Dynamic manipulation and patterning of microparticles and cells by using TiOPc-based optoelectronic dielectrophoresis

Yang, Shih-Mo; Yu, Tung Ming; Huang, Hang Ping; Ku, Meng Yen; Hsu, Lih‐Hsing; Liu, Cheng-Hsien

doi:10.1364/ol.35.001959

Cited by 85 publications

(55 citation statements)

References 15 publications

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“…The permeabilization area can be controlled with the pulse amplitude and the degree of permeabilization can be controlled with the duration of pulses, numbers of pulses, where longer pulses provide a larger perturbation area in the cell membrane [34,35]. In earlier studies of micro/nanofluidic based single cell electroporation, authors analyze cellular content and cellular properties [36][37][38][39], transfection of cells [17,[40][41][42] and inactivating cells [43][44][45] with the use of micro-channel based electroporation [46][47][48][49], micro-capillary based electroporation [50][51][52], electroporation with solid microelectrode [36,[53][54][55], membrane sandwich based microfluidic electroporation [56,57], microarray single cell electroporation [58], optofluidic based microfluidic devices [59][60][61][62][63][64][65], etc. Table 1 describes in detail micro/nanofluidic based single cell transfection, cell lysis, cell type with species, potential difference, pulse duration, etc.…”

Section: Micro/nanofluidic Devices For Single Cell Electroporationmentioning

confidence: 99%

Recent Trends on Micro/Nanofluidic Single Cell Electroporation

Santra

Tseng

2013

Micromachines

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Abstract:The behaviors of cell to cell or cell to environment with their organelles and their intracellular physical or biochemical effects are still not fully understood. Analyzing millions of cells together cannot provide detailed information, such as cell proliferation, differentiation or different responses to external stimuli and intracellular reaction. Thus, single cell level research is becoming a pioneering research area that unveils the interaction details in high temporal and spatial resolution among cells. To analyze the cellular function, single cell electroporation can be conducted by employing a miniaturized device, whose dimension should be similar to that of a single cell. Micro/nanofluidic devices can fulfill this requirement for single cell electroporation. This device is not only useful for cell lysis, cell to cell fusion or separation, insertion of drug, DNA and antibodies inside single cell, but also it can control biochemical, electrical and mechanical parameters using electroporation technique. This device provides better performance such as high transfection efficiency, high cell viability, lower Joule heating effect, less sample contamination, lower toxicity during electroporation experiment when compared to bulk electroporation process. In addition, single organelles within a cell can be analyzed selectively by reducing the electrode size and gap at nanoscale level. This advanced technique can deliver (in/out) biomolecules precisely through a small membrane area (micro to nanoscale area) of the single cell, known as localized single cell membrane electroporation (LSCMEP). These articles emphasize the recent progress in micro/nanofluidic single cell electroporation, which is potentially beneficial for OPEN ACCESSMicromachines 2013, 4 334 high-efficient therapeutic and delivery applications or understanding cell to cell interaction.

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Section: Micro/nanofluidic Devices For Single Cell Electroporationmentioning

confidence: 99%

Recent Trends on Micro/Nanofluidic Single Cell Electroporation

Santra

Tseng

2013

Micromachines

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“…Optoelectronic tweezers (OET) have developed over the last decade as an alternative to established cell manipulation techniques. OET combines optical patterning and electric field gradients to trap cells [2][3][4][5][6]. An OET device shown in Fig.…”

Section: Introductionmentioning

confidence: 99%

Optoelectronic tweezers system for single cell manipulation and fluorescence imaging of live immune cells

Jeorrett¹,

Neale²,

Massoubre³

et al. 2014

Opt. Express

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A compact optoelectronic tweezers system for combined cell manipulation and analysis is presented. CMOS-controlled gallium nitride micro-LED arrays are used to provide simultaneous spatio-temporal control of dielectrophoresis traps within an optoelectronic tweezers device and fluorescence imaging of contrasting dye labelled cells. This capability provides direct identification, selection and controlled interaction of single T-lymphocytes and dendritic cells. The trap strength and profile for two emission wavelengths of micro-LED array have been measured and a maximum trapping force of 13.1 and 7.6 pN was achieved for projected micro-LED devices emitting at λ max 520 and 450 nm, respectively. A potential application in biological research is demonstrated through the controlled interaction of live immune cells where there is potential for this method of OET to be implemented as a compact device.

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“…To date, many literatures about the application of DEP force to separate particles using micro fluidic channels or micro-size electrodes are available (Lapizco-Encinas et al, 2004;Zhou, et al, 2006;Pysher and Hayes, 2007;Kovarik and Jacobson, 2008;Shin et al, 2008;Zhang et al, 2009;Du and Wei, 2010;Freer et al, 2010;Gagnon et al, 2010;Yang et al, 2010), and some types of electrodes to expand the DEP separation to a large scale application are also reported (Suehiro et al, 2003;Sano et al, 2011;Sano et al, 2012aSano et al, , 2012b.…”

Section: Introductionmentioning

confidence: 99%

Purification of Pt-Loaded Carbon Nanoparticles by Dielectrophoresis

Sano

Iwasaki

Tamon

2014

J. Chem. Eng. Japan

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It is generally di cult to selectively capture speci c nanoparticles from particle mixtures by conventional separation methods, for example, sedimentation and ltration. In the present study, carbon nanoparticles having an average diameter of 27 nm loaded with Pt nanoparticles having an average diameter of 1.9 nm were separated from their mixture with pristine carbon nanoparticles. For the particle-separation experiments, the Pt-loaded carbon nanoparticle (Pt-CNP) was prepared from commercial carbon black by loading Pt from a H 2 PtCl 6 aqueous solution. The electrodes used for dielectropheresis (DEP) separation were fabricated by photolithography, and the target particle mixture was dispersed in ethanol. It was found that the Pt-CNP was puri ed by the present DEP separation. The in uences of the electrode motion speed, the amplitude and the frequency of AC voltage applied on the electrodes, the duration to apply voltage in liquid, and the addition of a surfactant were investigated. It was found that there were optimized values in these operation parameters to achieve a high puri cation factor reaching approximately 92%, and the reasons of these in uences were qualitatively discussed.

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Dynamic manipulation and patterning of microparticles and cells by using TiOPc-based optoelectronic dielectrophoresis

Cited by 85 publications

References 15 publications

Recent Trends on Micro/Nanofluidic Single Cell Electroporation

Recent Trends on Micro/Nanofluidic Single Cell Electroporation

Optoelectronic tweezers system for single cell manipulation and fluorescence imaging of live immune cells

Purification of Pt-Loaded Carbon Nanoparticles by Dielectrophoresis

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