Dynamic analysis of Tapping Panel Dryness in Hevea brasiliensis reveals new insights on this physiological syndrome affecting latex production

Herlinawati, Eva; Montoro, Pascal; Ismawanto, Sigit; Syafaah, Afdholiatus; Aji, Martini; Giner, Michel; Flori, Albert; Gohet, Eric; Oktavia, Fetrina

doi:10.1016/j.heliyon.2022.e10920

Cited by 5 publications

(2 citation statements)

References 35 publications

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“…Herlinawati et al. ( 2022 ) advocated that the Mg 2+ measurements in TPD latex have relatively poor reproducibility and are susceptible to seasonal effects. Therefore, these results may not be a true reflection of the Mg 2+ content in the C‐serum.…”

Section: Resultsmentioning

confidence: 99%

“…This may be due to the instability of the lutoids in TPD latex, which are prone to rupture and release of Mg 2+ , resulting in inaccurate C-serum Mg 2+ content determination results. Herlinawati et al (2022) advocated that the Mg 2+ measurements in TPD latex have relatively poor reproducibility and are susceptible to seasonal effects. Therefore, these results may not be a true reflection of the Mg 2+ content in the C-serum.…”

Section: Rubber Biosynthesis Activity In Latex From Healthy and Tpd-a...mentioning

confidence: 99%

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Downregulation of HbFPS1 affects rubber biosynthesis of Hevea brasiliensis suffering from tapping panel dryness

et al. 2023

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SUMMARY Tapping panel dryness (TPD) is a century‐old problem that has plagued the natural rubber production of Hevea brasiliensis. TPD may result from self‐protective mechanisms of H. brasiliensis in response to stresses such as excessive hormone stimulation and mechanical wounding (bark tapping). It has been hypothesized that TPD impairs rubber biosynthesis; however, the underlying mechanisms remain poorly understood. In the present study, we firstly verified that TPD‐affected rubber trees exhibited lower rubber biosynthesis activity and greater rubber molecular weight compared to healthy rubber trees. We then demonstrated that HbFPS1, a key gene of rubber biosynthesis, and its expression products were downregulated in the latex of TPD‐affected rubber trees, as revealed by transcriptome sequencing and iTRAQ‐based proteome analysis. We further discovered that the farnesyl diphosphate synthase HbFPS1 could be recruited to small rubber particles by HbSRPP1 through protein–protein interactions to catalyze farnesyl diphosphate (FPP) synthesis and facilitate rubber biosynthesis initiation. FPP content in the latex of TPD‐affected rubber trees was significantly decreased with the downregulation of HbFPS1, ultimately resulting in abnormal development of rubber particles, decreased rubber biosynthesis activity, and increased rubber molecular weight. Upstream regulator assays indicated that a novel regulator, MYB2‐like, may be an important regulator of downregulation of HbFPS1 in the latex of TPD‐affected rubber trees. Our findings not only provide new directions for studying the molecular events involved in rubber biosynthesis and TPD syndrome and contribute to rubber management strategies, but also broaden our knowledge of plant isoprenoid metabolism and its regulatory networks.

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Section: Resultsmentioning

confidence: 99%

Section: Rubber Biosynthesis Activity In Latex From Healthy and Tpd-a...mentioning

confidence: 99%

Downregulation of HbFPS1 affects rubber biosynthesis of Hevea brasiliensis suffering from tapping panel dryness

et al. 2023

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Bark transcriptome analyses reveals molecular mechanisms involved in tapping panel dryness occurrence and development in rubber tree (Hevea brasiliensis)

Yue,

Wang,

Xia

et al. 2024

Gene

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Construction and analysis of the tapping panel dryness-related lncRNA/circRNA-miRNA-mRNA ceRNA network in latex of Hevea brasiliensis

Liu,

Yuan,

et al. 2023

Plant Physiology and Biochemistry

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Dynamic analysis of Tapping Panel Dryness in Hevea brasiliensis reveals new insights on this physiological syndrome affecting latex production

Cited by 5 publications

References 35 publications

Downregulation of HbFPS1 affects rubber biosynthesis of Hevea brasiliensis suffering from tapping panel dryness

Downregulation of HbFPS1 affects rubber biosynthesis of Hevea brasiliensis suffering from tapping panel dryness

Bark transcriptome analyses reveals molecular mechanisms involved in tapping panel dryness occurrence and development in rubber tree (Hevea brasiliensis)

Construction and analysis of the tapping panel dryness-related lncRNA/circRNA-miRNA-mRNA ceRNA network in latex of Hevea brasiliensis

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