Characterization and Fine Mapping of a New Dwarf Mutant in Brassica Napus

Abstract

Background: Plant height is an important plant architecture character closely related to yield performance of many crops. Reasonable reduction of plant height of crops is beneficial for enhancing lodging resistance and improving yield. Results: In the present study, we described a Brassica napus dwarf mutant bnd2 induced by ethyl methanesulfonate (EMS) mutagenesis. Compared to wild type, bnd2 showed shorter stature, shorter hypocotyl, as well as shorter petiole leaves. We crossed the bnd2 mutant with its wild type and found that the ratio of the mutant to the wild type in the F2 population was close to 1:3, indicating that bnd2 is a recessive mutation of a single locus. Following bulked segregant analysis (BSA) by resequencing, BND2 was located into the 13.77 Mb-18.08 Mb interval of chromosome A08, with a length of 4.31 Mb. After fine mapping with SNP and InDel markers, the gene was narrowed to a 140-Kb interval ranging from 15.62 Mb to 15.76 Mb. According to reference genome annotation, there are 27 genes in the interval, and one of them BnaA08g20960D has a SNP type variation in the intron between the mutant and its parent, which may be the candidate gene conferring to BND2. The hybrid line derived from a cross between the mutant bnd2 and a commercial cultivar L329 has similar plant height but higher grain yield than the commercial cultivar, suggesting that the allele bnd2 is benefit for hybrid breeding of lodging resistance and high yield in rapeseed.Conclusion: In this study, we found a fresh resource and a new locus for dwarf in rapeseed, which may be benefit for functional analysis of genetic mechanism of plant architecture and grain yield in rapeseed.

Inheritance of dwarfism and narrow lobed-leaf in two rapeseed (Brassica napus L.) mutant lines

“…However, in previous studies on other mutants, monogenic inheritance was reported, with a major dominant locus in mutant NJ7982 [ 25 ] or a major gene with additive effects in mutant Bzh [ 23 ] and in mutant NDF-1 [ 24 ]. In addition [ 41 ], described a dwarf mutant bnd2 in rapeseed using EMS mutagenesis and inheritance analysis on F 2 population revealed that the dwarf phenotype of bnd2 was controlled by a single recessive gene. Following bulked segregant analysis (BSA) by resequencing [ 41 ], reported that BND2 to be located in the 13.77–18.08 Mb interval of chromosome A08, with a length of 4.31 Mb [ 41 ].…”

“…In addition [ 41 ], described a dwarf mutant bnd2 in rapeseed using EMS mutagenesis and inheritance analysis on F 2 population revealed that the dwarf phenotype of bnd2 was controlled by a single recessive gene. Following bulked segregant analysis (BSA) by resequencing [ 41 ], reported that BND2 to be located in the 13.77–18.08 Mb interval of chromosome A08, with a length of 4.31 Mb [ 41 ]. found that the gene was narrowed to a 140-Kb interval ranging from 15.62 Mb to 15.76 Mb, after using a fine mapping with single nucleotide polymorphism (SNP) and insertion/deletion (InDel) markers.…”

“…found that the gene was narrowed to a 140-Kb interval ranging from 15.62 Mb to 15.76 Mb, after using a fine mapping with single nucleotide polymorphism (SNP) and insertion/deletion (InDel) markers. According to reference genome annotation, there were 27 genes in the interval, of which BnaA08g20960D had an SNP type variation in the intron between the mutant and its parent, which may be the candidate gene corresponding to BND2 [ 41 ].…”

BnaC01.BIN2, a GSK3-like kinase, modulates plant height and yield by regulating cell elongation in Brassica napus

“…''green revolution''(Chandler Jr 1969;Xuchu 1987) has increased the interest in mapping dwarf allele in several crops, including rapeseed(Li et al 2019;Li et al 2021;Zhao et al 2017;Zhao et al 2019). Although several genes have been cloned to control plant height in rapeseed, these genes have seldom been applied to rapeseed breeding because they sigi cantly trade-offs in yield related traits(Li et al.…”