Dynamic Changes in Serum Cytokine Profile in Rats with Severe Acute Pancreatitis

Abstract

Background and Objectives: Most published research has only investigated a single timepoint after the onset of severe acute pancreatitis (SAP), meaning that they have been unable to observe the relationship between the dynamic changes in cytokines and SAP progression. In this study, we attempted to reveal the relationship between dynamic changes in cytokine expression levels and SAP disease progression and the relationship between cytokines, using continuous large-scale cytokine detection. Materials and Methods: Seventy rats were randomly assigned to control (Con), sham operation (SO) and SAP groups. The SAP group was randomly allocated to five subgroups at 3, 6, 9, 12 and 15 h after the operation. In the SAP group, 5% sodium taurocholate was injected retrograde into the pancreatic bile duct. Animals in the SO group received a similar incision, a turning over of the pancreas. Control animals did not receive any treatment. We observed the survival, ascites fluid amount, pancreatic histopathological scores and serum amylase activity of SAP rats. We used the cytokine microarray to simultaneously detect 90 cytokines and the dynamic changes in one experiment and to analyze the correlation between cytokine expression and disease progression. Results: The mortality of SAP rats increased with an increase in time. Serum amylase activity, pancreatic histopathological scores and ascites fluid amount were time-dependent. Compared with normal rats, 69 cytokines in SAP rats were significantly changed for at least one timepoint, and 49 cytokines were significantly changed at different timepoints after SAP induction. The changes in inflammatory cytokines were significantly upregulated at 6 and 9 h and 12 h and then significantly decreased. Conclusions: The trend of cytokine expression in SAP rats was not consistent with the disease progression. The cytokine–cytokine receptor interaction and MAPK signal’s dominant cytokines were always highly expressed at various time points over the course of SAP.

Investigating potential biomarkers of acute pancreatitis in patients with a BMI>30 using Mendelian randomization and transcriptomic analysis

“…Cytokine-cytokine receptor interactions and the 12 The results of q-PCR analysis of mRNA expression levels are shown. The expression levels of HADH in patients with a high BMI were significantly greater than those in patients with AP and a high BMI (** P < 0.01) MAPK signaling pathway are associated with consistent cytokine expression throughout different stages of severe acute pancreatitis, indicating their involvement in the regulation and progression of the inflammatory response during the disease [30]. NOD-like receptors constitute a broad and intricate group of signaling regulators.…”

Research on evolution process of full‐layer incision of skin tissue under different laser incidences

“…However, in practical applications, the location of the full-layer incision in the skin tissue is relatively flexible, which cannot guarantee that the laser can be vertically incident. Therefore, it is of great significance to study the evolution rule of microstructure, collagen degeneration [20] process, and content changes of important cytokines in the process of bonding full-layer incision of skin tissue by different laser incidences in order to accelerate the prognostic process for achieving the clinical progress of laser-induced full-layer incision connection technology [21,22]. Since the thickness of skin tissue is small and the photo-thermal effect between laser and skin tissue is sensitive [23], the evolution process of microstructure corresponding to different layers of skin is an important reference to judge the healing situation of samples.…”

Research on evolution process of full-layer incision of skin tissue under different laser incidences

“…However, in practical applications, the location of the full-layer incision in the skin tissue is relatively flexible, which cannot guarantee that the laser can be vertically incident. Therefore, it is of great significance to study the evolution rule of microstructure, collagen degeneration [17] process and content changes of important cytokines in the process of bonding full-layer incision of skin tissue by different laser incidences in order to accelerate the prognostic process for achieving the clinical progress of laser induced full-layer incision connection technology [18][19] . Since the thickness of skin tissue is small and the photo-thermal effect between laser and skin tissue is sensitive [20] , the evolution process of microstructure corresponding to different layers of skin is an important reference to judge the healing situation of samples.…”