Duodenal jejunal bypass attenuates non-alcoholic fatty liver disease in western diet-obese rats

Abstract

PURPOSE:To evaluate the effects of duodenal-jejunal bypass (DJB) on serum and hepatic profiles of obese rats fed on a western diet (WD). METHODS:Twenty eight male Wistar rats were fed a standard rodent chow diet (CTL group) or WD ad libitum. After 10 weeks, WD rats were submitted to sham (WD SHAM) or duodenal-jejunal bypass (WD DJB). Body weight, fat pad depots, glycemia, insulinemia, HOMA-IR, TyG, lipids profile and hepatic analyses were evaluated two months after surgery. RESULTS:The WD SHAM group presented greater obesity, hyperglycemia, hyperinsulinemia, insulin resistance, hypertriglyceridemia and hepatic steatosis than the CTL group. WD DJB rats presented decreased serum glucose and insulin resistance, when compared to WD SHAM animals, without changes in insulinemia. In addition, DJB surgery normalized serum TG and attenuated TG accumulation and steatosis in the liver of the WD DJB group. Hepatic ACC and FAS protein expressions were similar in all groups.CONCLUSION: Duodenal-jejunal bypass attenuates hepatic parameters of non-alcoholic fatty liver disease in obese rats fed on a western diet.

Duodenal-jejunal bypass normalizes pancreatic islet proliferation rate and function but not hepatic steatosis in hypothalamic obese rats

“…The consumption of a high-fat or western diet leads to the development of hepatic steatosis (14,25). Conversely, it has been suggested that approximately 60% of liver fat is derived from circulating NEFA in individuals who eat a normal fat-containing diet (24).…”

“…Duodenal-jejunal bypass (DJB) surgery seems to be a good strategy to improve glucose homeostasis and NAFLD in obese rodents, induced by hyper-caloric diet or genetic alterations (11–14). However, information about the effects of DJB upon hypothalamic obesity is scarce, possibly due to a lack of experimental models that mimic the features of this syndrome.…”

Effects of duodenal-jejunal bypass on structure of diaphragm in western diet obese rats

“…In order to investigate anypossible beneficial effects of DJB surgery, the animals submitted to the cafeteria diet continued to receive the same diet after the surgery. DJB surgery is a bariatric procedure capable of improving glucose homeostasis in animal models of acquired diabetes and dietinduced obesity 13,20 , regardless of body weight loss. In this study, there were no changes in body weight, the weight of retroperitoneal and periepididymal fator the Lee index eight weeks after the DJB.…”

“…Studies have shown that in animal models of acquired diabetes or obesity, prior to producing weight loss, this procedure improves glucose homeostasis [10][11][12] , the lipid profile 11 , attenuates fatty liver disease 13 and prevents atherosclerosis 14 . However, there are no reports on the effects of this type of surgery on the morphology of the diaphragm in obese mice.…”

Role of bariatric surgery in treatment of non-alcoholic fatty liver disease

“…1 The first sign of NAFLD is accumulation of hepatic fat (steatosis) that progress to non-alcoholic steatohepatitis (NASH), liver inflammation, fibrosis, cirrhosis and hepatocellular carcinoma. 4 Current NAFLD and NASH treatments include weight loss obtained by life style modification and pharmaceuticals but have poor effects in patients with body mass index >35 kg/m 2 . 5 Some evidences showed bariatric surgery (BS) could alter the course of disease.…”