Dynamic expression of miRNAs across immature and adult stages of the malaria mosquito Anopheles stephensi

Jain, Shanu; Rana, Vandita; Adak, T.; Sunil, Sujatha; Bhatnagar, Raj K.

doi:10.1186/s13071-015-0772-y

Cited by 30 publications

(42 citation statements)

References 42 publications

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“…mir-2 is widespread in invertebrates, and recent research suggests that the mir-2 family regulates insect metamorphosis by controlling the juvenile hormone signaling pathway (Lozano et al, 2015). miR-2765 was recently shown to be down-regulated in female mosquitoes compared with males (Jain et al, 2015). In S. furcifera, both mir-2 and mir-2765 were highly expressed, indicating that miRNA duplication could be conducive to the maintenance of these functional miRNAs.…”

Section: Evolutionary Analysis Of the Identified S Furcifera Mirnasmentioning

confidence: 99%

Identification and characterization of microRNAs in the white-backed planthopper,Sogatella furcifera

et al. 2016

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MicroRNAs (miRNAs) are a novel class of small, non-coding endogenous RNAs that play critical regulatory roles in many metabolic activities in eukaryotes. Reports of the identification of miRNAs in Sogatella furcifera (white-backed planthopper), the insect that acts as the only confirmed vector of the southern rice black-streaked dwarf virus (SRBSDV), are limited. In this study, a total of 382 miRNAs were identified in S. furcifera, including 106 conserved and 276 novel miRNAs, using high-throughput sequencing based on two small RNA libraries from viruliferous and non-viruliferous S. furcifera, and these miRNAs belonged to 52 conserved miRNA families and 58 S. furcifera-specific families, respectively. Comparison with miRNAs from 26 insect species and five other species in miRBase showed that more than half of the conserved miRNA families are highly conserved in Hexapoda, while other miRNAs are only conserved in nondipterans. Furthermore, 4 117 target genes predicted for the 382 identified miRNAs could be categorized into 45 functional groups annotated by Gene Ontology. Compared with non-viruliferous cells, eight up-regulated miRNAs and four down-regulated miRNAs were identified in cells inoculated with SRBSDV, among which miR-14 and miR-n98a may be involved in the immune response to SRBSDV infection. Analyses of the identified miRNAs will provide insights into the roles of these miRNAs in the regulation and expression of genes involved in the metabolism, development and viral infection of S. furcifera.

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Section: Evolutionary Analysis Of the Identified S Furcifera Mirnasmentioning

confidence: 99%

Identification and characterization of microRNAs in the white-backed planthopper,Sogatella furcifera

et al. 2016

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“…The development of DS renders the observation and identification of miRNA-mediated cleavage events more feasible. Jain et al used DS to reveal 29 cleaved targets of insect miRNAs from malaria host Anopheles stephensi [8].…”

Section: Clinical Significance Of Mirna Targets In Proteoglycans In Cmentioning

confidence: 99%

“…However, in animals (including human), destabilization or translational repression of target mRNAs are established miRNA-targeting pathways via miRNA binding to partially complementary sites [6,7]. Cleavage of miRNA targets is relatively rare, and only a few miR-NAs exhibit this "superpower" in animals [8][9][10][11][12]. HOXB8 mRNA was the first reported miRNA-directed cleavage target in mouse embryos.…”

Section: Introductionmentioning

confidence: 99%

Identification of microRNAs and their Endonucleolytic Cleavaged target mRNAs in colorectal cancer

Zhou

Tang

et al. 2020

BMC Cancer

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Background: Colorectal cancer (CRC) ranks the third among the most common malignancies globally. It is well known that microRNAs (miRNAs) play vital roles in destabilizing mRNAs and repressing their translations in this disease. However, the mechanism of miRNA-induced mRNA cleavage remains to be investigated. Method: In this study, high-throughput small RNA (sRNA) sequencing was utilized to identify and profile miRNAs from six pairs of colorectal cancer tissues (CTs) and adjacent tissues (CNs). Degradome sequencing (DS) was employed to detect the cleaved target genes. The Database for Annotation, Visualization and Integrated Discovery (DAVID) software was used for GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis. Results: In total, 1278 known miRNAs (clustered into 337 families) and 131 novel miRNAs were characterized in the CT and CN libraries, respectively. Of those, 420 known and eight novel miRNAs were defined as differentially expressed miRNAs (DEmiRNAs) by comparing the expression levels observed in the CT and CN libraries. Furthermore, through DS, 9685 and 202 potential target transcripts were characterized as target genes for 268 known and 33 novel miRNAs, respectively. It was further predicted that a total of 264 targeted genes for the 85 DEmiRNAs are involved in proteoglycans in cancer and the AMP-activated protein kinase signaling pathway. After systemic analysis of prognosisrelated miRNA targets in those cancer-related signal pathways, we found that two targets ezrin (EZR) and hematopoietic cell-specific Lyn substrate 1 (HCLS1) had the potential prognostic characteristics with CRC regarding over survival (OS) or recurrence. Conclusion: In total, we found that endonucleolytic miRNA-directed mRNA cleavage occurs in CRC. A number of potential genes targeted by CRC-related miRNAs were identified and some may have the potential as prognosis markers of CRC. The present findings may lead to an improved better appreciation of the novel interaction mode between miRNAs and target genes in CRC.

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“…To determine a potential role for miRNAs in regulating gene expression in response to kairomone exposure, we took a targeted approach, monitoring the transcript levels of four miRNAs: miR-7, miR-34, miR-317, and miR-375. These miRNAs have each been proposed to function broadly during development by targeting cell cycle progression, as well as regulating stress response, immune response, morphological development and general metabolic pathways, respectively (Li et al, 2009, Isik et al, 2016, Jain et al 2015.…”

Section: Relative Mirna Expressionmentioning

confidence: 99%

Transcriptional response to kairomone exposure in Daphnia magna: A candidate gene approach

Telli¹,

Gordon²,

Ünlü³

2020

Preprint

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Background: Daphnia (Brachiopoda, Cladocera) is a well-studied model organism providing unparalleled opportunity to test epigenetic regulation of predator avoidance mechanisms in aquatic ecosystems. The discovery of regulatory functions for microRNA molecules and recently described miRNA profiles of Daphnia make it an ideal system to probe for posttranslational regulatory mechanisms mediated by kairomone released by predatory fish. However, despite a number of studies that focused on mRNA transcript level differences, no miRNA studies associated with kairomone exposure have been reported. Results: Exposing D. magna to fish kairomone from birth to the first reproduction was found to result in the differential expression of the four miRNAs tested: miR-7, miR-34, miR-317, and miR-375. Normalized transcript levels for each miRNA were found to vary across the exposure period with no clear conserved pattern of expression despite functional target analyses by GO, COG and KEGG indicating that predicted miRNA target genes are likely involved in related biological activities. Analysis of six mRNA transcripts (Hsp70, Hsp90, actin, AKT, GYS and IGFR), identified in previous studies as kairomone-mediated genes in Daphnia magna, were also carried out. Similar to that obtained for miRNAs, the mRNA transcript levels showed varying degrees of temporal regulation across the exposure time course with the two heat shock transcripts exhibiting elevated levels at early and late time points of kairomone exposure while the AKT, GYS, and IGFR transcripts had an general decrease in expression during the first 96 hours. Conclusions: Differential mRNA expression data supports the premise of an ecological trade-off between the cost of general biological processes and that of survival under long-term kairomone stress. Transcript levels for the four miRNAs tested were found to vary across developmental time with kairomone exposure which suggests that they may have a role in regulating morphological, behavioral or physiological responses by altering target gene expression. These studies lay the foundation for future work aimed at linking miRNAs and their target transcripts to changes in the signaling events that govern Daphnia response to kairomone specific stress.

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Dynamic expression of miRNAs across immature and adult stages of the malaria mosquito Anopheles stephensi

Cited by 30 publications

References 42 publications

Identification and characterization of microRNAs in the white-backed planthopper,Sogatella furcifera

Identification and characterization of microRNAs in the white-backed planthopper,Sogatella furcifera

Identification of microRNAs and their Endonucleolytic Cleavaged target mRNAs in colorectal cancer

Transcriptional response to kairomone exposure in Daphnia magna: A candidate gene approach

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