Dye Decolourisation Using Two Klebsiella Strains

Abstract

This study aimed to decolourise different dyes using two Klebsiella strains (Bz4 and Rz7) in different concentrations and incubation conditions. Azo (Evans blue (EB)) and triphenylmethane (brilliant green (BG)) dyes were used individually and in mixture. The toxicity of the biotransformation products was estimated. Both strains had a significant potential to decolourise the dyes in the fluorone, azo and triphenylmethane classes. The type and concentration of dye affects the decolourisation effectiveness. Differences in the dye removal potential were observed particularly in the main experiment. The best results were obtained for Bz4 in the samples with EB (up to 95.4 %) and dye mixture (up to 99 %) and for Rz7 with BG (100 %). The living and dead biomass of the strain Bz4 highly absorbs the dyes. Significant effect of the process conditions was noticed for both strains. The best results were obtained in static and semistatic samples (89–99 %) for the removal of EB and a mixture of dyes and in static samples (100 %) for BG. The decrease in zootoxicity (from class IV/V) was noticed in all samples with living biomass of the strain Bz4 (to class III/IV) and in samples with single dyes for Rz7 (to class III/IV). The decrease in phytotoxicity (from class III/IV) was noticed for Bz4 in the samples with BG and a mixture (to class III) and for Rz7 in the samples with BG (to class III). The process conditions did not affect the changes in toxicity after the process.

Fed-Batch Decolourization of Mixture of Brilliant Green and Evans Blue by Bacteria Species Applied as Pure and Mixed Cultures: Influence of Growth Conditions

“…This stage requires more time than physical sorption on the surface of bacterial cells and depends on various factors such as temperature, pH, redox potential, dye structure and concentration, and the properties of the bacteria. (Jang et al 2 0 0 5 ; S r i n i v a s a n a n d Vi r a r a g h a v a n 2 0 1 0 ; Vijayaraghavan and Yun 2008;Wu et al 2009a;Zabłocka-Godlewska et al 2015).…”

“…This dye mixture has not been examined up to now, with the exception of other studies by the authors (e.g. Przystaś et al 2013;Zabłocka-Godlewska et al 2014;Zabłocka-Godlewska et al 2015). The choice for study the Evans blue and brilliant green was firstly dictated by the fact that they are representatives of two most popular classes of applied dyes, secondly due to the common use exactly these dyes we can expect their presence in the wastewaters and then in aquatic ecosystems.…”

Isolation of a Microorganic Strain for the High Volume Degradation of Aniline Blue and Its Application in Natural Sewage Treatment

“…More than 0.7 million tons of triphenylmethane dyes are used annually in textile production and other related industries [1] [2]. However, synthetic dyes are no-DOI: 10.4236/cmb.2018.84008 150 Computational Molecular Bioscience toriously difficult to degrade [3], so approximately 15% of synthetic dyes end up being released into the environment [4] [5] [6], with potentially harmful health and ecological outcomes [7]. In aqueous ecosystems, synthetic dyes can inhibit photosynthesis and in terrestrial ecosystems [8] [9], the primary broken-down molecules of synthetic dyes are known to have a detrimental effect on microorganisms [10], animals and grain crops [11] [12].…”

“…Biological treatments are considered more environmentally friendly, less costly and more likely to succeed than physicochemical methods [4] [8] [12]. Microbial degradation of triphenylmethane dyes, in particular, has received a lot of attention [7] [19] [20].…”

“…Confronted with this problem, bacterium would seem to offer the optimum alternative [12] [16] [31]. To date, Pseudomonas, Bacillus, Sphingomonas, Aeromonas, Citrobacter, Escherichia, Desulphovibrio, Proteus, Schewanella and Alcaligenes have had success in removing triphenylmethane dyes [4] [6].…”