Progress in Doubled Haploid Technology in Higher Plants

Wędzony, Maria; Forster, B. P.; Żur, Iwona; Golemiec, Elżbieta; Szechyńska‐Hebda, Magdalena; Dubas, Ewa; Gotębiowska, G.

doi:10.1007/978-1-4020-8854-4_1

Cited by 121 publications

(127 citation statements)

References 237 publications

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“…Our results on the effect of genotype are in line with previous reports (Sadasivaiah, 1999;Cherkaoui et al, 2000;Wedzony et al 2009) that the maize technique is less dependent on genotype: doubled haploid lines were produced from all the wheat F 1 hybrids, although some responded better than others. The observed differences in the androgenetic response of the germplasm we used could be attributed to differences in the genotypes' response to climatic conditions, as suggested by Matzk and Mann (1994) and O'Donoughue and Bennett (1994), or to the different genetic backgrounds of the evaluated F 1 wheat hybrids.…”

Section: Discussionsupporting

confidence: 92%

Factors Affecting Doubled Haploid Plant Production Via Maize Technique in Bread Wheat

Xynias¹,

Koufalis²,

Gouli-Vavdinoudi³

et al. 2015

Acta Biologica Cracoviensia S. Botanica

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The effect of two in planta factors (growth conditions, genotype) and two in vitro factors (time of embryo rescue, embryo rescue medium) on doubled haploid (DH) plant production in bread wheat via maize technique was investigated in nine F 1 hybrids produced after crossing four bread wheat cultivars. During the first year one group of F 1 plants was grown in a field and at the proper stage pollinated with maize pollen (sweet corn population). In parallel, a second group of F 1 plants was grown in a growth chamber and pollinated as in the former group. In the second growing season the experiment was repeated but only field-grown plants were used. All the produced haploid embryos were cultured in three different media and the resulting 146 haploid plants were subsequently treated with aqueous solution of colchicine. Finally, 86 doubled haploid plants were obtained. We noted that the growing conditions of the parental plants and the intervening time between day of pollination and day of embryo rescue influenced the percentage of haploid embryo production. Culture medium also influenced haploid and doubled haploid plant production. The two media (MS/2, B5) were found equally effective. Most of the haploid embryos originated from the Penios × Acheloos cross, whereas most of the doubled haploid plants were produced from the KVZ × Penios cross. Doubled haploid plants were produced from all crosses.K Ke ey y w wo or rd ds s: : Culture, doubled haploid, embryos, haploid, maize technique, Triticum aestivum L.

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Section: Discussionsupporting

confidence: 92%

Factors Affecting Doubled Haploid Plant Production Via Maize Technique in Bread Wheat

Xynias¹,

Koufalis²,

Gouli-Vavdinoudi³

et al. 2015

Acta Biologica Cracoviensia S. Botanica

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“…Breeders can make new cultivars with features from DH lines and be confident about completely homozygous plants. Moreover, DH lines can be used in the production of genetic maps, which help in the search for quantitative traits loci (QTL), conjugated with molecular markers and connected with many physiological processes, such as receptivity to the effectiveness of androgenesis or tolerance to biotic and abiotic stresses [1,2]. Haploids can be obtained using a number of methods, namely: from male gametophyte by androgenesis, using anther cultures or isolated microspores cultures [3][4][5][6][7][8], or from female gametophytes by gynogenesis or wide crossing, usually with maize (a method also known as chromosomes elimination) [1,9,10].…”

Section: Introductionmentioning

confidence: 99%

Production of double haploids in oat (Avena sativa L.) by pollination with maize (Zea mays L.)

et al. 2013

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Production of double haploids in oat (Avena sativa L.) by pollination with maize (Zea mays L.) Abbreviations 2,4-D -2,4-dichlorophenosyacetic acid; dicamba -3,6-dichloro-2-methoxybenzoic acid; DH -double haploid. IntroductionModern crop breeding has a number of advantages when using DH plants, such as shortening of the production time of new varieties and the possibility of using them in research. Classical methods of production of new varieties, based on a selection in every generation, take six to ten years. Biotechnological methods allow for a shortening of this time, even to one vegetative season. Breeders can make new cultivars with features from DH lines and be confident about completely homozygous plants. Moreover, DH lines can be used in the production of genetic maps, which help in the search for quantitative traits loci (QTL), conjugated with molecular markers and connected with many physiological processes, such as receptivity to the effectiveness of androgenesis or tolerance to biotic and abiotic stresses [1,2]. Haploids can be obtained using a number of methods, namely: from male gametophyte by androgenesis, using anther cultures or isolated microspores cultures [3][4][5][6][7][8], or from female gametophytes by gynogenesis or wide crossing, usually with maize (a method also known as chromosomes elimination) [1,9,10]. In comparison to anther culture, pollination with maize has an advantage because all regenerated haploid embryos are green, whereas in anther culture many of them are albino. The method of chromosome elimination that has been used in these experiments includes the following stages: after emasculation of cereal flowers the stigma is pollinated by maize, the pollen then germinates, a pollen tube is formed [11,12] and grows into the cereal embryo sac, where the cereal egg is fertilised by the maize sperm nuclei. A hybrid zygote is produced. The hybrid zygotes Keywords: Wide crossing • 2,4-D • Dicamba • Oat haploid embryosAbstract: The aim of the study was to optimize the method of oat haploid production by pollination with maize. Seventeen oat genotypes were used in the experiment. Various factors influencing the growth and development of ovaries and embryo production were investigated: genotype, time of pollination, growth regulators and time of their application. Emasculated before anthesis, oat florets were pollinated with maize pollen after 0, 1 or 2 days. Next, one of two auxins analogues (2,4-D or dicamba) were applied to oat pistils. These auxins had no significant influence on the number of enlarged ovaries and embryos. The time of application of these growth regulators had a significant influence on embryo production. Haploid embryos were obtained from all used genotypes, although the frequency of enlarged ovaries and obtained embryos did not differ markedly between the genotypes. On average, 85% of ovaries were enlarged and 11.7% of them produced haploid embryos. Depending on the regeneration medium, 24-41% of embryos were germinated, of which 12% had developed into green plant...

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“…For that reason the development of new tools is needed to accelerate obtaining homozygosity in breeding of pea and lupins. Homozygosity can be attained by selfing successive generations or by hapoidization of hybrids using different in vitro techniques and doubling chromosomes in haploid plants (for review see, e.g., [1][2][3]). Pisum and Lupinus species are known to be recalcitrant to in vitro culture [4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Preliminary results of in vitro culture of pea and lupin embryos for the reduction of generation cycles in single seed descent technique

et al. 2013

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Pea and lupin plants have been potentially rich sources of protein for human and animal diets. Currently, in Poland the main source of protein is soya cake, imported to Poland in amount of 2 mln tons per year. For substitution of soya with domestic protein plants breeding methods for higher and stable yield of grain legumes should be developed. About 12 generations are needed to develop a new cultivar and under central or north European climate conditions only one generation per year is feasible in the field. For that reason the development of new tools is needed to accelerate obtaining homozygosity in breeding of pea and lupins. Homozygosity can be attained by selfing successive generations or by hapoidization of hybrids using different in vitro techniques and doubling chromosomes in haploid plants (for review see, e.g., [1][2][3]). Pisum and Lupinus species are known to be recalcitrant to in vitro culture [4][5][6][7]. In spite of numerous studies focused on the production of doubled haploids (DH), no important results have been noted to date [8][9][10][11]. For shortening the breeding cycle, the single seed descent technique (SSD) in combination with in vitro culture of immature embryos may be an alternative to the DH system. Currently, SSD populations are frequently used as alternatives to DH populations in genetic and genomic studies (see, for example, [12,13]). The SSD technique connected with embryo in vitro culture allows the attainment of homozygous lines in a relatively short time. Such an approach applied to the development of winter barley SSD lines enabled the shortening of the generation cycle to ca. 4 months (including verbalization) [14]. Ochatt et al. [15] proposed the reduction of pea generation cycles based on in vitro culture of embryos and a greenhouse strategy, but no information was found on embryo culture for accelerating breeding process in lupins.The aim of the present studies was to establish in vitro conditions for the culture of pea, and narrow-leafed and yellow lupin embryos as the first step in research aimed at shortening generation cycles in the SSD technique used to accelerate the development of homozygous populations. Material and methodsMaterial for the studies consisted of two pea cultivars (Pisum sativum L.) -Cysterski and Akord, two narrow-leafed lupin cultivars (Lupinus angustifolius L.) -Kadryl and Sonet, and two yellow lupin cultivars (L. luteus L.) -Mister and Taper. Pea and lupin cultivar seeds were sown in the experimental field at Wiatrowo near Poznań. Pods from each pea and lupin cultivar were detached 21-28 days after flowering, when seeds were still-green but fully developed. Seeds taken out of pods were sterilized by successive dips into ethanol 70% (3 min) and AbstractThe aim of the studies was to establish in vitro conditions for the culture of pea and lupin embryos as the first step in the development of an in vitro assisted single seed descent technique for the attainment of homozygous populations. Materials for the study included of pea, and narrow-leafed a...

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Progress in Doubled Haploid Technology in Higher Plants

Cited by 121 publications

References 237 publications

Factors Affecting Doubled Haploid Plant Production Via Maize Technique in Bread Wheat

Factors Affecting Doubled Haploid Plant Production Via Maize Technique in Bread Wheat

Production of double haploids in oat (Avena sativa L.) by pollination with maize (Zea mays L.)

Preliminary results of in vitro culture of pea and lupin embryos for the reduction of generation cycles in single seed descent technique

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