Cell wall proteomic of <i>Brachypodium distachyon</i> grains: A focus on cell wall remodeling proteins

Glycoside hydrolases (GHs) are enzymes that are able to rearrange the plant cell wall polysaccharides, being developmental-and stress-regulated. Such proteins are used in enzymatic cocktails for biomass hydrolysis in the second-generation ethanol (E2G) production. In this chapter, we investigate GHs identified in plant cell wall proteomes by predicting their functions through alignment with homologous plant and microorganism sequences and identification of functional domains. Up to now, 49 cell wall GHs were identified in sugarcane and 114 in Brachypodium distachyon. We could point at candidate proteins that could be targeted to lower biomass recalcitrance. We more specifically addressed several GHs with predicted cellulase, hemicellulase, and pectinase activities, such as β-xylosidase, α and β-galactosidase, α-N-arabinofuranosidases, and glucan β-glucosidases. These enzymes are among the most used in enzymatic cocktails to deconstruct plant cell walls. As an example, the fungi arabinofuranosidases belonging to the GH51 family, which were also identified in sugarcane and B. distachyon, have already been associated to the degradation of hemicellulosic and pectic polysaccharides, through a peculiar mechanism, probably more efficient than other GH families. Future research will benefit from the information available here to design plant varieties with self-disassembly capacity, making the E2G more cost-effective through the use of more efficient enzymes.

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“…In B. distachyon, 114 CW GHs were identified in cell wall proteomes [31][32][33][34]. They are distributed into 21 families.…”

Section: Ghs Identified In Sugarcane and B Distachyon Cell Wall Protmentioning

confidence: 99%

“…Ten GH3s have been identified in sugarcane [28][29][30] and nine GH3s have been identified in B. distachyon [31,32,34]. Half of the sugarcane GH3 are predicted to have a β-glucosidase activity (GO:0008422) (e.g., SCEZLB1007A09, SCEQLR1093F09, and SCQSLR1089A04).…”

Section: Gh1 Gh3 and Gh51mentioning

confidence: 99%

Glycoside Hydrolases in Plant Cell Wall Proteomes: Predicting Functions That Could Be Relevant for Improving Biomass Transformation Processes

Calderan-Rodrigues¹,

Fonseca²,

Clemente³

et al. 2018

Advances in Biofuels and Bioenergy

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“…Many cell wall proteins (CWPs) have been identified by specific proteomic approaches (for recent reviews, see [13,14]). Regarding B. distachyon , several studies have been performed on young seedlings, leaves, stems and grain [15,16,17]. Among the identified CWPs, the glycoside hydrolases (GHs) are known to play a major role, with approximately twenty GH families potentially involved in cell wall remodelling, with glycosidase, glucanase and transglycosidase activities [18,19].…”

Section: Introductionmentioning

confidence: 99%

“…In a previous study, we investigated the cell wall proteome of the B. distachyon grain at a single developmental stage, i.e., the beginning of storage accumulation, and we highlighted numerous CWPs possibly involved in cell wall remodelling [16]. To increase the understanding of this process, we investigated the CWPs of the B. distachyon grain at three distinct developmental stages, starting at the end of cellularisation and ending at the beginning of the differentiation stage.…”

Section: Introductionmentioning

confidence: 99%

Understanding the Remodelling of Cell Walls during Brachypodium distachyon Grain Development through a Sub-Cellular Quantitative Proteomic Approach

et al. 2016

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Brachypodium distachyon is a suitable plant model for studying temperate cereal crops, such as wheat, barley or rice, and helpful in the study of the grain cell wall. Indeed, the most abundant hemicelluloses that are in the B. distachyon cell wall of grain are (1-3)(1-4)-β-glucans and arabinoxylans, in a ratio similar to those of cereals such as barley or oat. Conversely, these cell walls contain few pectins and xyloglucans. Cell walls play an important role in grain physiology. The modifications of cell wall polysaccharides that occur during grain development and filling are key in the determination of the size and weight of the cereal grains. The mechanisms required for cell wall assembly and remodelling are poorly understood, especially in cereals. To provide a better understanding of these processes, we purified the cell wall at three developmental stages of the B. distachyon grain. The proteins were then extracted, and a quantitative and comparative LC-MS/MS analysis was performed to investigate the protein profile changes during grain development. Over 466 cell wall proteins (CWPs) were identified and classified according to their predicted functions. This work highlights the different proteome profiles that we could relate to the main phases of grain development and to the reorganization of cell wall polysaccharides that occurs during these different developmental stages. These results provide a good springboard to pursue functional validation to better understand the role of CWPs in the assembly and remodelling of the grain cell wall of cereals.

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“…These organs were studied in order to compare the diferences between organs and to look for proteins possibly involved in cell wall extension and growth arrest. Grains were collected at diferent times after lowering , 13 or 1 days [31,61]. The aim of the study was to understand the modiications of cell wall polysaccharides during grain development and illing because they are key determinant of the size and mass of the grain.…”

Section: Plant Materialsmentioning

confidence: 99%

Cell Wall Proteomics as a Means to Identify Target Genes to Improve Second‐Generation Biofuel Production

Calderan-Rodrigues¹,

Fonseca²,

Labate³

et al. 2017

Frontiers in Bioenergy and Biofuels

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Second-generation biofuels "2G generally uses residues composed of lignocellulosic materials to produce renewable energy potentially up to 50% , without increasing the planted areas. However, the high cost of enzymes required for cell wall disassembly prior to the sacchariication makes the "2G production more expensive yet, compared to the irst-generation biofuels. Designing plants with less lignin, a barrier to "2G production, or facilitating cell wall disassembly by searching for the plant mechanisms can be the way to obtain "2G feasibility. Therewith, plant cell wall proteomics provides valuable information concerning the main cell wall proteins CWPs involved in its biosynthesis and rearrangements. Essentially, two plants of the grass family have been studied: sugarcane as a crop amenable to second-generation ethanol E2G production and Brachypodium distachyon as a model plant amenable to genetic transformation. Cell wall proteomics has allowed the identiication of numerous CWPs as well as their ine proiling in diferent organs and at various developmental stages. Proteins acting on carbohydrates, mostly glycosyl hydrolases, and oxidoreductases, including class III peroxidases and laccases, can be highlighted. "oth kinds of CWPs are assumed to contribute to the remodelling of cell wall polysaccharides by enzymatic or nonenzymatic mechanisms. CWPs present in growing organs could also be atractive candidates since they greatly contribute to cell wall plasticity.Keywords: Brachypodium distachyon, cell wall protein, grass, second generation ethanol, sugarcane © 2017 The Author(s). Licensee InTech. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.© 2017 The Author(s). Licensee InTech. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. . IntroductionSecond-generation biofuels "2G are a promising renewable alternative to supply energy demand of fossil fuels worldwide, whose advantage is mostly due to the lower emission of greenhouse gases and the possibility to increase the production without widening the planted area. However, we are still far from producing "2G at an economically competitive way and reasonable amount to replace fossil fuels. "2G uses lignocellulosic material as substrates. Since sugarcane has been considered one of the best crops to produce bioethanol, its bagasse and straw have been studied as one of the main complementary sources of C 6 and C 5 sugars for "2G. One of the main constrains to its economic feasibility relies on the rate of success of the enzymatic sacchariication enabling the conversion of the plant cell wall sugars into bioethanol [1]. Sacchariication of the cell wall ...

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Cell wall proteomic of Brachypodium distachyon grains: A focus on cell wall remodeling proteins

Cited by 27 publications

References 68 publications

Glycoside Hydrolases in Plant Cell Wall Proteomes: Predicting Functions That Could Be Relevant for Improving Biomass Transformation Processes

Glycoside Hydrolases in Plant Cell Wall Proteomes: Predicting Functions That Could Be Relevant for Improving Biomass Transformation Processes

Understanding the Remodelling of Cell Walls during Brachypodium distachyon Grain Development through a Sub-Cellular Quantitative Proteomic Approach

Cell Wall Proteomics as a Means to Identify Target Genes to Improve Second‐Generation Biofuel Production

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