Duplex PCR for differentiation of the vaccine strain Brucella suis S2 and B. suis biovar 1 from other strains of Brucella spp.

Nan, Wenlong; Tan, Pengfei; Wang, Yong; Xu, Zouliang; Mao, Kairong; Peng, Daxin

doi:10.1016/j.tvjl.2014.05.033

Cited by 10 publications

(13 citation statements)

References 8 publications

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“…Being a rapid and simple assay, Duplex-PCR was used for differentiation of Rev-1 vaccine strain from B. melitensis field strain (Alvarez et al, 2017), B. suis S2 vaccine strain from field strains of Brucella spp (Nan et al,2014) .…”

Section: Resultsmentioning

confidence: 99%

“…Simple duplex-PCR can be more useful for direct detection of Brucella organism in clinical specimen including milk and differentiating it with vaccine strain S19. A relatively simple duplex-PCR has been used for differentiation of Rev-1 vaccine strain from B.melitensis field strain (Alvarez et al, 2017), B. suis S2 vaccine strain from field strains of Brucella spp (Nan et al, 2014). Multiplex PCR has been developed for differentiation of B.abortus RB51 and B.abortus S19 from other filed isolate of Brucella (Sharifi Yazdi et al,2008).…”

Section: Introductionmentioning

confidence: 99%

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Single-tube duplex-PCR for specific detection and differentiation of Brucella abortus S19 vaccine strain from other Brucella spp

Das

Kumar

Chakravarti

et al. 2018

IJAR

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A simple single-tube duplex-PCR assay was optimized for rapid and sensitive detection and differentiation of Brucella abortus (B.abortus) S19 vaccine strain from other Brucella spp.(B.abortus 544,B abortus S99,B.melitensis 16M,B.suis).This assay was optimized using two primer pairs that were designed, one targeting genus specific multicopy IS711 and another eryC for the development of the duplex-PCR assay. Specificity of the assay was assessed using DNA templates from various Brucella species (B.abortus, B.melitensis, B.suis) and non Brucella bacteria like Staphylococcus aureus, Proteus vulgaris, Pasteurella multocida, Pseudomonas aeruginosa, Citrobacter freundii, E. coli, Salmonella enteritidis, Campylobacter jejuni and Listeria monocytogenes. The assay was also evaluated on spiked milk samples with known B.abortus cultures. The assay was able to detect up to 2.47 x 103CFU of B. abortus S99 organism in spiked milk sample. The assay was further validated in 53 clinical specimens (aborted foetal stomach content). Results confirm that Single tube duplex-PCR assay was useful for early and rapid detection and differentiation of B. abortus vaccine strain S19 from other Brucella spp in milk as well as in clinical specimen.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Single-tube duplex-PCR for specific detection and differentiation of Brucella abortus S19 vaccine strain from other Brucella spp

Das

Kumar

Chakravarti

et al. 2018

IJAR

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“…The Bruce-ladder multiplex PCR primers were used to identify the species of Brucella genus [7] . The duplex PCR primers were used to differentiate B. suis S2 from B. suis 1330 [8] . All samples were examined by PCR in a total volume of 30 μL, with 13 μL ddH 2 O, 15 μL master mix, 0.5 μL of each primer and 1.5 μL DNA template.…”

Section: Pcr Amplificationmentioning

confidence: 99%

Kuzeybatı Çin’de Atık Koyun Fötuslarından Brucella suis S2 İzolasyonu

Zhang¹,

Wang²,

Yi³

et al. 2019

Kafkas Univ Vet Fak Derg

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This study aimed to investigate the cause of abortion in a traditional sheep farm. Here, 31 specimens were examined by PCR targeting the Brucella outer membrane protein gene 22 (omp22), and 25 (80.6%, 25/31) were found to be positive. Totally, 3 Brucella suis S2 and 10 Brucella melitensis were isolated from 31 aborted fetuses in which one B. suis S2 and B. melitensis were isolated from a same aborted fetus. All of these isolates were identified by PCR and conventional bacterial tests. These 3 B. suis isolates and the reference strain B. suis S2 were all identified as B. suis biovar 1 and the 10 B. melitensis isolates were all identified as B. melitensis biovar 3. This study suggests that B. suis S2 can partially induce abortion in pregnant ewes. Hence, for the sake of safety, it is need to develop a new Brucella vaccine to protect pregnant sheep from brucellosis.

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“…including B. suis . 22 , 82 – 84 These assays were further refined using data from available B. suis genomes for the development of real-time assays that allow rapid and inexpensive identification of Brucella with high sensitivity. 22 , 85 – 88 These assays have been complemented with multiple-locus variable-number tandem-repeat PCR-based methods that allow strain comparisons in addition to species/biovar identification.…”

Section: Diagnosis Of Swine Brucellosismentioning

confidence: 99%

Swine brucellosis: current perspectives

Olsen¹,

Tatum²

2016

VMRR

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Brucella suis is a significant zoonotic species that is present in domestic livestock and wildlife in many countries worldwide. Transmission from animal reservoirs is the source of human infection as human-to-human transmission is very rare. Although swine brucellosis causes economic losses in domestic livestock, preventing human infection is the primary reason for its emphasis in disease control programs. Although disease prevalence varies worldwide, in areas outside of Europe, swine brucellosis is predominantly caused by B. suis biovars 1 and 3. In Europe, swine are predominantly infected with biovar 2 which is much less pathogenic in humans. In many areas worldwide, feral or wild populations of swine are important reservoir hosts. Like other Brucella spp. in their natural host, B. suis has developed mechanisms to survive in an intracellular environment and evade immune detection. Limitations in sensitivity and specificity of current diagnostics require use at a herd level, rather for individual animals. There is currently no commercial vaccine approved for preventing brucellosis in swine. Although not feasible in all situations, whole-herd depopulation is the most effective regulatory mechanism to control swine brucellosis.

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Duplex PCR for differentiation of the vaccine strain Brucella suis S2 and B. suis biovar 1 from other strains of Brucella spp.

Cited by 10 publications

References 8 publications

Single-tube duplex-PCR for specific detection and differentiation of Brucella abortus S19 vaccine strain from other Brucella spp

Single-tube duplex-PCR for specific detection and differentiation of Brucella abortus S19 vaccine strain from other Brucella spp

Kuzeybatı Çin’de Atık Koyun Fötuslarından Brucella suis S2 İzolasyonu

Swine brucellosis: current perspectives

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