Untitled

Phosphorylation of cell cycle and apoptosis regulatory protein-1 by stress activated protein kinase P38γ is a novel mechanism of apoptosis signaling by genotoxic chemotherapy

“…Protein Assay Kit was purchased from Bio-Rad Laboratories (Hercules, CA) while Chemi-luminescence Reagent was purchased from Amersham Biosciences (Piscataway, NJ). Structure and synthesis of experimental compounds CARP-1 Functional Mimetic (CFM)-4, -5, and -16 have been described previously ( 14 ). Clinical grade Adriamycin, Cisplatin, 5-fluouracil, Gemcitabine, and Paclitaxel were obtained from the Karmanos Cancer Institute Pharmacy, Wayne State University, Detroit, MI.…”

“…All the cell culture media were also supplemented with 10%FBS, 100units/ml of penicillin, and 100μg/ml of streptomycin, and the cells were maintained at 37°C and 5% CO 2 . Generation, characterization, and culture of drug (ADR or Cisplatin)-resistant human TNBC MDA-MB-468 and MDA-MB-231 cells, ADR-resistant murine 4T1 cells, as well as MDA-MB-468 cells expressing reduced CARP-1 have been detailed before ( 9 , 14 ). For cell growth and MTT studies, the cells were cultured in fresh media with 5%-10% FBS prior to their treatments with various agents.…”

“…The stable sublines are listed in Supplementary Table S4 , and were generated by transfecting various plasmids into the MDA-MB-468 and Hela cells followed by selection in the presence of 800μg/ml neomycin using described methods ( 8 , 9 , 14 ). The cell lysates from wild-type, untransfected cells, neomycin-resistant pools, or individual sublines were then subjected to IP and WB analyses as below.…”

“…At the end of treatment, cells were incubated with MTT reagent (0.5mg/ml) for 2-4 hours at 37°C, followed by addition of DMSO to solubilize formazan. The plate was read at 570nm in a plate reader, and histograms indicating levels of cell viability were generated by plotting the net absorbances as described ( 8 , 9 , 14 ). For western blot and immunoprecipitation experiments, logarithmically growing cells were either untreated or treated with different agents for various time periods followed by lysis of cells to prepare protein extracts.…”

<p>Enhancing Drug Delivery for Overcoming Angiogenesis and Improving the Phototherapy Efficacy of Glioblastoma by ICG-Loaded Glycolipid-Like Micelles</p>

“…ICG-loaded cRGD-modified glycolipid-like nanoparticles (cRGD-CSOSA/ICG) were prepared by dialysis as in our previous study. 40 Briefly, hydrophobic ICG (5 mg/mL in DMSO) was added into a CSOSA or cRGD-CSOSA solution (2 mg/mL in deionized water).Then the mixture was stirred at room temperature for 2 h in the dark. After that, the solution was dialyzed against DI water for 24 h and then centrifuged at 8000 rpm for 10 min to remove the unloaded ICG.…”