Dual optical biosensors for imaging microRNA-1 during myogenesis

Kang, Won Jun; Cho, Ye Lim; Chae, Ju Ri; Lee, Jong Doo; Ali, Bahy A.; Al-Khedhairy, Abdulaziz A.; Lee, Young Moo; Kim, Soonhag

doi:10.1016/j.biomaterials.2012.05.056

Cited by 27 publications

(26 citation statements)

References 37 publications

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“…Up to now, only a few of concerns are in regards to the miRNA imaging. For example, Kim et al have reported a serious of imaging probes to monitor cellular miRNAs based on the FRET (Kang et al, 2012;Kim et al, 2012). Ju et al have developed a polyethylenimine-grafted graphene nanoribbon for cellular delivery of locked nucleic acid modified molecular beacon for recognization of miRNA (Dong et al, 2011).…”

Section: Introductionmentioning

confidence: 98%

A Graphene-enhanced imaging of microRNA with enzyme-free signal amplification of catalyzed hairpin assembly in living cells

Liu

Tian

et al. 2016

Biosensors and Bioelectronics

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Section: Introductionmentioning

confidence: 98%

A Graphene-enhanced imaging of microRNA with enzyme-free signal amplification of catalyzed hairpin assembly in living cells

Liu

Tian

et al. 2016

Biosensors and Bioelectronics

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“…Most sensors were introduced into cells using a lipid-based delivery system, which may bias the delivery of the sensors to the endocytic pathway of the cell destined for degradation. In fact, the pattern of miRNA expression in punctate structures shown by these studies differs substantially from in situ hybridization experiments (3)(4)(5)(6)(7)(8)(9)(10). Among the many types of sensors used in tracking miRNAs, molecular beacons hold the greatest promise because of their high specificity toward small sequences of RNA and low signal to noise ratio (11,12).…”

Section: Micrornas (Mirnas)mentioning

confidence: 76%

Dual Functional Roles of Molecular Beacon as a MicroRNA Detector and Inhibitor

Chan

Miller

et al. 2017

Journal of Biological Chemistry

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Edited by Xiao-Fan WangMicroRNAs are essential in many cellular processes. The ability to detect microRNAs is important for understanding its function and biogenesis. This study is aimed at using a molecular beacon to detect miR-430 in developing zebrafish embryos as a proof of principle. miR-430 is crucial for the clearance of maternal mRNA during maternal zygotic transition in embryonic development. Despite its known function, the temporal and spatial expression of miR-430 remains unclear. We used various imaging techniques, including laser scanning confocal microscopy, spinning disk, and lightsheet microscopy, to study the localization of miR-430 and any developmental defects possibly caused by the molecular beacon. Our results show that miR-430 is expressed early in development and is localized in distinct cytoplasmic granules where its target mRNA can be detected. We also show that the designed molecular beacon can inhibit the function of miR-430 and cause developmental defect in the brain, notochord, heart, and kidney, depending on the delivery site within the embryo, suggesting that miR-430 plays a diverse role in embryonic morphogenesis. When compared with morpholino, molecular beacon is 2 orders of magnitude more potent in inhibiting miR-430. Thus, our results reveal that in addition to being used as a valuable tool for the detection of microRNAs in vivo, molecular beacons can also be employed to inhibit microRNAs in a specific manner. MicroRNAs (miRNAs)2 are a class of short, non-coding RNAs involved in the regulation of gene expression at the posttranscriptional level. MiRNAs are implicated in many diseases and are essential in many cellular processes including development (1). Understanding the spatial expression of these small RNAs inside the cell can provide important information about their molecular function. The use of in situ hybridization to study intracellular localization of miRNAs in fixed cells has technical limitations because of the small size and low quantities of the RNA (2). In addition, an unusual high background, possibly contributed by nonspecific binding to DNA, can make interpretation of results difficult (3). Live imaging of miRNAs has been attempted in many studies, but success remains questionable, mainly because of challenges in the delivery of miRNA sensors to the appropriate compartment inside the cell. Most sensors were introduced into cells using a lipid-based delivery system, which may bias the delivery of the sensors to the endocytic pathway of the cell destined for degradation. In fact, the pattern of miRNA expression in punctate structures shown by these studies differs substantially from in situ hybridization experiments (3-10). Among the many types of sensors used in tracking miRNAs, molecular beacons hold the greatest promise because of their high specificity toward small sequences of RNA and low signal to noise ratio (11,12). Furthermore, molecular beacons are able to provide real time signals without relying on other indirect and complicated detection systems suc...

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“…These methods are important in the early study and precise diagnosis of miRNA involving-cellular developments and diseases. We successfully developed and demonstrated the ColoR MB, which provides target miRNA expression-dependent color change and overcomes the limitations of prior miRNA imaging systems [4,7,8,13,14,17] . The ColoR MB clearly increased fluorescence signals of the reporter probe in the presence of target miRNAs and consistently visualized fluorescence brightness of the reference probe irrespective of miRNA expression [7,8] .…”

mentioning

confidence: 99%

“…The bioluminescent miRNA reporter gene was designed to detect target miRNAs expressions by cloning perfectly complementary sequences of mature miRNA in the 3'UTR of luciferase [5] . There have been successive reports on miRNA reporter gene imaging systems for monitoring miR-1, miR-9, miR-23a, miR-221, and miR124a biogenesis, including myogenesis, neurogenesis, and carcinogenesis [13][14][15][16][17] . In the presence of target miRNAs, the imaging system is a signal-off system that operates through destabilization of the luciferase reporter gene by miRNA of interest [4,5,13,16,18] .…”

mentioning

confidence: 99%

“…We successfully visualized expressions of miR-1, miR-26a, miR124a, miR-126, miR-206, and miR-221 by miR MBs in vitro and in vivo. These miRNAs are highly expressed during myogenesis, neurogenesis, and carcinogenesis [4,6,14,19] . However, it is still difficult to obtain accurate information from the mono-fluorophore-based miR MB when fluorescence intensity in cells is relatively weak.…”

mentioning

confidence: 99%

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