Integrative Metabolome and Transcriptome Analysis Reveals the Regulatory Network of Flavonoid Biosynthesis in Response to MeJA in Camelliavietnamensis Huang

Abstract

Flavonoids are secondary metabolites widely found in plants, which perform various biological activities, such as antiinflammation, antioxidation, antitumor, and so on. Camellia vietnamensis Huang, a species of oil-tea Camellia tree, is an important woody oil crop species widely planted on Hainan Island, which provides health benefits with its high antioxidant activity and abundant flavonoid content. However, very little is known about the overall molecular mechanism of flavonoid biosynthesis in C. vietnamensis Huang. In this study, methyl jasmonate (MeJA) is used as an inducer to change the content of secondary metabolites in C. vietnamensis. Then, the potential mechanisms of flavonoid biosynthesis in C. vietnamensis leaves in response to MeJA were analyzed by metabolomics and transcriptomics (RNA sequencing). The results showed that metabolome analysis detected 104 flavonoids and 74 fatty acyls which showed different expression patterns (increased or decreased expression). It was discovered by KEGG analysis that three differentially accumulated metabolites (cinnamaldehyde, kaempferol and quercitrin) were annotated in the phenylpropanoid biosynthesis (ko00940), flavonoid biosynthesis (ko00941), and flavone and flavonol biosynthesis (ko00944) pathways. In the transcriptome analysis, 35 different genes involved in the synthesis of flavonoids were identified by MapMan analysis. The key genes (PAL, 4CL, CCR, CHI, CHS, C4H, FLS) that might be involved in the formation of flavonoid were highly expressed after 2 h of MeJA treatment. This study provides new insights and data supporting the molecular mechanism underlying the metabolism and synthesis of flavonoids in C. vietnamensis under MeJA treatment.

Integrated Analysis of Widely Targeted Metabolomics and Transcriptomics Reveals the Effects of Transcription Factor NOR-like1 on Alkaloids, Phenolic Acids, and Flavonoids in Tomato at Different Ripening Stages

“…The genes associated with flavonoid biosynthesis are mainly classified into structural and regulatory genes [41]for instance, CHS, FLS, F3H, F3 H, C4H, etc. C4H plays an important role in flavonoid biosynthesis [42][43][44]. Correlation analysis showed that C4H at all three ripening stages positively regulated flavonoids including luteolin, hesperetin, homoeriodictyol, phloretin, butin, aromadendrin, naringenin chalcone, and pinobanksin.…”

Assessment of the Genetic Relationship and Population Structure in Oil-Tea Camellia Species Using Simple Sequence Repeat (SSR) Markers

“…Ninety-six pairs of SSR primers were selected for pre-screening based on the transcriptome data of C. vietnamensis (NCBI accession number: PRJNA825399) [ 24 ], in which 15 fluorescently labeled SSR primers were selected for further research ( Table 2 ). In addition, nine pairs of primers with good polymorphism were screened, referring to Song’s study ( Table 2 ) [ 25 ].…”

Induction of ferroptosis by natural products in non-small cell lung cancer: a comprehensive systematic review

“…Firstly, most studies have only explored the molecular mechanisms by which natural product from traditional Chinese medicine induces ferroptosis through a single pathway. Nevertheless, traditional Chinese medicine formulas and patent medicines widely used in clinical practice possess the characteristics of “multiple components, multiple targets, and multiple effects” ( Yan et al, 2022 ). In future research, we should explore the molecular mechanisms of traditional Chinese medicine in regulating ferroptosis from multiple pathways and perspectives, construct “components-targets/pathways-disease” pharmacological network, and conduct relevant clinical trials.…”