DUF538 Protein Super Family is Predicted to be the Potential Homologue of Bactericidal/Permeability-Increasing Protein in Plant System

Gholizadeh, Ashraf; Kohnehrouz, Samira Baghban

doi:10.1007/s10930-013-9473-6

Cited by 17 publications

(20 citation statements)

References 29 publications

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“…Two DUF538 proteins were also found only in grain and not in vegetative organs of B. distachyon (Bradi1g25270 and Bradi4g21520). This protein family was recently nominated as the potential structural and functional homolog of the BPI (Bactericidal/Permeability Increasing) protein in plants .…”

Section: Discussionmentioning

confidence: 99%

Cell wall proteomic of Brachypodium distachyon grains: A focus on cell wall remodeling proteins

et al. 2015

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Cell walls play key roles during plant development. Following their deposition into the cell wall, polysaccharides are continually remodeled according to the growth stage and stress environment to accommodate cell growth and differentiation. To date, little is known concerning the enzymes involved in cell wall remodeling, especially in gramineous and particularly in the grain during development. Here, we investigated the cell wall proteome of the grain of Brachypodium distachyon. This plant is a suitable model for temperate cereal crops. Among the 601 proteins identified, 299 were predicted to be secreted. These proteins were distributed into eight functional classes; the class of proteins that act on carbohydrates was the most highly represented. Among these proteins, numerous glycoside hydrolases were found. Expansins and peroxidases, which are assumed to be involved in cell wall polysaccharide remodeling, were also identified. Approximately half of the proteins identified in this study were newly discovered in grain and were not identified in the previous proteome analysis conducted using the culms and leaves of B. distachyon. Therefore, the data obtained from all organs of B. distachyon infer a global cell wall proteome consisting of 460 proteins. At present, this is the most extensive cell wall proteome of a monocot species.

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Section: Discussionmentioning

confidence: 99%

Cell wall proteomic of Brachypodium distachyon grains: A focus on cell wall remodeling proteins

et al. 2015

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“…Proteins belonging to this family have not been studied in detail. However, they are suggested to be the homolog of the bactericidal/permeability increasing proteins in mammals (Gholizadeh and Kohnehrouz, 2013). DUF538 was proposed as a candidate protein that activates the plant response to abiotic and biotic stresses (Gholizadeh 2011).…”

Section: Downy Mildew Resistancementioning

confidence: 99%

Genetic diversity of Guatemalan climbing bean collections

Piñón¹,

Moghaddam

Lee

et al. 2020

Genet Resour Crop Evol

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Since common bean is the most important legume crop for human consumption around the world, bean breeders are challenged to increase production of beans while facing new problems like climate change. Guatemalan climbing beans have been suggested to represent race Guatemala, a newly identified race in the Middle American gene pool that may represent an untapped source of alleles for bean improvement. This study confirmed the existence of race Guatemala in the Middle American gene pool and its differentiation from other races. The low population structure found within these Guatemalan beans also makes this population ideal for discovery of candidate genes for important traits. We demonstrate that the Guatemalan population was useful to provide candidate genes for previously reported genetic factors like the V gene for flower color, and the Asp gene for seed coat luster. The important relationship between flowering time and altitudinal adaptation of beans was also emphasized. I would also like to acknowledge Dr. Samira Mafi Moghaddam, Rian Lee and Dr. Atena Oladzad from the Dry Bean Genomics Lab at NDSU for their instruction and help on each step of this project. Without their guidance, this project would have never been possible to conclude.

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“…Despite this, the nomination of DUF538 protein family as the potential structural and functional homologue of BPI protein in plants, not only provide a basis to study the potent biological functions of this protein family in the innate immune system of mammalians but also it open a new gate to investigate the homologues of DUF538 members in these organisms, too (Gholizadeh and Baghbankohnehrouz 2013).…”

Section: Distribution Of Duf538 In Naturementioning

confidence: 99%

“…DUF538 proteins are recently known to have the structural and functional signatures of bactericidal permeability increasing proteins in the immune system of mammalians (Gholizadeh and Baghbankohnehrouz 2013) and the water soluble chlorophyll binding proteins 1 in the photosynthetic apparatus of plants (Takahashi et al 2013). Later on, finding of structural similarity between BPI and esterase type hydrolase enzymes (Gholizadeh 2014) helped us to make this idea that DUF538 protein superfamily may be a member of hydrolases and degrades chlorophyll molecules in stress-challenged plants (considering DUF538 domain containing protein superfamily as stress-related proteins in plants).…”

Section: Structure-function Prediction Of Duf538mentioning

confidence: 99%

“…In a recent study, DUF538 proteins have been predicted as being potential homologues of BPI in mammalians immune system (Gholizadeh and Baghbankohnehrouz 2013). Thereby, they have been suggested to affect the bacterial growth rates through the binding to the LPS molecules on the outer leaflet of the bacterial membranes somewhat similar to BPI in mammalians innate immune system.…”

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confidence: 99%

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DUF538 protein superfamily is predicted to be chlorophyll hydrolyzing enzymes in plants

Gholizadeh

2015

Physiol Mol Biol Plants

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The possible hydrolytic activity towards chlorophyll molecules was predicted for DUF538 protein superfamily in plants. It was examined by using computational as well as experimental tools including in vitro chlorophyll degradation, antioxidant compounds production and in vivo real-time gene expression tests.Comparison of the computational data with the experimental results indicated that DUF538 proteins might be chlorophyll hydrolyzing enzyme (most probably carboxyesterase) which degrade chlorophyll molecules (66 % per 12 hrs) to produce new compounds (1.8 fold per 12 hrs) with antioxidant properties. The relevance of DUF538 gene expression level with the chlorophyll contents (2.8 fold increase per chlorophyll content of 50 %) of the drought-stressed leaves showed that chlorophyll degradation by DUF538 is most probably induced in response to stress stimuli. Despite membranous chlorophyll catabolic pathways, DUF538-dependent reactions is predicted to be occurred in the cytosol of the under stressed plants. We addressed as to whether chlorophyll breakdown to antioxidant compounds by DUF538 is a defense mechanism of plants against stress stimuli, in vivo? This question is going to be investigated in our next research project.

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DUF538 Protein Super Family is Predicted to be the Potential Homologue of Bactericidal/Permeability-Increasing Protein in Plant System

Cited by 17 publications

References 29 publications

Cell wall proteomic of Brachypodium distachyon grains: A focus on cell wall remodeling proteins

Cell wall proteomic of Brachypodium distachyon grains: A focus on cell wall remodeling proteins

Genetic diversity of Guatemalan climbing bean collections

DUF538 protein superfamily is predicted to be chlorophyll hydrolyzing enzymes in plants

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