Dual release of dexamethasone and TGF-β3 from polymeric microspheres for stem cell matrix accumulation in a rat disc degeneration model

Liang, Chengzhen; Li, Hao; Tao, Yiqing; Peng, Li‐Hua; Gao, Jian; Wu, Jingjun; Li, Fangcai; Hua, Jianming; Chen, Qixin

doi:10.1016/j.actbio.2013.08.019

Cited by 65 publications

(51 citation statements)

References 55 publications

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“…This has been used in both animal and human studies. 80 In addition, fibrin sealant has also been used for preventing leakage. 39,60Y65 In a pilot human study, a single direct injection of MSCs into the NP of the affected segment was performed.…”

Section: Msc Transplantation Techniques and The Use Of Cell Carriermentioning

confidence: 99%

Stem Cell Therapy for Intervertebral Disk Regeneration

Gou

Oxentenko

Eldrige

et al. 2014

American Journal of Physical Medicine &Amp; Rehabilitation

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Intervertebral disk degeneration has been considered an irreversible process characterized by a decrease in cell viability, attenuation of proteoglycan and type II collagen synthesis, and dehydration of nucleus pulposus. Stem cell therapy specifically addresses the degenerative process and offers a potentially effective treatment modality. Current preclinical studies show that mesenchymal stem cells have the capacity to repair degenerative disks by differentiation toward chondrocyte-like cells, which produce proteoglycans and type II collagen. There has been evidence that mesenchymal stem cell transplantation into the intervertebral disk increases the intradiskal magnetic resonance imaging T2 signal intensity, increases the disk height, and decreases the degenerative grade in animal models. Appropriate selection of cell carriers/matrix is important because it may prevent cell leakage into the spinal canal and provide an environment that facilitates cell proliferation and differentiation. Although human cell therapy trials for degenerative disk disease are on the horizon, potential issues might arise. The authors hereby review the current state of regenerative cell therapy in degenerative disk disease, with emphasis in cell source, techniques for cellular expansion, induction, transplantation, potential benefit, and risks of the use of this novel medical armamentarium in the treatment of degenerative disk disease.

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Section: Msc Transplantation Techniques and The Use Of Cell Carriermentioning

confidence: 99%

Stem Cell Therapy for Intervertebral Disk Regeneration

Gou

Oxentenko

Eldrige

et al. 2014

American Journal of Physical Medicine &Amp; Rehabilitation

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“…The present study focused on the synergy between TGF-b3 and insulin-like growth factor 1 (IGF-1). TGF-b3, which is involved in cell differentiation, adhesion and ECM formation (Dudas et al, 2004), plays an essential role in the development and function of cartilaginous organs (Pelton et al, 1991), including intervertebral discs (Risbud et al, 2006), and has been used in chondrogenic differentiation of multiple stem cell types (Bian et al, 2011;Liang et al, 2013). In addition, recent studies have shown the function of IGFRs on NP cells in intervertebral disc development and ECM synthesis (Le Maitre et al, 2005), and demonstrated that IGF-1 is important in development process (Okuda et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

TGF-β3 and IGF-1 synergy ameliorates nucleus pulposus mesenchymal stem cell differentiation towards the nucleus pulposus cell type through MAPK/ERK signaling

et al. 2015

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This study aimed to investigate the synergy between transforming growth factor beta 3 (TGF-β3) and insulin-like growth factor 1 (IGF-1) on nucleus pulposus-derived mesenchymal stem cells (NP-MSCs) and the underlying mechanism using a serum-free culture system. NP-MSC proliferation and viability were measured using a CCK-8 assay and annexin V-FITC/propidium iodide, respectively. NP-MSCs in micromasses were investigated for differentiation towards nucleus pulposus cells (NPCs). SOX-9, collagen-I, collagen-II, aggrecan and decorin expressions were detected by RT-PCR and immunoblotting. Matrix deposition was assessed by sulfated glycosaminoglycan (sGAG) analysis. Novel chondrogenic and nucleus pulposus (NP) genes were detected to distinguish differentiated cell types. MAPK/ERK and TGF/Smad signaling pathways were also examined. As a result, the synergy between TGF-β3 and IGF-1 enhanced NP-MSC viability, extracellular matrix (ECM) biosynthesis and differentiation towards NPCs, partly through the activation of the MAPK/ERK signaling pathway. Therefore, the synergy between TGF-β3 and IGF-1 ameliorates NP-MSC viability, differentiation and promotes intervertebral disc regeneration.

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“…Rats were chosen as the experimental model since they are commonly used in IVD research [24][25][26]. Considering that the perichondrium region adjacent to the EP may be the common origin of stem cells in the NP, CEP, and AF (including AFo), we chose this region as the target ISN area [24].…”

Section: Introductionmentioning

confidence: 99%

The presence of stem cells in potential stem cell niches of the intervertebral disc region: an in vitro study on rats

et al. 2015

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To the best of our knowledge, this is the first in vitro study aimed towards determining the existence and characteristics of ISN-SCs, which belong to the MSC family and with greater osteogenic and chondrogenic abilities than BMSCs according to our data. This finding may be of great significance for additional studies that investigate the migration of ISN-SCs into the IVD, and may provide a new perspective on different biological approaches for IVD self-regeneration.

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Dual release of dexamethasone and TGF-β3 from polymeric microspheres for stem cell matrix accumulation in a rat disc degeneration model

Cited by 65 publications

References 55 publications

Stem Cell Therapy for Intervertebral Disk Regeneration

Stem Cell Therapy for Intervertebral Disk Regeneration

TGF-β3 and IGF-1 synergy ameliorates nucleus pulposus mesenchymal stem cell differentiation towards the nucleus pulposus cell type through MAPK/ERK signaling

The presence of stem cells in potential stem cell niches of the intervertebral disc region: an in vitro study on rats

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