Dynamic analyte introduction and focusing in plastic microfluidic devices for proteomic analysis

Li, Yan; DeVoe, Don L.; Lee, Cheng S.

doi:10.1002/elps.200390013

Cited by 47 publications

(42 citation statements)

References 34 publications

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“…The generation of a pH gradient within a microchannel is usually accomplished by mixing carrier ampholytes (pH range 3-10 is commercially available) with the sample prior to loading [188][189][190][191][192]. The pH gradient thus establishes under high voltage between acidic and basic extreme reservoirs.…”

Section: Isoelectric Focusingmentioning

confidence: 99%

Microfluidic systems in proteomics

et al. 2003

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We present the state-of-the-art in miniaturized sample preparation, immunoassays, one-dimensional and multidimensional analyte separations, and coupling of microdevices with electrospray ionization-mass spectrometry. Hyphenation of these different techniques and their relevance to proteomics will be discussed. In particular, we will show that analytical performances of microfluidic analytical systems are already close to fulfill the requirements for proteomics, and that miniaturization results at the same time in a dramatic increase in analysis throughput. Throughout this review, some examples of analytical operations that cannot be achieved without microfluidics will be emphasized. Finally, conditions for the spreading of microanalytical systems in routine proteomic labs will be discussed.

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Section: Isoelectric Focusingmentioning

confidence: 99%

Microfluidic systems in proteomics

et al. 2003

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“…Thus, the application of a high voltage is effective to obtain high-speed separation with high efficiencies in MCIEF. 35,36 To make sure, we checked the effect of Joule heating on the efficiency. In Eq.…”

Section: Mcief On Short Channel Chipmentioning

confidence: 99%

High-speed Analysis of Proteins by Microchip Isoelectric Focusing with Linear-imaging UV Detection

2009

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High-speed analyses of proteins by microchip isoelectric focusing (MCIEF) were investigated on straight channel chips. By employing a commercially available microchip electrophoresis instrument equipped with a linear-imaging UV detector, sample proteins focused in a separation channel could be detected without a mobilization step. Typically, standard proteins were focused within 210 s at different positions in a separation channel with its total length of 38.8 mm on the basis of their pI values. The linear relationship between the focused position of each protein and its pI value was observed in the pH range from 6 to 10. The formation process of a pH gradient in the microchannel and the effects of the applied voltage and the channel length on the MCIEF separation were also investigated.

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“…Dynamic analyte introduction and focusing has been developed on plastic chip, this technique has proven to be very effective for sample loading by approximately 10-100 fold in comparison with the conventional IEF which is very important for the detection of low trace proteins. 88) 3.3.2. Thermally Generated m mCIEF This is an especial mCIEF technique where the pH gradient is not formed by means of an ampholyte, instead a tapered separation channel has been used where the pH gradient can be formed due to the difference of the temperature along the tapered channel when a current passes through, this has lead to a shallow thermal pH gradient.…”

Section: Ampholyte Dependent M Mciefmentioning

confidence: 99%

Low Viscous Separation Media for Genomics and Proteomics Analysis on Microchip Electrophoresis System

Jabasini

Murakami

Kaji

et al. 2006

Biological & Pharmaceutical Bulletin

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Microchip electrophoresis has widely grown during the past few years, and it has showed a significant result as a strong separation tool for genomic as well as proteomic researches. To enhance and expand the role of microchip electrophoresis, several studies have been proposed especially for the low viscous separation media, which is an important factor for the success of microchip with its narrow separation channels. In this paper we show an overview for the done researches in the field of low viscous media developed for the use in microchip electrophoresis. For genomic separation studies polyhydroxy additives have been used enhance the separation of DNA at low polymer concentration of HPMC (Hydroxypropylmethyl cellulose) which could keep the viscosity low. Mixtures of poly(ethylene oxide) as well as Hydroxyporpyl cellulose have been successfully introduced for chip separation. Furthermore high molecular mass polyacrylamides at low concentrations have been studied for DNA separation. A mixture of polymer nanoparticle with conventional polymers could show a better resolution for DNA at low concentration of the polymer. For the proteomic field isoelectric focusing on chip has been well overviewed since it is the most viscous separation media which is well used for the protein separation. The different types of isoelectric focusing such as the ampholyte-free type, the thermal type as well as the ampholyte-depended type have been introduced in this paper. Isoelectric focusing on chip with its combination with sodium dodecyl sulfate (SDS) page or free solution could give a better separation. Several application for this low viscous separation medias for either genomic or proteomic could clearly show the importance of this field.

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Dynamic analyte introduction and focusing in plastic microfluidic devices for proteomic analysis

Cited by 47 publications

References 34 publications

Microfluidic systems in proteomics

Microfluidic systems in proteomics

High-speed Analysis of Proteins by Microchip Isoelectric Focusing with Linear-imaging UV Detection

Low Viscous Separation Media for Genomics and Proteomics Analysis on Microchip Electrophoresis System

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