Dynamic light scattering from collagen solutions. I. Translational diffusion coefficient and aggregation effects

Abstract

SynopsisSolutions of native collagen extracted from rat tail tendons in neutral salt solution have been studied by dynamic light scattering. The spectra obtained are consistent with the presence in solution of both single rod-shaped collagen molecules and aggregates of molecules. No contribution to the spectrum has been detected a t any scattering angle from rotational diffusion of single molecules, although a measurable broadening effect is expected at high angles. * 0.2 X lo-" m2/sec a t 0.064% collagen.

Clinical applications of near-infrared diffuse correlation spectroscopy and tomography for tissue blood flow monitoring and imaging

“…DCS originates from the concept of “dynamic light scattering (DLS)”, a technology that can be used to quantify the motion of moving scatterers in a thin solution through investigating the correlation of light electric field after photons are scattered once by the moving scatterers (i.e., single light scattering) (Brown, 1993; Fletcher, 1976). The extension from single light scattering to multiple light scattering has been explored since 1980s (Maret and Wolf, 1987; Pine et al , 1988; Boas et al , 1995).…”

Stretching type II collagen with optical tweezers

“…Obrink, 1972). While there has been great progress in characterizing mechanical properties of molecules, many researchers found collagen has some flexibility (Fletcher, 1976;Thomas and Fletcher, 1979;George and Veis, 1991;Shattuck et al, 1994). Due to intrinsic limitations of various methods and assumptions in the models, however, the conflicting results were obtained using different methods to quantify the flexibility of collagen molecules (Utiyama et al, 1973;Saito et al, 1982;Nestler et al, 1983;Hofmann et al, 1984;Sun et al, 2002).…”

Preparation of Mineralized Nanofibers: Collagen Fibrils Containing Calcium Phosphate

“…This yielded a characteristic extensional flow gradient into such a pore of 1000/s. This rate can be compared to the rotational diffusivity of collagen, which is approximately 810/s 43. These two rates can be combined to provide an estimate of the Deborah number, De = 1.2.…”