Durable spatiotemporal surveillance of Caenorhabditis elegans response to environmental cues

Kopito, Ronen B.; Levine, Erel

doi:10.1039/c3lc51061a

Cited by 56 publications

(77 citation statements)

References 33 publications

(50 reference statements)

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“…28 Various microfluidic devices have been reported that trap multiple animals for high-throughput imaging, 29 automated sorting, 30 quantification of muscular forces, 31 chemosensory analyses, 25,32 electrotaxis, 33,34 thermosensory analyses, 35,36 and whole-animal stress responses. 24,37 A long term culture platform for C. elegans based on microfluidic approaches has also been presented. 24,27 Here, we introduce a device that facilitates streamlined and high-resolution imaging of the mechanosensory response in combination with mechanical stimulation in C. elegans .…”

Section: Introductionmentioning

confidence: 99%

“…24,37 A long term culture platform for C. elegans based on microfluidic approaches has also been presented. 24,27 Here, we introduce a device that facilitates streamlined and high-resolution imaging of the mechanosensory response in combination with mechanical stimulation in C. elegans . Importantly, physical access to the immobilized worm is necessary for applying mechanical stimuli.…”

Section: Introductionmentioning

confidence: 99%

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Pneumatic stimulation of C. elegans mechanoreceptor neurons in a microfluidic trap

et al. 2017

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New tools for applying force to animals, tissues, and cells are critically needed in order to advance the field of mechanobiology, as few existing tools enable simultaneous imaging of tissue and cell deformation as well as cellular activity in live animals. Here, we introduce a novel microfluidic device that enables high-resolution optical imaging of cellular deformations and activity while applying precise mechanical stimuli to the surface of the worm’s cuticle with a pneumatic pressure reservoir. To evaluate device performance, we compared analytical and numerical simulations conducted during the design process to empirical measurements made with fabricated devices. Leveraging the well-characterized touch receptor neurons (TRNs) with an optogenetic calcium indicator as a model mechanoreceptor neuron, we established that individual neurons can be stimulated and that the device can effectively deliver steps as well as more complex stimulus patterns. This microfluidic device is therefore a valuable platform for investigating the mechanobiology of living animals and their mechanosensitive neurons.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Pneumatic stimulation of C. elegans mechanoreceptor neurons in a microfluidic trap

et al. 2017

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“…DAF-16, one of the members of the FOXO transcription factor that acts in dauer formation, longevity, fat metabolism, stress response, and innate immunity [42,43]. In live C. elegans under standard culture condition, the DAF-16 protein is broadly expressed both in the cytoplasm and nuclei.…”

Section: Stress Response To Different Concentration Of Bacteriamentioning

confidence: 99%

Logarithmic bacterial gradient chip for analyzing the effects of dietary restriction on C. elegans growth

Wang

et al. 2018

Sensors and Actuators B: Chemical

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a b s t r a c t C. elegans is a commonly used model organism for studying the basic mechanisms of lifespan extension by dietary restriction (DR). Progress has been limited, however, by the lack of an automated system for quantitative analysis of this effect of bacterial diet. In this work, multifunctional logarithmic gradientcustomizing microfluidic device, which takes advantage of hydrodynamics, was developed to long-term maintain worm culture on the chip with parallel live imaging and generate logarithmic concentration gradient of bacteria. We successfully employed this platform to investigate the longevity and stress response of the worms in response to various concentrations of bacterial food. The results indicated that the lifespan extension of the worms was induced by DR when bacteria density was 10 8 cells mLand further dilutions would reduce the lifespan, which proposed a new dietary restriction regime in C. elegans. In addition, imaging analysis showed that DR regulated the subcellular localization DAF-16 in a way different from starvation that reduced lifespan of the worms. The platform allows not only horizontal studies of C. elegans in response to various environments over a wide range of concentrations, but also multiparameter evaluation of longevity in the worms with high temporal resolution, providing clues to better understand the aging process and help age-related drug screen.

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“…Although challenging in traditional culture systems, controllable spatiotemporal exposure to stimuli for long periods is possible with microfluidic devices. For instance, chips have been developed for life-long culture and imaging of C. elegans under a variety of conditions such as exposure to chemicals, immobilization for high-resolution imaging, and behavior tracking [51,52,[86][87][88][89]. These experiments, however, impose several difficulties.…”

Section: Control Of Flowmentioning

confidence: 99%

Microfluidic Platforms for Quantitative Biology Studies in Model Organisms

Porto

Rouse

San-Miguel

et al. 2016

Microfluidic Methods for Molecular Biology

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The use of lab-on-chip tools has been adopted in a wide variety of scientific fields. Hundreds of applications that speed up, miniaturize, or enable otherwise unfeasible assays have emerged in the last couple of decades [1,2]. The microfluidic toolbox offers several advantages which make it a very attractive resource for biological studies: reduced sample volume, control of spatiotemporal chemical compositions, streamlined assays, precise and predictable fluid flow regimes, portability, and integration with sensors, actuators, controllers, and automation systems [1][2][3]. The main drive of the field has thus far focused on the development of microfluidic tools that replace conventional methods with proof-ofprinciple applications. However, widespread adoption of these technologies for fundamental research is still in progress. Microfluidics has nonetheless had a significant impact in fundamental biological studies with model organisms [4,5]. In this article, we provide an overview of the current state of the field, the impacts of microfluidics in model organism research, and the outlook, challenges, and opportunities for the future.

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Durable spatiotemporal surveillance of Caenorhabditis elegans response to environmental cues

Cited by 56 publications

References 33 publications

Pneumatic stimulation of C. elegans mechanoreceptor neurons in a microfluidic trap

Pneumatic stimulation of C. elegans mechanoreceptor neurons in a microfluidic trap

Logarithmic bacterial gradient chip for analyzing the effects of dietary restriction on C. elegans growth

Microfluidic Platforms for Quantitative Biology Studies in Model Organisms

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