Isolation of a Microorganic Strain for the High Volume Degradation of Aniline Blue and Its Application in Natural Sewage Treatment

Abstract

Aniline blue, one of the triphenylmethane dyes, is the most commonly produced and used of these dyes yet it is also the most dangerous and the most serious cause of pollution amongst them. An exploration of aniline blue degradation is likely to facilitate an understanding of the degradation mechanism for a range of related dyes. In this study, we managed to isolate a particular strain of microorganism, identified to be Lysinibacillus fusiformis N019a, which showed a significant capacity for aniline blue degradation in both laboratory tests and natural sewage treatment. In analysis aided by a UV-Visible spectrophotometer, we found that 96.7% of aniline blue had degraded within 24 hours under laboratory conditions. When treating natural sewage, 80.1% of the aniline blue was removed after just 16 hours. Further analysis has shown that Lysinibacillus fusiformis N019a has a strong resistance to Cu 2+ , Mn 2+ , Zn 2+ , and Pb 2+. We also found that the degradation product of aniline blue by Lysinibacillus fusiformis N019a was of reduced toxicity to plants and microbes.

New cost-effective mediator enhanced enzymatic degradation of aniline blue

“…Aniline blue, an extensively used toxic dye, is a member of triphenylmethane dyes (Li et al, 2020). The trisulfonic acid group in aniline blue confers high polarity to the molecule, thereby deterring its degradation and increasing environmental toxicity (Li et al, 2018). Aniline blue is degraded by chemical (Zhang et al, 2017), physical (Ayed et al, 2010), or biological methods (Anastasi et al, 2011).…”

“…The chemical and physical methods have disadvantages of high cost, low efficiency, and secondary contamination (Ayed et al, 2017;Holkar et al, 2014). Microbial degradation is reportedly unsuitable for aquatic environments, and the dye degradation potential of microorganisms in aquatic environments is low, probably because these microorganisms do not grow efficiently in aquatic environments (Li et al, 2018).…”