Dwarfism in Brassica napus L. induced by the over-expression of a gibberellin 2-oxidase gene from Arabidopsis thaliana

Zhou, Bo; Lin, Juan; Peng, Wusheng; Peng, Dan; Zhuo, Ya; Zhu, Dengfeng; Huang, Xingqun; Tang, Dongying; Guo, Ming; He, Reqing; Zhang, Jihong; Li, Xiushan; Zhao, Xiaoying; Liu, Xuanming

doi:10.1007/s11032-010-9530-1

Cited by 41 publications

(20 citation statements)

References 54 publications

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“…In addition, Leaves of the transgenic plants were darker green due to increased chlorophyll contents (Table 1). Similar characteristics have already been observed in transgenic plants of various species overexpressing the GA2ox gene (Dijkstra et al 2008;Gargul et al 2013;Schomburg et al 2003;Zhou et al 2012). Type I and Type II have already been obtained in our previous study (Otani et al 2013).…”

supporting

confidence: 84%

“…On the other hand, overexpression of the GA2ox gene induced dwarf phenotypes in transgenic Arabidopsis thaliana, Nicotiana tabacum (Schomburg et al 2003), Torenia fournieri (Niki et al 2006), Solanum spp. (Dijkstra et al 2008), Brassica napus (Zhou et al 2012), Kalanchoe blossfeldiana and Petunia hybrida (Gargul et al 2013). Thus genetic transformation with the GA2ox gene may be an alternative approach to transformation with wildtype A. rhizogenes or the rol genes for producing dwarf ornamental plants.…”

mentioning

confidence: 99%

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Horticultural characterization of a tetraploid transgenic plant of <i>Tricyrtis</i> sp. carrying the gibberellin 2-oxidase gene

Ôtani

Ishibe

Inthima

et al. 2014

Plant Biotechnology

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The gibberellin 2-oxidase catalyzes the bioactive gibberellins or their immediate precursors to inactive forms. We have previously produced transgenic plants of the liliaceous plant Tricyrtis sp. containing the GA2ox gene from the linderniaceous plant Torenia fournieri. These transgenic plants showed dwarf phenotypes as expected but unfortunately had no flowers or only small, unopened flowers. Recently, one newly produced transgenic line (G2-55) formed fully opened flowers. G2-55 showed a moderately dwarf phenotype and the shoot length decreased to 63.4% of that of the control, non-transgenic plants. No significant differences in the number of flowers per shoot and in the flower size were observed between G2-55 and the control. Flow cytometry analysis and chromosome observation showed that G2-55 was tetraploid (2n=4x=52), whereas the other transgenic lines producing no or only small flowers were diploid (2n=2x=26) as the mother plant. Pollen fertility of G2-55 was 81.2% as determined by acetocarmine staining. The tetraploidy in G2-55 might be resulted from somaclonal variation of embryogenic calluses used as a target material for Agrobacterium-mediated transformation. The tetraploid transgenic plant G2-55 may be useable not only directly as a potted plant, but also as a material for further breeding of Tricyrtis spp.

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supporting

confidence: 84%

mentioning

confidence: 99%

Horticultural characterization of a tetraploid transgenic plant of <i>Tricyrtis</i> sp. carrying the gibberellin 2-oxidase gene

Ôtani

Ishibe

Inthima

et al. 2014

Plant Biotechnology

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“…In addition, GA 3 stimulated the total sugar and glucosinolates accumulation in the seeds. Previous studies by our research group indicated that the heterologous expression of AtGA2ox8, which is a GA inactivation gene, in oilseed rape enhanced photosynthetic capacity (Zhou et al, 2011), siliques, seed yield, and seed oil content, but decreased glucosinolate content (Zhao et al, 2010;Zhou et al, 2012). These findings in combination with the results of the current study indicate that both endogenous and exogenous GAs regulate seed development, seed storage accumulation, and glucosinolate synthesis.…”

Section: Discussionsupporting

confidence: 86%

Effect of exogenous gibberellin on reserve accumulation during the seed filling stage of oilseed rape

Huang¹,

He²,

Liao³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Exogenous gibberellins (GAs) are widely applied to increase crop yields, with knowledge about the physiological functioning and biochemistry mechanisms of these phytohormones improving; however, information remains limited about the effect of GAs on seed filling. In this study, the siliques (containing the seeds) of oilseed rape (Brassica napus L.) were treated with GA 3 at 3 stages of seed filling. We confirmed that GA 3 regulates the deposition of storage reserves in developing seeds. The percentage of crude fat in the seeds increased during the early stage, but remained stable during the middle and late stages. In comparison, the percentage of total protein decreased during the early and middle stages, but significantly increased during the late stage. In addition, Q-PCR was employed to analyze the expression level of related genes in 2828 X.Q. Huang et al.©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (2): 2827-2839 (2014) response to GA 3 . It was found that the expression of WRI and ABI3 transcription factors corresponded to crude fat content and total protein content, respectively. The expression of storage reserve related genes DGAT, MCAT, SUC2, and GPT was consistent with crude fat content, whereas the expression of Napin corresponded to total protein content. The results of this study indicate that exogenous GA 3 has a different effect on storage reserve deposition in seed during different stages of seed filling, and the effect might be achieved via changing the expression of related genes.

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“…Ten plants from plot were randomly selected for agronomic traits analysis. The plant height (PH), internode length (IL), internode number (IN), rst branch height (FBH), main in orescence length (MIL), number of effective primary branches (NPB), number of siliques on raceme (NSR), siliques per plant (SPP), length of siliques (LS), seeds per silique (SPS), thousand-seed weight (TSW) and yield per plant (YPP), were measured and counted as previously described [39,40]. Signi cant differences were determined by Student's t-test using SPSS version 25 (SPSS Inc, Chicago).…”

Section: Agronomic Traits Analysismentioning

confidence: 99%

Characterization and Fine Mapping of a New Dwarf Mutant in Brassica Napus

Xiang

Zhang

et al. 2020

Preprint

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Background: Plant height is an important plant architecture character closely related to yield performance of many crops. Reasonable reduction of plant height of crops is beneficial for enhancing lodging resistance and improving yield. Results: In the present study, we described a Brassica napus dwarf mutant bnd2 induced by ethyl methanesulfonate (EMS) mutagenesis. Compared to wild type, bnd2 showed shorter stature, shorter hypocotyl, as well as shorter petiole leaves. We crossed the bnd2 mutant with its wild type and found that the ratio of the mutant to the wild type in the F2 population was close to 1:3, indicating that bnd2 is a recessive mutation of a single locus. Following bulked segregant analysis (BSA) by resequencing, BND2 was located into the 13.77 Mb-18.08 Mb interval of chromosome A08, with a length of 4.31 Mb. After fine mapping with SNP and InDel markers, the gene was narrowed to a 140-Kb interval ranging from 15.62 Mb to 15.76 Mb. According to reference genome annotation, there are 27 genes in the interval, and one of them BnaA08g20960D has a SNP type variation in the intron between the mutant and its parent, which may be the candidate gene conferring to BND2. The hybrid line derived from a cross between the mutant bnd2 and a commercial cultivar L329 has similar plant height but higher grain yield than the commercial cultivar, suggesting that the allele bnd2 is benefit for hybrid breeding of lodging resistance and high yield in rapeseed.Conclusion: In this study, we found a fresh resource and a new locus for dwarf in rapeseed, which may be benefit for functional analysis of genetic mechanism of plant architecture and grain yield in rapeseed.

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Dwarfism in Brassica napus L. induced by the over-expression of a gibberellin 2-oxidase gene from Arabidopsis thaliana

Cited by 41 publications

References 54 publications

Horticultural characterization of a tetraploid transgenic plant of <i>Tricyrtis</i> sp. carrying the gibberellin 2-oxidase gene

Horticultural characterization of a tetraploid transgenic plant of <i>Tricyrtis</i> sp. carrying the gibberellin 2-oxidase gene

Effect of exogenous gibberellin on reserve accumulation during the seed filling stage of oilseed rape

Characterization and Fine Mapping of a New Dwarf Mutant in Brassica Napus

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