Dye‐membrane Interaction and Its Changes During Nerve Excitation

Tasaki, Ichiji; Warashina, Akira

doi:10.1111/j.1751-1097.1976.tb06810.x

Cited by 44 publications

(47 citation statements)

References 23 publications

(6 reference statements)

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“…The environment of the dye molecules has to be assumed to differ with the layer of attachment. One advantage of this alternative is the consistency with what has been proposed for the TNS fluorescence signal when the dye is injected intracellularly and excited with polarized light [66,67]. 7.…”

Section: B) Absorption Signals Using Polarized Lightmentioning

confidence: 91%

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Mechanical, thermal, and optical changes of the nerve membrane associated with excitation.

Watanabe

1986

Jpn.J.Physiol

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In this minireview, some of the recent experimental findings on the excitable membranes of nerve fibers obtained by nonelectrophysiological means are discussed. This minireview, however, does not treat the vast literature on the "optical probes of the membrane potential," which is adequately covered by other excellent reviews [14,22,23,30,52].When the nerve fiber is excited, it transiently changes many of its physical properties. From the biological point of view, the most significant is the change in membrane potential, since the transmission of information along the nerve fiber is mediated by the electric local current. Other physical changes are significant mainly because they contain information on the molecular mechanism of the excitation process. They are usually very small for an easy detection, but progress in the techniques of extracting small signals from noise allowed us to discover and examine some of these signals. In this minireview, several recent findings on mechanical, thermal, and optical signals will be discussed.

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Section: B) Absorption Signals Using Polarized Lightmentioning

confidence: 91%

“…TAsAxi and WARASHINA [65,66] found that the sign of the absorption signal is reversed when the direction of E-vector of the incident light was changed by 90° under some experimental conditions. For example, a squid giant axon was stained externally with crystal violet, and illuminated with 540 nm light.…”

Section: B) Absorption Signals Using Polarized Lightmentioning

confidence: 99%

Mechanical, thermal, and optical changes of the nerve membrane associated with excitation.

Watanabe

1986

Jpn.J.Physiol

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“…In other words, the direction of absorbance responses found when NK2935 was added intracellularly was opposite to that found when NK2935 was added extracellularly. This mirror image relation is called "sidedness" (COHEN, 1973) and is also known in other potential probe dyes (COHEN et al, 1971;TASAKI and WARASHINA, 1976). The sidedness is widely accepted as a sign that the dyes cannot penetrate a given membrane (COHEN, 1973).…”

Section: External and Internal Optical Spikesmentioning

confidence: 96%

New optical spike in frog twitch muscle fibers injected with a potential probe dye NK2935.

Sato

Fujino

1986

Jpn.J.Physiol

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Absorbance responses posterior to action potentials were recorded from frog twitch fibers loaded by an impermeant potential probe dye NK2935 that was chemically identical with WW781. Extracellularly loaded NK2935-related responses (EOS) increased in absorbance around 640 nm wavelengths and were like action potential in waveform but were slightly preceded by action potential. Model analysis showed that EOS consisted of two components, namely 39% non-delay component reflected with sarcolemma action potential and 61 % first-order delay component reflected with transverse tubule (T) action potential (delay-constant i1 = 0.48 ms). The T-disrupted fibers showed only the non-delayed response. Intracellularly injected NK2935-related responses (IOS) were also composed of a single spike of the same direction swing as in the EOS case described by the term "ipsidirection," but were clearly preceded by EOS and followed by Ca transient, birefringence, transparency and latency relaxation responses. The T-disrupted fibers showed a small "sidedness" response characterized by both opposite direction swing, namely "contradirection" and non-delay against EOS. Model analysis showed that IOS consisted of two main components, namely second-order delay component with ipsidirection (x2 =0.8 ms) and the further high-order delay component with contradirection. Based on spatio-temporal heterogeneity of NK2935 binding inside fibers and potential-absorbance relation in sarcolemma, the former origin was thought to be in junctional sarcoplasmic reticulum (jSR) responding to T excitation with a transient "positive polarization" estimated roughly 20 mV. Similarly, the latter was possibly in free SR where a slow "negative polarization" estimated -0.9 mV might appear associated with Ca release.

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“…These substances are endowed with the property of fluorescence, the spectrum of which This work has been supported by the F6d6ration de Cardiologie and the Association Claude Bernard. varies with differences of the transmembrane potential [9,10,11].…”

Section: Introductionmentioning

confidence: 99%

A study of electrical activation of the heart by laser spectrometry

Fillette

Nassif

1987

Int J Cardiac Imag

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The action potentials of working myocardium and conduction tissues, traditionally recorded by intracellular glass microelectrodes, may also be studied at a distance without physical contact using an optical system. The tissues have to be stained with a dye which fluoresces when illuminated by a laser beam, and the spectrum of the fluorescence must be sensitive to variations of the transmembrane action potential. The responses obtained by the microelectrode and the optical systems were tested on several different preparations (sheep and mouse myocardium). Three types of signals were obtained: asynchronous, synchronous and mixed, related to myocardial contraction, the action potential and the excitation-contraction couple, respectively. The use of continuous and pulsed mode laser measurements by optoelectronic methods (photomultiplier, monochromator), and imaging of the electrical activation by a CCD video camera may lead to the development of high definition mapping of myocardial activation which would be used for studying arrhythmias in experimental and even clinical models.

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Dye‐membrane Interaction and Its Changes During Nerve Excitation

Cited by 44 publications

References 23 publications

Mechanical, thermal, and optical changes of the nerve membrane associated with excitation.

Mechanical, thermal, and optical changes of the nerve membrane associated with excitation.

New optical spike in frog twitch muscle fibers injected with a potential probe dye NK2935.

A study of electrical activation of the heart by laser spectrometry

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