to identify the composition of C. oleifera honey has not been established yet. Previously, distinctive flavonoid has been shown as an effective marker to trace the botanical origin of honey. In this study, we examined the flavonoid types in C. oleifera honey and nine other monofloral honeys by using liquid chromatography tandem-mass spectrometry (LC-MS/MS) and compared the differences and identified eight distinct flavonoids in C. oleifera honey. Then, comparing the 8 flavonoids with the 14 flavonoids common to C. oleifera honey and nectar, two distinct flavonoids were identified in C. oleifera honey and nectar. Finally, we identified kaempferitrin as the distinct flavonoid marker in C. oleifera honey using the degree of influence of the partial least-squares discriminant analysis (PLS-DA) model on C. oleifera honey and ployfloral honey.

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“…Camellia oleifera Abel. (C. oleifera), a member of the genus Camellia in the tea family (Theaceae), has been cultivated in China for more than 2300 years (Wu et al, 2020;Song et al, 2022). As one of the world's four major woody oil trees, together with oil palm, olive and coconut, it has been widely used as edible oil, lubricant, and cosmetics (Gong et al, 2020;Lu et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Enhancing the accumulation of linoleic acid and α-linolenic acid through the pre-harvest ethylene treatment in Camellia oleifera

Ma²,

Wang³

et al. 2023

Front. Plant Sci.

Self Cite

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Camellia oleifera Abel. (C. oleifera) is an important woody edible oil tree species in China. The quality of C. oleifera oil (tea oil) is mainly determined by the contents of linoleic acid (LA) and α-linolenic acid (ALA). However, how to increase the contents of LA and ALA in tea oil and the corresponding regulating mechanism have not been clarified. In the present study, we found that the LA and ALA contents in C. oleifera seeds were significant positively associated with the concentrations of ethephon and were decreased by ethylene inhibitor treatment. Furthermore, 1.5 g L-1 ethephon could receive an optimal LA and ALA contents without adverse effects to the growth of ‘Huashuo’ trees in this study. The ethephon treatment also increased the contents of 1-aminocyclopropane-1-carboxylic acid (ACC), sucrose, soluble sugar and reducing sugar contents in seeds. Transcriptome analysis further suggested that exogenous ethephon application enhanced the accumulation of LA and ALA via regulating genes involved in LA and ALA metabolism, plant hormone signal transduction pathways, and starch and sucrose metabolism. Our findings confirm the role of ethylene in LA and ALA regulation and provide new insights into the potential utilization of ethylene as a LA and ALA inducer in C. oleifera cultivation.

show abstract

Dynamic metabolic and transcriptomic profiling reveal synthetic characters and regulators of flavonoid biosynthesis in Camellia oleifera seeds

Cited by 24 publications

References 70 publications

Integrative Metabolome and Transcriptome Analysis Reveals the Regulatory Network of Flavonoid Biosynthesis in Response to MeJA in Camelliavietnamensis Huang

Integrative Metabolome and Transcriptome Analysis Reveals the Regulatory Network of Flavonoid Biosynthesis in Response to MeJA in Camelliavietnamensis Huang

Kaempferitrin: A Flavonoid Marker to Distinguish Camellia oleifera Honey

Enhancing the accumulation of linoleic acid and α-linolenic acid through the pre-harvest ethylene treatment in Camellia oleifera

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