A study of electrical activation of the heart by laser spectrometry

Fillette, F; Nassif, George

doi:10.1007/bf01784304

Cited by 4 publications

(3 citation statements)

References 11 publications

(18 reference statements)

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“…Single-channel optical recording systems with a laser light source have been used in the early studies of the optical recording of electrical activities of cardiac cells [59][60][61][62].…”

Section: Laser Scanning Systemmentioning

confidence: 99%

Optical Mapping Approaches to Cardiac Electrophysiological Functions.

Sakai

Kamino²

2001

JJP

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Electrophysiology has been one of the most important strategies in cardiac physiology since the mid19th century [1]. The methodology in this field has been improved with the development of electronics, and quite sophisticated techniques using extracellular electrodes, intracellular microelectrodes, and patch electrodes are now widely used. Recently, optical methods for monitoring membrane potential with fast voltage-sensitive dyes were introduced as a new powerful tool for studying cardiac electrical functions. In this article, optical studies of the electrophysiological function of the vertebrate heart are reviewed. HISTORICAL BACKGROUND OF OPTICAL METHODS FOR MONITORING ELECTRICAL ACTIVITYIn 1968, changes in intrinsic light scattering, in birefringence, and in extrinsic 8-anilino-naphtalene-1-sulfonate (ANS) fluorescence associated with an action potential in the squid giant axon were first reported by Cohen et al. [2] and by Tasaki et al. [3]. At first these experiments were carried out to learn about conformation and/or structural changes that might occur during nerve excitation: it was hoped that the observed optical changes might be related to some molecular mechanism(s) of electrical excitation [4]. In additional experiments, however, Cohen, Salzberg, and co-workers indicated that fluorescent changes depended on changes in transmembrane potential [5], and they soon suggested that optical methods might be used for monitoring membrane potential in cells or cellular processes not accessible to microelectrodes [6,7]. Key words: voltage-sensitive dye, optical recording, electrical activity, heart. Abstract:Recently, optical methods for monitoring membrane potential with fast voltage-sensitive dyes have been introduced as a powerful tool for studying cardiac electrical functions. These methods offer two principal advantages over more conventional electrophysiological techniques. One is that optical recordings may be made from very small cells that are inaccessible to microelectrode impalement, and the other is that multiple sites/regions of a preparation can be monitored simultaneously to provide spatially resolved mapping of electrical activity. The former has made it possible to record spontaneous electrical activities in early embryonic precontractile hearts, and the latter has been applied for mapping of the propagation patterns of electrical activities in the cardiac tissue. In this article, optical studies of the electrophysiological function of the vertebrate heart are reviewed.

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“…Single-channel optical recording systems with a laser light source have been used in the early studies of the optical recording of electrical activities of cardiac cells [59][60][61][62].…”

Section: Laser Scanning Systemmentioning

confidence: 99%

Optical Mapping Approaches to Cardiac Electrophysiological Functions.

Sakai

Kamino²

2001

JJP

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“…This offers the advantage of increased spatial resolution, compared with recordings from electrode arrays (Fluhler et al, 1985). Recent advances in cardiac imaging applications include: the development of probes with enhanced signal-to-noise characteristics or red-shifted dyes (Matiukas et al, 2007;Lee et al, 2019;Martišienė et al, 2020) to increase imaging depth, incorporation of mechanical uncouplers or motion tracking to reduce/remove motion artifact (Fedorov et al, 2007;Bourgeois et al, 2011;Jaimes et al, 2016b;Zhang et al, 2016;Kappadan et al, 2020), development of panoramic imaging techniques (Bray et al, 2000;Kay et al, 2004;Lee et al, 2017;Gloschat et al, 2018), and improvements in photodetector and light source technology (Grinvald et al, 1981;Fillette and Nassif, 1987;Salama et al, 1987).…”

Section: Introductionmentioning

confidence: 99%

KairoSight: Open-Source Software for the Analysis of Cardiac Optical Data Collected From Multiple Species

et al. 2021

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Cardiac optical mapping, also known as optocardiography, employs parameter-sensitive fluorescence dye(s) to image cardiac tissue and resolve the electrical and calcium oscillations that underly cardiac function. This technique is increasingly being used in conjunction with, or even as a replacement for, traditional electrocardiography. Over the last several decades, optical mapping has matured into a “gold standard” for cardiac research applications, yet the analysis of optical signals can be challenging. Despite the refinement of software tools and algorithms, significant programming expertise is often required to analyze large optical data sets, and data analysis can be laborious and time-consuming. To address this challenge, we developed an accessible, open-source software script that is untethered from any subscription-based programming language. The described software, written in python, is aptly named “KairoSight” in reference to the Greek word for “opportune time” (Kairos) and the ability to “see” voltage and calcium signals acquired from cardiac tissue. To demonstrate analysis features and highlight species differences, we employed experimental datasets collected from mammalian hearts (Langendorff-perfused rat, guinea pig, and swine) dyed with RH237 (transmembrane voltage) and Rhod-2, AM (intracellular calcium), as well as human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) dyed with FluoVolt (membrane potential), and Fluo-4, AM (calcium indicator). We also demonstrate cardiac responsiveness to ryanodine (ryanodine receptor modulator) and isoproterenol (beta-adrenergic agonist) and highlight regional differences after an ablation injury. KairoSight can be employed by both basic and clinical scientists to analyze complex cardiac optical mapping datasets without requiring dedicated computer science expertise or proprietary software.

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Section: Introductionmentioning

confidence: 99%

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A study of electrical activation of the heart by laser spectrometry

Cited by 4 publications

References 11 publications

Optical Mapping Approaches to Cardiac Electrophysiological Functions.

Optical Mapping Approaches to Cardiac Electrophysiological Functions.

KairoSight: Open-Source Software for the Analysis of Cardiac Optical Data Collected From Multiple Species

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