Duplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal samples

Marinheiro, Juliana Cristina; Sanalios, Roberta Braga; Santos, Daniela Carvalho dos; Costa, Cristóvão Alves da; Hársi, Charlotte Marianna

doi:10.1590/s0074-02762009000100018

Cited by 4 publications

(8 citation statements)

References 23 publications

(23 reference statements)

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“…Acute respiratory tract infections (ARTI) are regarded as important causes of morbidity and mortality in pediatric patients. This refers to both outpatient visits and 20-40% of hospitalized patients (1,2). The significant impact of these infections on developing countries is based on the fact that nearly 50% of pediatric consultations are related to the respiratory tract infection (RTI) (3).…”

Section: Introductionmentioning

confidence: 99%

“…The significant impact of these infections on developing countries is based on the fact that nearly 50% of pediatric consultations are related to the respiratory tract infection (RTI) (3). The world health organization (WHO) ranks the lower RTIs as the leading cause of disease worldwide, which accounts for 4 million deaths per year (2,4). RTI can be life-threatening depending on the causative agent and host condition.…”

Section: Introductionmentioning

confidence: 99%

“…A high incidence of RTI in children is due to increasing exposure of young children to siblings and friends with RTI, childcare centers, and environmental factors. Additionally, susceptibility to ARTI may be due to inherited disorders of the immune system, which are responsible for 45-60% of all acute respiratory diseases in infants and young children (2,3). Most of these infections are caused by respiratory syncytial virus (RSV), influenza virus A or B (FluV), parainfluenza virus (PIV), rhinovirus (RV), and human adenovirus (HAdV).…”

Section: Introductionmentioning

confidence: 99%

“…Human adenoviruses (HAdV) are grouped into seven species of A to G and to date, 56 different types (HAdV-1 to HAdV-56) have been described based on serology, whole genome sequencing, or phylogenomics (7)(8)(9). Adenovirus accounts for 5-15% of upper and lower RTIs in infants and children hospitalized for respiratory disease (2,10). Clinical signs of an AdV infection are rather nonspecific and variable and include tonsillopharyngitis, conjunctivitis, pneumonia, gastroenteritis, hepatitis, and hemorrhagic cystitis (2).…”

Section: Introductionmentioning

confidence: 99%

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Detection of Adenovirus Infection Among Children With Acute Respiratory Disease During 2010-2012 in Shiraz, Iran

Moattari

Pirbonyeh

Yaghoobi

2014

Arch Pediatr Infect Dis

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Background: Acute viral respiratory infection (ARVI) is a common cause of morbidity and mortality especially in pediatric patients. Among important respiratory pathogens, adenovirus is a most prevalent cause of upper and lower respiratory tract infection. Objectives: The aim of the present study was to determine the prevalence of adenovirus infection in pediatric patients with respiratory symptoms. Patients and Methods: This cross-sectional study was carried out from November 2010 to February 2012. An in-house nested-PCR was used to detect adenovirus in nasopharyngeal swabs from 328 children, aged 19 years or younger, with acute upper respiratory tract symptoms referred to the hospitals affiliated with Shiraz University of Medical Sciences. Results: Adenovirus was detected in 22% of 328 nasopharyngeal swabs and the highest rate of hospitalization (74%) due to ARVI associated with fever, cough and muscular ache was found in children older than five years. Conclusions: The results of this study showed that acute respiratory infection due to adenovirus in Shiraz mostly occur during November to February. In contrast to other studies, adenovirus infection was more prevalent in children aged older than 5 years.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Detection of Adenovirus Infection Among Children With Acute Respiratory Disease During 2010-2012 in Shiraz, Iran

Moattari

Pirbonyeh

Yaghoobi

2014

Arch Pediatr Infect Dis

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“…Human adenoviruses are important causes of respiratory tract infections and are responsible for one third of deaths in children and adults in worldwide [11].…”

Section: Discussionmentioning

confidence: 99%

Adenoviruses Type D in Patients with Respiratory Tract Infection Symptoms

Javid¹,

Moradi²,

Mohebbi³

et al. 2017

OALib

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Different Human Adenoviruses (HAdV) serotypes are associated with epidemic respiratory infections. The aim of this study was to detect HAdV from patients with respiratory symptoms and typing virus circulating in North of IRAN. Four hundred nasopharyngeal aspirate samples were obtained from patients with respiratory symptoms. Clinical data were collected. After DNA extraction, Polymerase Chain Reaction (PCR) was done for conserved Hexon region. Positive PCR products were used for sequencing grouping. 37 cases (9.2%) were positives. Clinical symptoms as cough (73%), body (67/6%), and fever (64/9%) were found in 35 infected patients (94/5%) and two patients (15/5%) had no symptoms. All samples were belonged to HAdV of species D (HAdV-8). Prevalence of respiratory adenovirus infection in our area is consistent with other studies in Iran and ocular type of HAdV8 was the frequent adenovirus serotype circulating in acute respiratory disease in Golestan province.

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