Durum wheat × maize crosses for haploid wheat production: Influence of parental genotypes and various experimental factors

Cherkaoui, S.; Lamsaouri, O.; Chlyah, Averil; Chlyah, Hassan

doi:10.1046/j.1439-0523.2000.00433.x

Cited by 32 publications

(26 citation statements)

References 25 publications

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“…The most common of the various media are MS medium as originally presented (Murashige and Skoog, 1962) or variants of it (MS/2), and B5 medium. Cherkaoui et al (2000) obtained the highest green plant production from embryos cultured on MS/2 and B5 media. Adding indoleacetic acid and kinetin can increase the rate of green plant production (Zhang et al, 1996;Cherkaoui et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

“…Cherkaoui et al (2000) obtained the highest green plant production from embryos cultured on MS/2 and B5 media. Adding indoleacetic acid and kinetin can increase the rate of green plant production (Zhang et al, 1996;Cherkaoui et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

“…Most papers state that small embryos (≤1.5 mm) must be rejected (Almouslem et al, 1998) and that embryo rescue should be attempted 10-12 days (Inagaki and Tahir, 1990) or 18-21 days (Savaskan, et al, 1997) after pollination of wheat plants with maize pollen (DAP). Cherkaoui et al (2000) achieved the highest haploid embryo development percentage when embryo rescue was performed at 14 DAP; when embryo rescue was attempted earlier the embryos were smaller and less differentiated, producing fewer green plants.…”

Section: Introductionmentioning

confidence: 99%

“…The most common synthetic growth regulator used is 2,4-D (dichlorophenoxy acetic acid). It has been applied in several ways: sprinkling of the spike, as a drop, injection into the upper internode, and spike immersion (Laurie and Bennett, 1988;Cherkaoui et al, 2000). Other successfully applied regulators include dicamba (Savaskan et al, 1997;Campbell et al, 1998), gibberellic acid (Almouslem et al, 1998) and silver nitrate (O'Donoughue and Bennett, 1994;Almouslem et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

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Factors Affecting Doubled Haploid Plant Production Via Maize Technique in Bread Wheat

Xynias¹,

Koufalis²,

Gouli-Vavdinoudi³

et al. 2015

Acta Biologica Cracoviensia S. Botanica

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The effect of two in planta factors (growth conditions, genotype) and two in vitro factors (time of embryo rescue, embryo rescue medium) on doubled haploid (DH) plant production in bread wheat via maize technique was investigated in nine F 1 hybrids produced after crossing four bread wheat cultivars. During the first year one group of F 1 plants was grown in a field and at the proper stage pollinated with maize pollen (sweet corn population). In parallel, a second group of F 1 plants was grown in a growth chamber and pollinated as in the former group. In the second growing season the experiment was repeated but only field-grown plants were used. All the produced haploid embryos were cultured in three different media and the resulting 146 haploid plants were subsequently treated with aqueous solution of colchicine. Finally, 86 doubled haploid plants were obtained. We noted that the growing conditions of the parental plants and the intervening time between day of pollination and day of embryo rescue influenced the percentage of haploid embryo production. Culture medium also influenced haploid and doubled haploid plant production. The two media (MS/2, B5) were found equally effective. Most of the haploid embryos originated from the Penios × Acheloos cross, whereas most of the doubled haploid plants were produced from the KVZ × Penios cross. Doubled haploid plants were produced from all crosses.K Ke ey y w wo or rd ds s: : Culture, doubled haploid, embryos, haploid, maize technique, Triticum aestivum L.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Factors Affecting Doubled Haploid Plant Production Via Maize Technique in Bread Wheat

Xynias¹,

Koufalis²,

Gouli-Vavdinoudi³

et al. 2015

Acta Biologica Cracoviensia S. Botanica

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“…To speed up the durum wheat improvement programmes, a durum wheat × maize system has so far been exploited for haploid induction (Almouslem et al 1998;Cherkaoui et al 2000). However, the haploid embryo formation frequency of the system has been the major problem.…”

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confidence: 99%

Dihaploid induction in tetraploid durum wheat (Triticum durum L.) using pollen of Imperata cylindrica

Chaudhary¹,

Mahato²,

Kaila³

et al. 2015

CZECH J GENET PLANT

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Chaudhary H.K., Mahato A., Kaila V., Rather S.A., Tayeng T. (2015): Dihaploid induction in tetraploid durum wheat (Triticum durum L.) using pollen of Imperata cylindrica. Czech J. Genet. Plant Breed., 51: 142-147. Doubled haploidy breeding protocols have revolutionized the varietal development programmes in bread wheat, however, the protocols have not proved much efficient in durum wheat. Presently, the durum wheat × maize system is being widely followed for haploid induction but the frequency of haploid development is very poor which has limited its application in durum wheat improvement programmes. In order to formulate an efficient wide hybridization-mediated approach for haploid induction in durum wheat, different genotypes of durum wheat were subjected to hybridization with Imperata cylindrica, a wild perennial grass for the first time in this laboratory. The investigation was carried out for two seasons. During the 1 st year, the investigation was carried out on one genotype only, Langdon, in order to notice the development of haploid embryos and the factors that influence the haploid induction frequency. The most important factor influencing the embryo formation frequency was found to be the concentration of 2,4-dichlorophenoxyacetic acid (2,4-D). Upon pollinating the emasculated spikes of durum wheat genotypes, various concentrations of 2,4-D were injected into the uppermost internode of wheat culm for three consecutive days after pollination to find out the most responding concentration for haploid induction. During the next year, the protocol was applied to eight durum wheat genotypes. The frequency of haploid induction parameters varied with the durum wheat genotypes as well as 2,4-D concentration used. The mean pseudoseed and embryo formation frequency over all the genotypes ranged from 30.2 to 56.3% and 1.2 to 18.4%, respectively. The average embryo formation frequency over all the genotypes was found to be highest (18.4%) at 250 mg/l 2,4-D whereas it was superior for the genotypes WH 896 and Langdon over all the 2,4-D concentrations. At the most responding 2,4-D concentration (250 mg/l), the genotype A-9-30-1 exhibited the highest embryo formation frequency (32.1%). The ploidy status of the developed embryos was identified using cytological analysis carried out on the rootlets of the tissue culture generated plantlets.

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Progress in Doubled Haploid Technology in Higher Plants

Wędzony

Forster²,

Żur

et al.

Advances in Haploid Production in Higher Plants

120

113

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Durum wheat × maize crosses for haploid wheat production: Influence of parental genotypes and various experimental factors

Cited by 32 publications

References 25 publications

Factors Affecting Doubled Haploid Plant Production Via Maize Technique in Bread Wheat

Factors Affecting Doubled Haploid Plant Production Via Maize Technique in Bread Wheat

Dihaploid induction in tetraploid durum wheat (Triticum durum L.) using pollen of Imperata cylindrica

Progress in Doubled Haploid Technology in Higher Plants

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